Suppression of secreted acetylcholinesterase expression in Nippostrongylus brasiliensis by RNA interference

Molecular and Biochemical Parasitology

Volumn 122, Issue 1, 2002, Pages 91-94

  Hussein, Ayman S ^a   Kichenin, Ketty ^a   Selkirk, Murray E ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Acetylcholinesterase; Nematode; Nippostrongylus; RNA interference

Indexed keywords

ACETYLCHOLINESTERASE; COMPLEMENTARY DNA; RNA;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME RELEASE; EXPRESSED SEQUENCE TAG; NIPPOSTRONGYLUS BRASILIENSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ACETYLCHOLINESTERASE; ANIMALS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE SILENCING; GENES, HELMINTH; NIPPOSTRONGYLUS; RATS; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; RNA, UNTRANSLATED; SENSITIVITY AND SPECIFICITY; STRONGYLIDA INFECTIONS; TIME FACTORS;

ANIMALIA; NIPPOSTRONGYLUS; NIPPOSTRONGYLUS BRASILIENSIS;

EID: 0036079595     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(02)00068-3     Document Type: Article

References (18)

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3. Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed
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5. Specific interference by ingested dsRNA
6. RNAi in C. elegans: Soaking in the genome sequence
7. Cloning, expression, and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis
8. Determinants of substrate specificity of a second non-neuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis
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10. Purification and properties of monomeric (G1) forms of acetylcholinesterase secreted by Nippostrongylus brasiliensis
11. A new and rapid colorimetric determination of acetylcholinesterase activity
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14. RNA interference is mediated by 21 and 22 nt RNAs
15. Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes
16. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans
17. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
18. A 'direct-coloring' method for cholinesterases