Engineering of Dendrimer Surfaces to Enhance Transepithelial Transport and Reduce Cytotoxicity

Pharmaceutical Research

Volumn 20, Issue 10, 2003, Pages 1543-1550

  Jevprasesphant, Rachaneekorn ^a   Penny, Jeffrey ^a   Attwood, David ^a   McKeown, Neil B ^a   D'Emanuele, Antony ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Caco 2 cells; Cytotoxicity; Dendrimer surface modification; Engineering of dendrimer surfaces; PAMAM dendrimer

Indexed keywords

AMINE; ANION; CATION; CHLORIDE; COLCHICINE; DENDRIMER; EDETIC ACID; LAURYL CHLORIDE; LIPID; MANNITOL; POLYAMIDODIAMINE; UNCLASSIFIED DRUG;

ARTICLE; CELL LINE; COLON ADENOCARCINOMA; CONTROLLED STUDY; CYTOTOXICITY; ELECTRIC RESISTANCE; HUMAN; HUMAN CELL; MEASUREMENT; PRIORITY JOURNAL; PROTEIN TRANSPORT;

BIOLOGICAL TRANSPORT; CACO-2 CELLS; CELL SURVIVAL; DRUG DESIGN; ELECTRIC CONDUCTIVITY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; INTESTINAL MUCOSA; LAURATES; MODELS, BIOLOGICAL; PERMEABILITY; POLYAMINES; SURFACE PROPERTIES; TIME FACTORS;

EID: 0142042448     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026166729873     Document Type: Article

References (31)

1. D. A. Tomalia. Dendrimer molecules. Sci. Am. 272:62-66 (1995).
2. A. D'Emanuele, D. Attwood, and R. Abu-Rmaileh. Dendrimers. In J. Swarbrick and J. C. Boylan (eds.), Encyclopedia of Pharmaceutical Technology, 2nd ed., update 3. Marcel Dekker, New York, 2002, pp. 1-21.
3. J. C. Roberts, M. K. Bhalgat, and R. T. Zera. Preliminary biological evaluation of polyamidoamine (PAMAM) Starburst dendrimers. J. Biomed. Mater. Res. 30:53-65 (1996).
4. 125I-labelled polyamidoamine dendrimers in vivo. J. Contr. Rel. 65: 133-148 (2000).
5. R. Jevprasesphant, J. Penny, R. Jalal, D. Attwood, N. B. McKeown, and A. D'Emanuele. The influence of surface modification on the cytotoxicity of PAMAM dendrimers. Int. J. Pharm. 252:263-266 (2003).
6. A. T. Florence, T. Sakthivel, and I. Toth. Oral uptake and translocation of a polylysine dendrimer with a lipid surface. J. Contr. Rel. 65:253-259 (2000).
7. R. Wiwattanapatapee, B. Carreno-Gomez, N. Malik, and R. Duncan. Anionic PAMAM dendrimers rapidly cross adult rat intestine in vitro: a potential oral delivery system. Pharm. Res. 17:991-998 (2000).
8. M. El-Sayed, M. Ginski, C. Rhodes, and H. Ghandehari. Transepithelial transport of poly(amidoamine) dendrimers across Caco-2 cell monolayers. J. Contr. Rel. 81:355-365 (2002).
9. P. Artursson. Epithelial transport of drugs in cell culture. I: A model for studying the passive diffusion of drugs over intestinal absorptive (Caco-2) cells. J. Pharm. Sci. 79:476-482 (1990).
10. P. Artursson and R. T. Borchardt. Intestinal drug absorption and metabolism in cell cultures, Caco-2 and beyond. Pharm. Res. 14: 1655-1658 (1997).
11. R. T. Borchardt, H. E. Lane, B. H. Hirst, P. L. Smith, K. L. Audus, and A. Tsuji. Application of cell culture systems to the study of drug transport and metabolism. In A. C. Cuello and B. Collier (eds.), Pharmacological Sciences: Perspectives for Research and Therapy in the Late 1990s, Verlag, Basel, 1995, pp. 493-501.
12. C. A. Bailey, P. Bryla, and A. W. Malick. The use of the intestinal epithelial cell culture model, Caco-2, in pharmaceutical development. Adv. Drug Deliv. Rev. 22:85-103 (1996).
13. L.-S. L. Gan and D. R. Thakker. Applications of the Caco-2 model in the design and development of orally active drugs: elucidation of biochemical and physical barriers posed by the intestinal epithelium. Adv. Drug Deliv. Rev. 23:77-98 (1997).
14. P. Artursson, K. Palm, and K. Luthman. Caco-2 monolayers in experimental and theoretical predictions of drug transport. Adv. Drug Deliv. Rev. 46:27-23 (2001).
15. T. Lindmark, T. Nikkila, and P. Artursson. Mechanisms of absorption enhancement by medium chain fatty acids in intestinal epithelial Caco-2 cell monolayers. J. Pharmacol. Exp. Ther. 275: 958-964 (1995).
16. K. Higaki, T. Yata, M. Sone, K.-I. Ogawara, and T. Kimura. Estimation of absorption enhancement by medium-chain fatty acids in rat large intestine. Res. Commun. Mol. Pathol. Pharmacol. 109:231-240 (2001).
17. Y. Kimura, Y. Hosoda, M. Shima, S. Adachi, and R. Matsuno. Physicochemical properties of fatty acids for assessing the threshold concentration to enhance the absorption of a hydrophilic substance. Biosci. Biotechnol. Biochem. 62:443-447 (1998).
18. T. Lindmark, Y. Kimura, and P. Artursson. Absorption enhancement through intracellular regulation of tight junction permeability by medium chain fatty acids in Caco-2 cells. J. Pharmacol. Exp. Ther. 284:362-369 (1998).
19. M. Tomita, M. Hayashi, and S. Awazu. Absorption-enhancing mechanism of EDTA, caprate, and decanoylcarnitine in Caco-2 cells. J. Pharm. Sci. 85:608-611 (1996).
20. H. S. Thatte, K. R. Bridges, and D. E. Golan. Microtubule inhibitors differentially affect translational movement, cell surface expression, and endocytosis of transferrin receptors in K562 cells. J. Cell. Physiol. 160:345-357 (1994).
21. S. L. Snyder and P. Z. Sobocinski. An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines. Anal. Biochem. 64:284-288 (1975).
22. T. Mosmann. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immun. Methods 65: 55-63 (1983).
23. G. A. Brazeau, S. Attia, S. Poxon, and J. A. Hughes. In vitro myotoxicity of selected cationic macromolecules used in non-viral gene delivery. Pharm. Res. 15:680-684 (1998).
24. H. Yoo and R. L. Juliano. Enhanced delivery of antisense oligonucleotides with fluorophore-conjugated PAMAM dendrimers. Nucleic Acids Res. 28:4225-4231 (2000).
25. V. H. L. Lee and J. J. Yang. Oral Drug Delivery. In A. M. Hillery, A. W. Lloyd, and J. Swarbrick (eds.). Drug Delivery and Targeting, Taylor and Francis, London, 2001, pp. 145-183.
26. P. Artursson, T. Lindmark, S. S. Davis, and L. Illum. Effect of chitosan on the permeability of monolayers of intestinal epithelial cells (Caco-2). Pharm. Res. 11:1358-1361 (1994).
27. G. Ranaldi, I. Marigliano, I. Vespignani, G. Perozzi, and Y. Sambuy. The effect of chitosan and other polycations on tight junction permeability in the human intestinal Caco-2 cell line. J. Nutr. Biochem. 13:157-167 (2002).
28. P. Anderle, E. Niederer, W. Rubas, C. Hilgendorf, H. Spahn-Langguth, H. Wunderli-Allenspach, H. P. Merkle, and P. Langguth. P-glycoprotein (P-gp) mediated efflux in Caco-2 cell monolayers: the influence of culturing conditions and drug exposure on P-gp. J. Pharm. Sci. 87:757-762 (1998).
29. C. H. M. Versantvoort, R. C. A. Ondrewater, E. Duizer, J. J. M. Van de Sandt, A. J. Gilde, and J. P. Groten. Monolayers of IEC-18 cells as an in vitro model for screening the passive transcellular and paracellular transport across the intestinal barrier: comparison of active and passive transport with the human colon carcinoma Caco-2 cell line. Environ. Toxicol. Pharmacol. 11:335-344 (2002).
30. A. B. J. Noach, Y. Kurosaki, M. C. M. Blom-Roosemalen, A. G. de Boer, and D. D. Breimer. Cell-polarity dependent effect of chelation on the paracellular permeability of confluent Caco-2 cell monolayers. Int. J. Pharm. 90:229-237 (1993).
31. 2+ in cultured epithelia. Biochim. Biophys. Acta 1222:147-158 (1994).