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The label Ec preceding amino acid numbers indicates E. coli numbering, Ta indicates Taq numbering. If no indication is given, E. coli numbering is assumed.
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The evolutionarily conserved segments of the large β′ and β subunits (regions A to H and A to I, respectively) have been defined previously (1, 6).
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Figures 3 and 4 were made with the program GRASP [A. Nicholls, K. A. Sharp, B. Honig, Prot. Struct. Funct. Genet. 11, 281 (1991)]. We thank K. Severinov for the strain overproducing the Xho 19 β subunit mutant and M. Chlenov for participation in development of the DNA-protein cross-linking methods. Supported in part by NIH grants GM30717 and GM49242 (to A.G.) and NIH grant GM53759 (to S.A.D.).
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