Use of RNA interference to investigate gene function in the human filarial nematode parasite Brugia malayi

Molecular and Biochemical Parasitology

Volumn 129, Issue 1, 2003, Pages 41-51

  Aboobaker, A Aziz ^a   Blaxter, Mark L ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Brugia malayi; Caenorhabditis elegans; Filarial nematode; Gene function; Phenotype; RNAi

Indexed keywords

BETA TUBULIN; BRUGIA MALAYI SHEATH PROTEIN 1; DOUBLE STRANDED DNA; HELMINTH PROTEIN; PROTEIN; PROTEIN SUBUNIT; RNA POLYMERASE II; UNCLASSIFIED DRUG;

ANALYSIS; ARTICLE; BRUGIA MALAYI; CONTROLLED STUDY; CULTURE TECHNIQUE; FEMALE; FILARIA; FLUORESCENCE; GENE; GENE FUNCTION; GENE TARGETING; GENETIC PROCEDURES; HOUSEKEEPING GENE; LETHAL GENE; LIFESPAN; MICROFILARIA; MOLECULE; NEMATODE; NONHUMAN; PARASITE; PARASITE CULTIVATION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN TARGETING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION;

BRUGIA MALAYI; CAENORHABDITIS ELEGANS;

EID: 0038512548     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(03)00092-6     Document Type: Article

References (49)

1. Riddle D, Blumenthal T, Meyer B, Priess J, editors. C. elegans II. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1997.
2. The C. elegans Sequencing Consortium. Genome sequence of the nematode C. elegans: a platform for investigating biology. Science 1998;282:2012-18.
3. Ahringer J. Turn to the worm! Curr. Opin. Genet. Dev. 7:1997;410-415.
4. Aboobaker A.A., Blaxter M.L. Medical significance of Caenorhabditis elegans. Ann. Med. 32:2000;23-30.
5. Mello C.C., Kramer J.C., Stinchcomb D., Ambros V. Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 10:1991;3959-3970.
6. Plasterk R.H.A., Groenen J.T.M. Targeted alterations of the Caenorhabditis elegans genome by transgene instructed DNA double strand break repair following Tc1 excision. EMBO J. 11:1992;287-290.
7. Parkinson J., Whitton C., Guiliano D., Daub J., Blaxter M.L. 200,000 nematode ESTs on the net. Trends Parasitol. 17:2001;394-396.
8. Williams S.A., Lizotte-Waniewski M.R., Foster J.et al. The filarial genome project: analysis of the nuclear, mitochondrial and endosymbiont genomes of Brugia malayi. Int. J. Parasitol. 30:2000;411-419.
9. Blaxter M., Daub J., Guiliano D.et al. The Brugia malayi genome project: expressed sequence tags and gene discovery. Trans. R. Soc. Trop. Med. Hyg. 96:2001;1-11.
10. Blaxter M, Whitton C, Thompson M, et al. Comparative nematode genomics. Nematology, in press.
11. Fire A. RNA-triggered gene silencing. Trends Genet. 15:1999;358-363.
12. Kuwabara P.E., Coulson A. RNAi-prospects for a general technique for determining gene function. Parasitol. Today. 16:2000;347-349.
13. Fire A., Xu S., Montgomery M.K.et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:1998;806-811.
14. Al N.C., Lm L.P., Winston E.G., Barrel D.P. An abundant class of tiny RAS with probable regulatory roles in Caenorhabditis elegans. Science. 294:2001;858-862.
15. Lee R.C., Ambros V. An extensive class of small RAs in Caenorhabditis elegans. Science. 294:2001;862-864.
16. Kettering R.F., Haverkamp T.H., van Luenen H.G., Plasterk R.H. mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell. 99:1999;133-141.
17. Tabara H., Grishok A., Mello C.C. RNAi in C. elegans: soaking in the genome sequence. Science. 282:1998;430-431.
18. Timmons L., Fire A. Specific interference by ingested dsRNA. Nature. 395:1998;854.
19. Zamore P.D. RNA interference: listening to the sound of silence. Nat. Struct. Biol. 8:2001;746-750.
20. Hussein A.S., Kichenin K., Selkirk M.E. Suppression of secreted acetylcholinesterase expression in Nippostrongylus brasiliensis by RNA interference. Mol. Biochem. Parasitol. 122:2002;91-94.
21. Chen S.N., Howells R.E. Brugia pahangi: uptake and incorporation of nucleic acid precursors by microfilariae and macrofilariae in vitro. Exp. Parasitol. 51:1981;296-306.
22. Howells R.E., Chen S.N. Brugia pahangi: feeding and nutrient uptake in vitro. Exp. Parasitol. 51:1981;42-58.
23. Howells R.E. The mechanisms of amino acid uptake by Brugia pahangi in vitro. Z. Parasitenkd. 69:1983;247-253.
24. Bird D.M., Riddle D.L. Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II. Mol. Cell Biol. 9:1989;4119-4130.
25. Sidow A., Thomas W.K. A molecular evolutionary framework for eukaryotic model organisms. Curr. Biol. 4:1994;596-603.
26. Helm R., Selkirk M.E., Bradley J.E.et al. Localisation and immunogenicity of tubulin in the filarial nematodes Brugia malayi and B. pahangi. Parasite Immunol. 11:1989;479-502.
27. Selkirk M.E., Yazdanbakhsh M., Freedman D.et al. A proline-rich structural protein of the surface sheath of larval Brugia filarial nematode parasites. J. Biol. Chem. 266:1991;11002-11008.
28. Christ H., Hirzmann J., Conraths F.et al. Trans-splicing of an early embryo mRNA in Litomosoides carinii, coding for the major microfilarial sheath protein gp22. Gene. 121:1992;219-226.
29. Devaney E. Lectin-binding characteristics of Brugia pahangi microfilariae. Tropenmed. Parasitol. 36:1985;25-28.
30. Maeda I., Kohara Y., Yamamoto M., Sugimoto A. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr. Biol. 11:2001;171-176.
31. Blaxter M.L., Raghavan N., Ghosh I.et al. Genes expressed in Brugia malayi infective third stage larvae. Mol. Biochem. Parasitol. 77:1996;77-96.
32. Prichard R. Anthelmintic resistance. Vet. Parasitol. 54:1994;259-268.
33. Zahner H., Hobom G., Stirm S. The microfilarial sheath and its proteins. Parasitol. Today. 11:1995;116-120.
34. Hirzmann J., Schnaufer A., Hintz M.et al. Brugia spp. and Litomosoides carinii: identification of a covalently cross-linked microfilarial sheath matrix protein (shp2). Mol. Biochem. Parasitol. 70:1995;95-106.
35. Conraths F.J., Schützle B., Schares G.et al. The gene coding for the major sheath protein of Litomosoides carinii microfilariae, gp22, is transcribed in oocytes and embryonic cells. Mol. Biochem. Parasitol. 60:1993;111-120.
36. Dillin A., Hsu A.L., Arantes-Oliveira N.et al. Rates of behavior and aging specified by mitochondrial function during development. Science. 298:2002;2398-2401.
37. Kuwabara P.E. Worming your way through the genome. Trends Genet. 13:1997;455-460.
38. Urwin P.E., Lilley C.J., Atkinson H.J. Ingestion of double-stranded RNA by preparasitic juvenile cyst nematodes leads to RNA interference. Mol. Plant Microbe. Interact. 15:2002;747-752.
39. Falcone F.H., Loke P., Zang X.et al. A Brugia malayi homolog of macrophage migration inhibitory factor reveals an important link between macrophages and eosinophil recruitment during nematode infection. J. Immunol. 167:2001;5348-5354.
40. Zang X., Taylor P., Wang J.M.et al. Homologues of human macrophage migration inhibitory factor from a parasitic nematode. Gene cloning, protein activity, and crystal structure. J. Biol. Chem. 277:2002;44261-44267.
41. Maizels R.M., Gomez-Escobar N., Gregory W.F., Murray J., Zang X. Immune evasion genes from filarial nematodes. Int. J. Parasitol. 31:2001;889-898.
42. Maizels R.M., Blaxter M.L., Scott A.L. Immunological genomics of Brugia malayi: filarial genes implicated in immune evasion and protective immunity. Parasite Immunol. 23:2001;327-344.
43. Kamath R.S., Fraser A.G., Dong Y.et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature. 421:2003;231-237.
44. Lee S.S., Lee R.Y., Fraser A.G.et al. A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nat. Genet. 33:2003;40-48.
45. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, Ahringer J. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol 2001;2(1):RESEARCH0002.
46. Fraser A.G., Kamath R.S., Zipperlen P.et al. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature. 408:2000;325-330.
47. Hashmi S., Ling P., Hashmi G.et al. Genetic transformation of nematodes using arrays of micromechanical piercing structures. Biol. Technol. 19:1995;766-770.
48. Higazi T.B., Merriweather A., Shu L., Davis R., Unnasch T.R. Brugia malayi: transient transfection by microinjection and particle bombardment. Exp. Parasitol. 100:2002;95-102.
49. Jackstadt P., Wilm T.P., Zahner H., Hobom G. Transformation of nematodes via ballistic DNA transfer. Mol. Biochem. Parasitol. 103:1999;261-266.