Inhibition of gene expression in Escherichia coli by antisense phosphorodiamidate morpholino oligomers

Antimicrobial Agents and Chemotherapy

Volumn 47, Issue 10, 2003, Pages 3233-3239

  Geller, B L ^a,b   Deere, J D ^b   Stein, D A ^b   Kroeker, A D ^b   Moulton, H M ^b   Iversen, P L ^b  

^a OREGON STATE UNIVERSITY   (United States)

^b AVI BIOPHARMA INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL CARRIER PROTEIN; ANTISENSE OLIGONUCLEOTIDE; ANTISENSE PHOSPHORODIAMIDATE MORPHOLINO OLIGOMER; BACTERIAL PROTEIN; BETA GALACTOSIDASE; CARRIER PROTEIN; CYSTEINYLARGINYLARGINYLARGINYLGLUTAMINYLLYSYLLYSYLLYSYLARGININE; ESCHERICHIA COLI PROTEIN; GENE PRODUCT; LACI PROTEIN, BACTERIA; LUCIFERASE; LYSYLPHENYLALANYLCYSTEINYLLYSYLPHENYLALANYLCYSTEINYLLYSYLPHENYLALANYLCYSTEI NYLLYSYLCYSTEINE; MESSENGER RNA; MORPHOLINE DERIVATIVE; OLIGOMER; PHOSPHORODIAMIDATE MORPHOLINO OLIGOMER; POLYPEPTIDE; PROTEIN ACPP; PROTEIN LACI; REPRESSOR PROTEIN; RNA 16S; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL OUTER MEMBRANE; BACTERIAL VIRULENCE; BACTERIUM IDENTIFICATION; CELL MEMBRANE PERMEABILITY; CELL MEMBRANE TRANSPORT; CHEMISTRY; CONCENTRATION RESPONSE; CONJUGATION; CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE INACTIVATION; GENETICS; METABOLISM; NONHUMAN; NUCLEOTIDE SEQUENCE; ONCOGENE MYC; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REPORTER GENE;

ACYL CARRIER PROTEIN; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; CELL MEMBRANE PERMEABILITY; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENES, MYC; GENES, REPORTER; LUCIFERASES; MEMBRANE TRANSPORT PROTEINS; MORPHOLINES; OLIGONUCLEOTIDES, ANTISENSE; REPRESSOR PROTEINS; RNA, RIBOSOMAL, 16S;

EID: 0141890298     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.47.10.3233-3239.2003     Document Type: Article

References (28)

1. Arora, V., D. C. Knapp, B. L. Smith, M. L. Statdfield, D. A. Stein, M. T. Reddy, D. D. Weller, and P. L. Iversen. 2000. c-Myc antisense limits rat liver regeneration and indicates role for c-Myc in regulating cytochrome P-450 3A activity. J. Pharmacol. Exp. Ther. 292:921-928.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, K. Struhl. 1998. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
3. Borer, P. N. 1975. Optical properties of nucleic acids, adsorption, and circular dichroism spectra, p. 589. In G. D. Fasman (ed.). Handbook of biochemistry and molecular biology, 3rd ed., vol. 1. Nucleic acids. CRC Press, Cleveland, Ohio.
4. Eriksson, M., P. E. Nielsen, and L. Good. 2002. Cell permeabilization and uptake of antisense peptide-peptide nucleic acid (PNA) into Escherichia coli. J. Biol. Chem. 277:7144-7147.
5. Fawell, S., J. Seery, Y. Daikh, C. Moore, L. L. Chen, B. Pepinsky, and J. Barsoum. 1994. Tat-mediated delivery of heterologous proteins into cells. Proc. Natl. Acad. Sci. USA 91:664-668.
6. Galloway, S., and C. R. H. Raetz. 1990. A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis. J. Biol. Chem. 265:6394-6402.
7. Good, L., S. K. Awasthi, R. Dryselius, O. Larsson, and P. E. Nielsen. 2001. Bacterial antisense effects of peptide-PNA conjugates. Nat. Biotechnol. 19:360-364.
8. Good, L., and P. E. Nielsen. 1998. Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA. Nat. Biotechnol. 16:355-358.
9. Good, L., and P. E. Nielsen. 1998. Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA. Proc. Natl. Acad. Sci. USA 95:2073-2076.
10. Good, L., R. Sandberg, O. Larsson, P. E. Nielsen, and C. Wahlestedt. 2000. Antisense PNA effects in Escherichia coli are limited by the outer membrane LPS layer. Microbiology 146:2665-2670.
11. Hudziak, R., L. Barofsky, D. Barofsky, D. L. Weller, S.-B. Huang, and D. D. Weller. 1996. Resistance of morpholino phosphorodiamidate oligomers to enzymatic degradation. Antisense Nucleic Acid Drug Dev. 6:267-272.
12. Hwang, P. M., and H. J. Vogel. 1998. Structure-function relationships of antimicrobial peptides. Biochem. Cell Biol. 76:235-246.
13. Miller, J. H. 1972. Experiments in molecular biology. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
14. Moulton, H. M., M. C. Hase, C. M. Smith, and P. L. Iversen. 2003. HIV Tat peptide enhances cellular delivery of antisense morpholino oligomers. Antisense Nucleic Acid Drug Dev. 13:31-43.
15. Nikaido, H. 1993. Transport across the bacterial outer membrane. J. Bioenerg. Biomembr. 25:581-589.
16. Nikaido, H., and M. Vaara. 1997. Outer membrane, p. 7-22. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology. American Society for Microbiology. Washington, D.C.
17. Qin, G., M. Taylor, Y. Y. Ning, P. Iversen, and L. Kobzik. 2000. In vivo evaluation of a morpholino antisense oligomer directed against tumor necrosis factor-α. Antisense Nucleic Acid Drug Dev. 10:11-16.
18. Rustici, A., M. Velucchi, R. Faggioni, M. Sironi, P. Ghezzi, S. Quataert, B. Green, and M. Porro. 1993. Molecular mapping and detoxification of the lipid A binding site by synthetic peptides. Science 259:361-365.
19. Schwarze, S. R., A. Ho, A. Vocero-Akbani, and S. F. Dowdy. 1999. In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 285:1569-1572.
20. Shai, Y. 1999. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by α-helical antimicrobial and cell non-selective membrane peptides. Biochim. Biophys. Acta 1462:55-70.
21. Stein, D., E. Foster, S.-B. Huang, D. Weller, and J. Summerton. 1997. A specificity comparison of four antisense types: morpholino, 2′-O-methyl RNA, DNA, and phosphorothioate DNA. Antisense Nucleic Acid Drug Dev. 7:151-157.
22. Summerton, J., D. Stein, S. B. Huang, P. Mathews, D. Weller, and M. Partridge. 1997. Morpholino and phosphorothioate antisense oligomers compared in cell-free and in-cell systems. Antisense Nucleic Acid Drug Dev. 7:63-70.
23. Summerton, J., and D. Weller. February 1993. Uncharged morpholino-based polymers having phosphorus containing chiral inter-subunit linkages. U.S. patent 5,185,444.
24. Summerton, J., and D. Weller. 1997. Morpholino antisense oligomers: design, preparation, and properties. Antisense Nucleic Acid Drug Dev. 7:187-195.
25. Vaara, M., and M. Porro. 1996. Groups of peptides that act synergistically with hydrophobic antibiotics against gram-negative enteric bacteria. Antimicrob. Agents Chemother. 40:1801-1805.
26. Vuorio, R., and M. Vaara. 1992. The lipid A biosynthesis mutant lpxA2 of Escherichia coli results in drastic antibiotic supersusceptibility. Antimicrob. Agents Chemother. 36:826-829.
27. Wetlaufer, D. B. 1962. Ultraviolet spectra of proteins and amino acids. Adv. Protein Chem. 17:303-390.
28. Zasloff, M. 2002. Antimicrobial peptides of multicellular organisms. Nature 415:389-395.