Inhibition of staurosporine-induced apoptosis of endothelial cells by activated protein C requires protease-activated receptor-1 and endothelial cell protein C receptor

Biochemical Journal

Volumn 373, Issue 1, 2003, Pages 65-70

  Mosnier, Laurent O ^a   Griffin, John H ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Activated protein C; Apoptosis; Endothelial cell protein C receptor (EPCR); Protease activated receptor (PAR)

Indexed keywords

CELLS; ENZYMES; PROTEINS;

PROTEOLYSIS;

BIOCHEMISTRY;

ACTIVATED PROTEIN C; ENDOTHELIAL CELL PROTEIN C RECEPTOR; PROTEINASE ACTIVATED RECEPTOR 1; RECEPTOR; STAUROSPORINE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL LINE; DOSE TIME EFFECT RELATION; ENDOTHELIUM CELL; ENZYME ACTIVATION; MODEL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

ANIMALS; ANTICOAGULANTS; APOPTOSIS; BLOOD COAGULATION FACTORS; CELL LINE; ENDOTHELIUM, VASCULAR; ENZYME INHIBITORS; KINETICS; MODELS, BIOLOGICAL; PROTEIN KINASE C; RECEPTOR, PAR-1; RECEPTORS, CELL SURFACE; RECEPTORS, THROMBIN; STAUROSPORINE;

EID: 0038682435     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030341     Document Type: Article

References (26)

1. Esmon, C. T. (2000) Regulation of blood coagulation. Biochim. Biophys. Acta 1477, 349-360
2. Fuentes-Prior, P., Iwanaga, Y., Huber, R., Pagila, R., Rumennik, G., Seto, M., Morser, J., Light, D. R. and Bode, W. (2000) Structural basis for the anticoagulant activity of the thrombin-thrombomodulin complex. Nature (London) 404, 518-525
3. Griffin, J. H., Zlokovic, B. V. and Fernandez, J. A. (2002) Activated protein C: potential therapy for severe sepsis, thrombosis and stroke. Semin. Hematol. 39, 197-205
4. Conway, E. M., Van de Wouwer, M., Pollefeyt, S., Jurk, K., Van Aken, H., De Vriese, A., Weitz, J. I., Weiler, H., Hellings, P. W., Schaeffer, P. et al. (2002) The lectin-like domain of thrombomodulin confers protection from neutrophil-mediated tissue damage by suppressing adhesion molecule expression via nuclear factor kappaB and mitogen-activated protein kinase pathways. J. Exp. Med. 196, 565-577
5. Rothbarth, K., Dabaghian, A. R. H., Stammer, H. and Werner, D. (1999) One single mRNA encodes the centrosomal protein COD41 and the endothelial cell protein C receptor (EPCR). FEBS Lett. 458, 77-80
6. Fukudome, K. and Esmon, C. T. (1995) Molecular cloning and expression of routine and bovine endothelial cell protein C/activated protein C receptor (EPCR). The structural and functional conservation in human, bovine, and murine EPCR. J. Biol. Chem. 270, 5571-5577
7. Oganesyan, V., Oganesyan, N., Terzyan, S., Qu, D., Dauter, Z., Esmon, N. L. and Esmon, C. T. (2002) The crystal structure of the endothelial protein C receptor and a bound phospholipid. J. Biol. Chem. 277, 24851-24854
8. Shu, F., Kobayashi, H., Fukudome, K., Tsuneyoshi, N., Kimoto, M. and Terao, T. (2000) Activated protein C suppresses tissue factor expression on U937 cells in the endothelial protein C receptor-dependent manner. FEBS Lett. 477, 208-212
9. Isobe, H., Okajima, K., Uchiba, M., Mizutani, A., Harada, N., Nagasaki, A. and Okabe, K. (2001) Activated protein C prevents endotoxin-induced hypotension in rats by inhibiting excessive production of nitric oxide. Circulation 104, 1171-1175
10. Esmon, C. T. (2002) Protein C pathway in sepsis. Ann. Med. 34, 598-605
11. Taylor, Jr, F. B., Stearns-Kurosawa, D. J., Kurosawa, S., Ferrell, G, Chang, A. C., Laszik, Z., Kosanke, S., Peer, G. and Esmon, C. T. (2000) The endothelial cell protein C receptor aids in host defense against Escherichia coli sepsis. Blood 95, 1680-1686
12. Joyce, D. E., Gelbert, L., Ciaccia, A., DeHoff, B. and Grinnell, B. W. (2001) Gene expression profile of antithrombotic protein C defines new mechanisms modulating inflammation and apoptosis. J. Biol. Chem. 276, 11199-11203
13. Bernard, G. R., Vincent, J. L., Laterre, P. F., LaRosa, S. P., Dhainaut, J. F., Lopez-Rodriguez, A., Steingrub, J. S., Garber, G. E., Helterbrand, J. D., Ely, E. W. and Fisher, Jr, C. J. (2001) Efficacy and safety of recombinant human activated protein C for severe sepsis. N. Engl. J. Med. 344, 699-709
14. Warren, B. L., Eid, A., Singer, P., Pillay, S. S., Carl, P., Novak, I., Chalupa, P., Atherstone, A., Penzes, I., Kubler, A. et al. (2001) Caring for the critically ill patient. High-dose antithrombin III in severe sepsis. A randomized controlled trial J. Am. Med. Assoc. 286, 1869-1878
15. Abraham, E., Reinhart, K., Svoboda, P., Seibert, A., Olthoff, D., Dal Nogare, A., Postier, R., Hempelmann, G., Butler, T., Martin, E. et al. (2001) Assessment of the safety of recombinant tissue factor pathway inhibitor in patients with severe sepsis: a multicenter, randomized, placebo-controlled, single-blind, dose escalation study. Crit. Care Med. 29, 2081-2089
16. Riewald, M., Petrovan, R. J., Donner, A., Mueller, B. M. and Ruf, W. (2002) Activation of endothelial cell protease activated receptor 1 by the protein C pathway. Science 296, 1880-1882
17. Cheng, T., Liu, D., Griffin, J. H., Fernandez, J. A., Castellino, F. J., Rosen, E. D., Fukudome, K. and Zlokovic, B. V. (2003) Activated protein C blocks P53-mediated apoptosis in ischemic human brain endothelium and is neuroprotective in a murine stroke model through EPCR and PAR-1. Nat. Med. 9, 338-342
18. Heeb, M. J., Schwarz, H. P., White, T., Lammle, B., Berrettini, M. and Griffin, J. H. (1988) Immunoblotting studies of the molecular forms of protein C in plasma. Thromb. Res. 52, 33-43
19. Gale, A. J., Sun, X., Heeb, M. J. and Griffin, J. H. (1997) Nonenzymatic anticoagulant activity of the mutant serine protease Ser360Ala-activated protein C mediated by factor Va. Protein Sci. 6, 132-140
20. Edgell, C. J. S., McDonald, C. C. and Graham, J. B. (1983) Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. U.S.A. 80, 3734-3737
21. Idziorek, T., Estaquier, J., De Bels, F. and Ameisen, J. C. (1995) YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability J. Immunol. Methods 185, 249-258
22. Kulioputos, A., Covic, L., Seeley, S. K., Sheridan, P. J., Helin, J. and Costello, C. E. (1999) Plasmin desensitization of the PAR1 thrombin receptor: kinetics, sites of truncation, and implications for thrombolytic therapy. Biochemistry 38, 4572-4585
23. Coughlin, S. R. (2000) Thrombin signaling and protease activated receptors. Nature (London) 407, 258-264
24. Stearns-Kurosawa, D. J., Kurosawa, S., Mollica, J. S., Ferrell, G. L. and Esmon, C. T. (1996) The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex. Proc. Natl. Acad. Sci. U.S.A. 93, 10212-10216
25. Regan, L. M., Stearns-Kurosawa, D. J., Kurosawa, S., Mollica, J., Fukudome, K. and Esmon, C. T. (1996) The endothelial cell protein C receptor. Inhibition of activated protein C anticoagulant function without modulation of reaction with proteinase inhibitors. J. Biol. Chem. 271, 17499-17803
26. Esmon, C. T. (2000) The endothelial protein C receptor. Thromb. Haemostasis 83, 639-643