Co-overexpression of Escherichia coli RNA polymerase subunits allows isolation and analysis of mutant enzymes lacking lineage-specific sequence insertions

Journal of Biological Chemistry

Volumn 278, Issue 14, 2003, Pages 12344-12355

  Artsimovitch, Irina ^a   Svetlov, Vladimir ^a   Murakami, Katsuhiko S ^b   Landick, Robert ^c  

^a OHIO STATE UNIVERSITY   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; GENES; MUTAGENESIS; RNA;

GENE EXPRESSION;

ENZYMES;

ENZYME; MUTANT ENZYME; PROTEIN SUBUNIT; RNA POLYMERASE; UNCLASSIFIED DRUG; BETA' SUBUNIT OF RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; RNA POLYMERASE ALPHA SUBUNIT; RNA POLYMERASE BETA SUBUNIT;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; CELL GROWTH; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE OVEREXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN FOLDING; SEQUENCE INSERTION; TRANSCRIPTION REGULATION; CHEMISTRY; ENZYMOLOGY; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; IN VITRO STUDY; PLASMID; PROTEIN TERTIARY STRUCTURE; TRANSPOSON;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BACILLUS SUBTILIS; DNA TRANSPOSABLE ELEMENTS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; PLASMIDS; PROTEIN STRUCTURE, TERTIARY;

EID: 0038823636     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M211214200     Document Type: Article

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