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The affinity of P3 was determined by isothermal titration microcalorimetry, as it contains no tryptophan fluorophores.
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222 as described in ref 32, the number of contributing residues is reduced to 16 of 33, or 48%.
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"Free metal ions" describe ions not complexed by peptide, although they exist as aquo/hydroxide Tris-stabilized species under these conditions.
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43
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32P- labeled oligonucleotides.
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45
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note
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3 in both directions (paralleling the Ce(IV) results), the data are inconclusive.
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46
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0037691321
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note
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No change in background occurs during the course of the reaction, as 0 h and 24 h control lanes look identical.
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47
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