Pyruvate ameliorates post ischemic injury of rat astrocytes and protects them against PARP mediated cell death

Brain Research

Volumn 992, Issue 1, 2003, Pages 104-113

  Sharma, Pushpa ^a   Karian, John ^a   Sharma, Swapnil ^a   Liu, Suzhen ^a   Mongan, Paul D ^a  

^a UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES   (United States)

Author keywords

Apoptosis; Astrocyte; Gliosis; Ischemia; PARP poly(ADP ribose)polymerase ; Pyruvate; Pyruvate dehydrogenase; Reperfusion injury

Indexed keywords

ADENOSINE TRIPHOSPHATE; BUFFER; CASPASE 3; CYTOCHROME C; GLIAL FIBRILLARY ACIDIC PROTEIN; GLUCOSE; LACTATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE INHIBITOR; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVIC ACID;

ANIMAL CELL; APOPTOSIS; ARTICLE; ASTROCYTE; BLOOD OXYGENATION; BRAIN INJURY; BRAIN ISCHEMIA; BRAIN PERFUSION; CELL DAMAGE; CELL DEATH; CELL LEVEL; CELL METABOLISM; CITRIC ACID CYCLE; CONTROLLED STUDY; DISEASE COURSE; ENZYME INHIBITION; ENZYME RELEASE; GLIOSIS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; INCUBATION TIME; NEUROPROTECTION; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; RAT;

EID: 0242290007     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2003.08.043     Document Type: Article

References (51)

1. Abramson D., Scalea T.M., Hitchcock R., Trooskin S.Z., Henry S.M., Greenspan J. Lactate clearance and survival following injury. J. Trauma. 35:1993;584-588. (discussion 588-589).
2. Bogaert Y.E., Rosenthal R.E., Fiskum G. Postischemic inhibition of cerebral cortex pyruvate dehydrogenase. Free Radic. Biol. Med. 16:1994;811-820.
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
4. Brooks G.A. Lactate shuttles in nature. Biochem. Soc. Trans. 30:2002;258-264.
5. Cardell M., Koide T., Wieloch T. Pyruvate dehydrogenase activity in the rat cerebral cortex following cerebral ischemia. J. Cereb. Blood Flow. Metab. 9:1989;350-357.
6. Desagher S., Glowinski J., Premont J. Pyruvate protects neurons against hydrogen peroxide-induced toxicity. J. Neurosci. 17:1997;9060-9067.
7. Erecinska M., Silver I.A. ATP and brain function. J. Cereb. Blood Flow Metab. 9:1989;2-19.
8. Garland J.M., Halestrap A. Energy metabolism during apoptosis. Bcl-2 promotes survival in hematopoietic cells induced to apoptose by growth factor withdrawal by stabilizing a form of metabolic arrest. J. Biol. Chem. 272:1997;4680-4688.
9. Gisselsson L., Smith M.L., Siesjo B.K. Hyperglycemia and focal brain ischemia. J. Cereb. Blood Flow Metab. 19:1999;288-297.
10. Hansford R.G. Studies on inactivation of pyruvate dehydrogenase by palmitoylcarnitine oxidation in isolated rat heart mitochondria. J. Biol. Chem. 252:1977;1552-1560.
11. Hayashi T., Sakurai M., Abe K., Itoyama Y. DNA fragmentation precedes aberrant expression of cell cycle-related protein in rat brain after MCA occlusion. Neurol. Res. 21:1999;695-698.
12. Hinman L.M., Blass J.P. An NADH-linked spectrophotometric assay for pyruvate dehydrogenase complex in crude tissue homogenates. J. Biol. Chem. 256:1981;6583-6586.
13. Hirai K., Sugawara T., Chan P.H., Basus V.J., James T.L., Litt L. Cytochrome c associated apoptosis during ATP recovery after hypoxia in neonatal rat cerebrocortical slices. J. Neurochem. 83:2002;309-319.
14. Jones K.H., Senft J.A. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. J. Histochem. Cytochem. 33:1985;77-79.
15. Juurlink B.H., Hertz L. Ischemia-induced death of astrocytes and neurons in primary culture: pitfalls in quantifying neuronal cell death. Brain Res. Dev. Brain Res. 71:1993;239-246.
16. Juurlink B.H., Paterson P.G. Review of oxidative stress in brain and spinal cord injury: suggestions for pharmacological and nutritional management strategies. J. Spinal. Cord. Med. 21:1998;309-334.
17. Kauppinen R.A., Enkvist K., Holopainen I., Akerman K.E. Glucose deprivation depolarizes plasma membrane of cultured astrocytes and collapses transmembrane potassium and glutamate gradients. Neuroscience. 26:1988;283-289.
18. Kerbey A.L., Randle P.J., Cooper R.H., Whitehouse S., Pask H.T., Denton R.M. Regulation of pyruvate dehydrogenase in rat heart. Mechanism of regulation of proportions of dephosphorylated and phosphorylated enzyme by oxidation of fatty acids and ketone bodies and of effects of diabetes: role of coenzyme A, acetyl-coenzyme A and reduced and oxidized nicotinamide-adenine dinucleotide. Biochem. J. 154:1976;327-348.
19. Korbo L. Glial cell loss in the hippocampus of alcoholics. Alcohol. Clin. Exp. Res. 23:1999;164-168.
20. Landis D.M. The early reactions of non-neuronal cells to brain injury. Annu. Rev. Neurosci. 17:1994;133-151.
21. Li P.A., Gisselsson L., Keuker J., Vogel J., Smith M.L., Kuschinsky W., Siesjo B.K. Hyperglycemia-exaggerated ischemic brain damage following 30 min of middle cerebral artery occlusion is not due to capillary obstruction. Brain Res. 804:1998;36-44.
22. Lust W.D., Taylor C., Pundik S., Selman W.R., Ratcheson R.A. Ischemic cell death: dynamics of delayed secondary energy failure during reperfusion following focal ischemia. Metab. Brain Dis. 17:2002;113-121.
23. Maeda K., Hata R., Gillardon F., Hossmann K.A. Aggravation of brain injury after transient focal ischemia in p53-deficient mice. Brain Res. Mol. Brain Res. 88:2001;54-61.
24. Malhotra R., Lin Z., Vincenz C., Brosius F.C. III Hypoxia induces apoptosis via two independent pathways in Jurkat cells: differential regulation by glucose. Am. J. Physiol. Cell. Physiol. 281:2001;C1596-C1603.
25. Mongan P.D., Capacchione J., Fontana J.L., West S., Bunger R. Pyruvate improves cerebral metabolism during hemorrhagic shock. Am. J. Physiol. Heart. Circ. Physiol. 281:2001;H854-H864.
26. Mongan P.D., Capacchione J., West S., Karaian J., Dubois D., Keneally R., Sharma P. Pyruvate improves redox status and decreases indicators of hepatic apoptosis during hemorrhagic shock in swine. Am. J. Physiol. Heart Circ. Physiol. 283:2002;H1634-H1644.
27. Mongan P.D., Karaian J., Van Der Schuur B.M., Via D.K., Sharma P. Pyruvate prevents poly-ADP ribose polymerase (PARP) activation, oxidative damage, and pyruvate dehydrogenase deactivation during hemorrhagic shock in swine. J. Surg. Res. 112:2003;180-188.
28. Niknahad H., Khan S., O'Brien P.J. Hepatocyte injury resulting from the inhibition of mitochondrial respiration at low oxygen concentrations involves reductive stress and oxygen activation. Chem. Biol. Interact. 98:1995;27-44.
29. Norenberg M.D. Astrocyte responses to CNS injury. J. Neuropathol. Exp. Neurol. 53:1994;213-220.
30. O'Donnell-Tormey J., Nathan C.F., Lanks K., DeBoer C.J., de la Harpe J. Secretion of pyruvate. An antioxidant defense of mammalian cells. J. Exp. Med. 165:1987;500-514.
31. Perez-Pinzon M.A., Xu G.P., Born J., Lorenzo J., Busto R., Rosenthal M., Sick T.J. Cytochrome C is released from mitochondria into the cytosol after cerebral anoxia or ischemia. J. Cereb. Blood Flow. Metab. 19:1999;39-43.
32. Petito C.K., Juurlink B.H., Hertz L. In vitro models differentiating between direct and indirect effects of ischemia on astrocytes. Exp. Neurol. 113:1991;364-372.
33. Randle P.J. Metabolic fuel selection: general integration at the whole-body level. Proc. Nutr. Soc. 54:1995;317-327.
34. Reichert S.A., Kim-Han J.S., Dugan L.L. The mitochondrial permeability transition pore and nitric oxide synthase mediate early mitochondrial depolarization in astrocytes during oxygen-glucose deprivation. J. Neurosci. 21:2001;6608-6616.
35. Schafer F.Q., Buettner G.R. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radic. Biol. Med. 30:2001;1191-1212.
36. Shao Y., McCarthy K.D. Plasticity of astrocytes. Glia. 11:1994;147-155.
37. Sharma P. Effect of ascorbic acid on hyperoxic rat astrocytes. Neuroscience. 72:1996;391-397.
38. Sokoloff L. Neurophysiology and neurochemistry of coma. Exp. Biol. Med. 4:1971;15-33.
39. Sonnewald U., Muller T.B., Westergaard N., Unsgard G., Petersen S.B., Schousboe A. NMR spectroscopic study of cell cultures of astrocytes and neurons exposed to hypoxia: compartmentation of astrocyte metabolism. Neurochem. Int. 24:1994;473-483.
40. Sulkowski G., Bubko I., Struzynska L., Januszewski S., Walski M., Rafalowska U. Astrocytic response in the rodent model of global cerebral ischemia and during reperfusion. Exp. Toxicol. Pathol. 54:2002;31-38.
41. Swanson R.A., Farrell K., Stein B.A. Astrocyte energetics, function, and death under conditions of incomplete ischemia: a mechanism of glial death in the penumbra. Glia. 21:1997;142-153.
42. Tombaugh G.C., Sapolsky R.M. Evolving concepts about the role of acidosis in ischemic neuropathology. J. Neurochem. 61:1993;793-803.
43. Vistica D.T., Skehan P., Scudiero D., Monks A., Pittman A., Boyd M.R. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res. 51:1991;2515-2520.
44. Walz W. Role of astrocytes in the clearance of excess extracellular potassium. Neurochem. Int. 36:2000;291-300.
45. Wiessner C., Sauer D., Alaimo D., Allegrini P.R. Protective effect of a caspase inhibitor in models for cerebral ischemia in vitro and in vivo. Cell Mol. Biol. (Noisy-le-grand). 46:2000;53-62.
46. Wilding M., Fiorentino A., De Simone M.L., Infante V., De Matteo L., Marino M., Dale B. Energy substrates, mitochondrial membrane potential and human preimplantation embryo division. Reprod. Biomed. Online. 5:2002;39-42.
47. Wu V.W., Schwartz J.P. Cell culture models for reactive gliosis: new perspectives. J. Neurosci. Res. 51:1998;675-681.
48. Yang G.Y., Pang L., Ge H.L., Tan M., Ye W., Liu X.H., Huang F.P., Wu D.C., Che X.M., Song Y., Wen R., Sun Y. Attenuation of ischemia-induced mouse brain injury by SAG, a redox-inducible antioxidant protein. J. Cereb. Blood Flow. Metab. 21:2001;722-733.
49. Ying W., Sevigny M.B., Chen Y., Swanson R.A. Poly(ADP-ribose) glycohydrolase mediates oxidative and excitotoxic neuronal death. Proc. Natl. Acad. Sci. U. S. A. 98:2001;12227-12232.
50. Yu A.C., Wong H.K., Yung H.W., Lau L.T. Ischemia-induced apoptosis in primary cultures of astrocytes. Glia. 35:2001;121-130.
51. Yu A.C., Lau A.M., Fu A.W., Lau L.T., Lam P.Y., Chen X.Q., Xu Z.Y. Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia. Neurochem. Res. 27:2002;1663-1668.