A Conserved Telomerase Motif within the Catalytic Domain of Telomerase Reverse Transcriptase Is Specifically Required for Repeat Addition Processivity

Molecular and Cellular Biology

Volumn 23, Issue 23, 2003, Pages 8440-8449

  Lue, Neal F ^a   Lin, You Chin ^a   Mian, I Saira ^b  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; RNA DIRECTED DNA POLYMERASE; TELOMERASE;

ARTICLE; CONTROLLED STUDY; DNA BINDING; ENZYME ACTIVITY; GENE INSERTION; GENE MUTATION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; TELOMERE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CONSERVED SEQUENCE; DNA-BINDING PROTEINS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TELOMERASE; TELOMERE;

INSERTION SEQUENCES; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0242552586     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.23.23.8440-8449.2003     Document Type: Article

References (53)

1. Beattie, T. L., W. Zhou, M. O. Robinson, and L. Harrington. 2000. Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding. Mol. Biol. Cell 11:3329-3340.
2. Beattie, T. L., W. Zhou, M. O. Robinson, and L. Harrington. 1998. Reconstitution of human telomerase activity in vitro. Curr. Biol. 8:177-180.
3. Bednenko, J., M. Melek, E. C. Greene, and D. E. Shippen. 1997. Developmentally regulated initiation of DNA synthesis by telomerase: evidence for factor-assisted de novo telomere formation. EMBO J. 16:2507-2518.
4. Blackburn, E. H. 1992. Telomerases. Annu. Rev. Biochem. 61:113-129.
5. Bosoy, D., and N. Lue. 2001. Functional analysis of conserved residues in the putative "finger" domain of telomerase reverse transcriptase. J. Biol. Chem. 276:46305-46312.
6. Bryan, T. M., K. J. Goodrich, and T. R. Cech. 2000. A mutant of Tetrahymena telomerase reverse transcriptase with increased processivity. J. Biol. Chem. 275:24199-24207.
7. Bryan, T. M., J. M. Sperger, K. B. Chapman, and T. R. Cech. 1998. Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytrica trifallax. Proc. Natl. Acad. Sci. 95:8479-8484.
8. Chen, J., and C. Greider. 2003. Determinants in mammalian telomerase RNA that mediate enzyme processivity and cross-species incompatibility. EMBO J. 22:304-314.
9. Chen, J. L., M. A. Blasco, and C. W. Greider. 2000. Secondary structure of vertebrate telomerase RNA. Cell 100:503-514.
10. Cohn, M., and E. H. Blackburn. 1995. Telomerase in yeast. Science 269:396-400.
11. Collins, K. 1999. Ciliate telomerase biochemistry. Annu. Rev. Biochem. 68:187-218.
12. Collins, K., and L. Gandhi. 1998. The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex. Proc. Natl. Acad. Sci. 95:8485-8490.
13. Collins, K., R. Kobayashi, and C. W. Greider. 1995. Purification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme. Cell 81:677-686.
14. Fitzgerald, M. S., K. Riha, F. Gao, S. Ren, T. D. McKnight, and D. E. Shippen. 1999. Disruption of the catalytic telomerase subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA. Proc. Natl. Acad. Sci. 96:14813-14818.
15. Forstemann, K., M. Hoss, and J. Lingner. 2000. Telomerase-dependent repeat divergence at the 3′ ends of yeast telomeres. Nucleic Acids Res. 28:2690-2694.
16. Fulton, T. B., and E. H. Blackburn. 1998. Identification of Kluyveromyces lactis telomerase: discontinuous synthesis along the 30-nucleotide-long templating domain. Mol. Cell. Biol. 18:4961-4970.
17. Gandhi, L., and K. Collins. 1998. Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substrates. Genes Dev. 12:721-733.
18. Greenberg, R. A., R. C. Allsopp, L. Chin, G. B. Morin, and R. A. DePinho. 1998. Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation. Oncogene 16:1723-1730.
19. Greene, E. C., and D. E. Shippen. 1998. Developmentally programmed assembly of higher order telomerase complexes with distinct biochemical and structural properties. Genes Dev. 12:2921-2931.
20. Greider, C. 1991. Telomerase is processive. Mol. Cell. Biol. 11:4572-4580.
21. Greider, C. W., and E. H. Blackburn. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413.
22. Greider, C. W., and E. H. Blackburn. 1989. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337:331-337.
23. Harrington, L. 2003. Biochemical aspects of telomerase function. Cancer Lett. 194:139-154.
24. Harrington, L., T. McPhail, V. Mar, W. Zhou, R. Oulton, A. E. program, M. B. Bass, I. Arruda, and M. O. Robinson. 1997. A mammalian telomerase-associated protein. Science 275:973-977.
25. Holt, S. E., D. L. Aisner, J. Baur, V. M. Tesmer, M. Dy, M. Ouellette, J. B. Trager, G. B. Morin, D. O. Toft, J. W. Shay, W. E. Wright, and M. A. White. 1999. Functional requirement of p23 and Hsp90 in telomerase complexes. Genes Dev. 13:817-826.
26. Hossain, S., S. Singh, and N. Lue. 2002. Functional analysis of the C-terminal extension of telomerase reverse transcriptase: a putative "thumb" domain. J. Biol. Chem. 277:36174-36180.
27. Huang, H., R. Chopra, G. L. Verdine, and S. C. Harrison. 1998. Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. Science 282:1669-1675.
28. Karamysheva, Z., L. Wang, T. Shrode, J. Bednenko, L. Hurley, and D. Shippen. 2003. Developmentally programmed gene elimination in euplotes crassus facilitates a switch in the telomerase catalytic subunit. Cell 113:565-576.
29. Kelleher, C., M. Teixeira, K. Forstemann, and J. Lingner. 2002. Telomerase: biochemical considerations for enzyme and substrate. Trends Biochem. Sci. 27:572-579.
30. Kilian, A., D. D. Bowtell, H. E. Abud, G. R. Hime, D. J. Venter, P. K. Keese, E. R. Duncan, R. R. Reddel, and R. A. Jefferson. 1997. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types. Hum. Mol. Genet. 6:2011-2019.
31. Lendvay, T. S., D. K. Morris, J. Sah, B. Balasubramanian, and V. Lundblad. 1996. Senescence mutants of saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 144:1399-1412.
32. Lingner, J., and T. R. Cech. 1996. Purification of telomerase from Euplotes aediculatis: requirement of a primer 3′ overhang. Proc. Natl. Acad. Sci. 93:10712-10717.
33. Lingner, J., T. R. Hughes, A. Shevchenko, M. Mann, V. Lundblad, and T. R. Cech. 1997. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276:561-567.
34. Lue, N., 2002. Yeast telomerases: structure, mechanisms and regulation, p. 239-258. In G. Krupp and R. Parwaresh (ed.), Telomerases, telomeres and cancer. Landes Biosciences, Georgetown, Tex.
35. Lue, N. F., and R. D. Kornberg. 1987. Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. 84:8839-8843.
36. Lue, N. F., and Y. Peng. 1998. Negative regulation of yeast telomerase activity through an interaction with an upstream region of the DNA primer. Nucleic Acids Res. 26:1487-1494.
37. Lue, N. F., and J. Xia. 1998. Species-specific and sequence-specific recognition of the dG-rich strand of telomeres by yeast telomerase. Nucleic Acids Res. 26:1495-1502.
38. Marcand, S., V. Brevet, and E. Gilson. 1999. Progressive cis-inhibition of telomerase upon telomere elongation. EMBO J. 18:3509-3519.
39. Melton, D. A., P. A. Krieg, M. R. Rebagliati, T. Maniatis, K. Zinn, and M. R. Green. 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12:7035-7056.
40. Meyerson, M., C. M. Counter, E. N. Eaton, L. W. Ellisen, P. Steiner, S. D. Caddle, L. Ziaugra, R. L. Beijersbergen, M. J. Davidoof, and Q. e. a. Liu. 1997. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 90:785-795.
41. Nakamura, T. M., G. B. Morin, K. B. Chapman, S. L. Weinrich, W. H. Andrews, J. Lingner, C. B. Harley, and T. R. Cech. 1997. Telomerase catalytic subunit homologs from fission yeast and human. Science 277:955-959.
42. Nakayama, J.-I., M. Saito, H. Nakamura, A. Matsuura, and F. Ishikawa. 1997. TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family. Cell 88:875-884.
43. Nugent, C. I., and V. Lundblad. 1998. The telomerase reverse transcriptase: components and regulation. Genes Dev. 12:1073-1085.
44. Oguchi, K., H. Liu, K. Tamura, and H. Takahashi. 1999. Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana. FEBS Lett. 457:465-469.
45. Peng, Y., I. S. Mian, and N. F. Lue. 2001. Analysis of telomerase processivity: mechanistic similarity to HIV-1 reverse transcriptase and role in telomere maintenance. Mol. Cell 7:1201-1211.
46. Pennock, E., K. Buckley, and V. Lundblad. 2001. Cdc13 delivers separate complexes to the telomere for end protection and replication. Cell 104:387-396.
47. Prescott, J., and E. H. Blackburn. 1997. Telomerase RNA mutations in Saccharomyces cerevisiae alter telomerase action and reveal non-processivity in vivo and in vitro. Genes Dev. 11:528-540.
48. Singer, M. S., and D. E. Gottschling. 1994. TLC1: template RNA component of Saccharomyces cerevisiae telomerase. Science 266:404-409.
49. Singh, S., O. Steinberg-Neifach, I. Mian, and N. Lue. 2002. Analysis of telomerase in Candida albicans: potential role in telomere end protection. Eukaryot. Cell 1:967-977.
50. Tzfati, Y., T. B. Fulton, J. Roy, and E. H. Blackburn. 2000. Template boundary in a yeast telomerase specified by RNA structure. Science 288:863-867.
51. Weinrich, S. L., R. Pruzan, L. Ma, M. Ouellette, V. M. Tesmer, S. E. Holt, A. G. Bodnar, S. Lichtsteiner, N. W. Kim, J. B. Trager, R. D. Taylor, R. Carlos, W. H. Andrews, W. E. Wright, J. W. Shay, C. B. Harley, and G. B. Morin. 1997. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nat. Genet. 17:198-502.
52. Xia, J., Y. Peng, I. S. Mian, and N. F. Lue. 2000. Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase Mol. Cell. Biol. 20:5196-5207.
53. Zhu, J., H. Wang, J. M. Bishop, and E. H. Blackburn. 1999. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening. Proc. Natl. Acad. Sci. 96:3723-3728.