A mutation in the C-terminal domain of the RNA polymerase alpha subunit that destabilizes the open complexes formed at the phage φ29 late A3 promoter

Journal of Molecular Biology

Volumn 307, Issue 2, 2001, Pages 487-497

  Calles, Belén ^a   Monsalve, María ^a   Rojo, Fernando ^b   Salas, Margarita ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Bacterial promoters; Protein DNA interactions; RNA polymerase subunit; Transcription initiation; Transcription regulation

Indexed keywords

DNA FRAGMENT; RNA POLYMERASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIOPHAGE; COMPLEX FORMATION; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA INTERACTION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POUTERIA SANDWICENSIS;

EID: 0035937254     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4511     Document Type: Article

References (49)

1. Compilation and analysis of Escherichia coli promoter DNA sequences
2. A third recognition element in bacterial promoters: DNA binding by the α subunit of RNA polymerase
3. Polypeptides containing highly conserved regions of transcription initiation sigma factor 70 exhibit specific binding to promoter DNA
4. Domain organization of RNA polymerase α subunit: C-terminal 85 amino acids constitute a domain capable of dimerization and DNA binding
5. 70) promoters, and the kinetics of the steps of transcription initiation
6. Structural map of the alpha subunit of Escherichia coli RNA polymerase: Structural domains identified by proteolytic cleavage
7. The Escherichia coli RNA polymerase α subunit linker: Length requirements for transcription activation at CRP-dependent promoters
8. Subunit assembly of Escherichia coli RNA polymerase
9. Transcription activation at class II CAP-dependent promoters: Two interactions between CAP and RNA polymerase
10. Protein-protein communication within the transcriptional apparatus
11. The Escherichia coli RNA polymerase α subunit: Structure and function
12. Repression of transcription initiation in bacteria
13. Factor independent activation of rrnB P1. An "extended" promoter with an upstream element that dramatically increases promoter strength
14. Protein p4 represses phage φ29 A2c promoter by interacting with the α subunit of Bacillus subtilis RNA polymerase
15. Transcription activation or repression by phage φ29 protein p4 depends on the strength of the RNA polymerase-promoter interactions
16. Residues of the Bacillus subtilis phage φ29 transcriptional activator required both to interact with RNA polymerase and to activate transcription
17. Transcription activation by phage φ29 protein p4 is mediated by interaction with the α subunit of Bacillus subtilis RNA polymerase
18. Transcriptional activator of phage φ29 late promoter: Mapping of residues involved in interaction with RNA polymerase and in DNA bending
19. Activation and repression of transcription at two different phage φ29 promoters are mediated by interaction of the same residues of regulatory protein p4 with the RNA polymerase
20. Database of homology-derived protein structures and the structural meaning of sequence alignment
21. Solution structure of the activator contact domain of the RNA polymerase α subunit
22. A DNA curvature can substitute phage φ29 regulatory protein p4 when acting as a transcriptional repressor
23. Phage φ29 regulatory protein p4 stabilizes the binding of the RNA polymerase to the late promoter in a process involving direct protein-protein contacts
24. A-RNA polymerase that are stabilized by DNA supercoiling
25. RM promoter are sequence nonspecific and activate promoter to a lesser extent than an introduced UP element of an rRNA promoter
26. Promoter recognition by Escherichia coli RNA polymerase: Effects of the UP element on open complex formation and promoter clearance
27. Bacterial promoter architecture: Subsite structure of UP elements and interactions with the carboxy-terminal domain of the RNA polymerase α subunit
28. DNA-binding determinants of the α subunit of RNA polymerase: Novel DNA-binding domain architecture
29. Transcription activation by catabolite activator protein (CAP)
30. Bacterial sigma factors
31. A promoter melting region in the primary σ factor of Bacillus subtilis. Identification of functionally important aromatic amino acids
32. UPs and downs in bacterial transcription initiation: The role of the alpha subunit of RNA polymerase in promoter recognition
33. The tyrosine tRNA promoter
34. Visualization and quantitative analysis of complex formation between E. coli RNA polymerase and an rRNA promoter in vitro
35. Factor independent activation of rRNA transcription. I. Kinetic analysis of the roles of the upstream activator region and supercoiling on the rrnB P1 promoter in vitro
36. Mechanism of initiation of transcription by Bacillus subtilis RNA polymerase at several promoters
37. Mutant σ factor blocks transition between promoter binding and initiation of transcription
38. 70 family: Sequence conservation and evolutionary relationships
39. 70 is required for efficient isomerization and initiation of transcription by Escherichia coli RNA polymerase
40. RNA polymerase mutants that destabilize RNA polymerase-promoter complexes alter NTP-sensing by rrn P1 promoters
41. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system
42. Suppressor-sensitive mutants and genetic map of Bacillus subtilis bacteriophage φ29
43. Physical map of bacteriophage φ29 DNA
44. A general rapid mutagenesis method using polymerase chain reaction
45. RNA polymerase binding sites and transcription of the DNA of Bacillus subtilis phage φ29
46. Transcription regulation in bacteriophage φ29: Expression of the viral promoters throughout the infection cycle
47. Location, structure and function of the target of a transcriptional activator protein
48. Transcription activation at class II CRP-dependent promoters: Identification of determinants in the C-terminal domain of the RNA polymerase α subunit