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Volumn 14, Issue 2, 2003, Pages 177-193

Deciphering plant-pathogen communication: Fresh perspectives for molecular resistance breeding

Author keywords

[No Author keywords available]

Indexed keywords

VEGETABLE PROTEIN;

EID: 0038404717     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(03)00035-1     Document Type: Review
Times cited : (490)

References (80)
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    • Nuclear localization of NPR1 is required for activation of PR gene expression
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    • •].
    • •].
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    • Direct visualization of protein interactions in plant cells
    • •], a dominant-negative effect was created and thereby revealed that TGA2 and NPR1 interact in planta. Additional genetic data demonstrated that TGA2 is SA responsive. The studies described also confirmed in vivo the NPR1-TGA2 interaction, using a fluorescent technology called the protein fragment complementation assay (Table 5).
    • •], a dominant-negative effect was created and thereby revealed that TGA2 and NPR1 interact in planta. Additional genetic data demonstrated that TGA2 is SA responsive. The studies described also confirmed in vivo the NPR1-TGA2 interaction, using a fluorescent technology called the protein fragment complementation assay (Table 5).
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    • The dir1-1 mutant was isolated by transfer-DNA insertion. The dir1-1 plants exhibit wild type local responses to avirulent and virulent P. syringae, but failed to develop the SAR response leading to PR protein accumulation in the distal non-inoculated leaves. Petiole exudate experiments revealed that dir1-1 mutants were defective in an essential mobile signal emitted from the initially infected leaf. DIR1 encodes a putative apoplastic lipid-transfer-like protein (LTP) and contains the eight conserved cysteine residues found in all LTPs. The authors propose that SAR is mediated by the DIR1 protein interacting with a lipid-derived molecule to promote long-distance signalling. DIR1 was found not to be required for local or systemically induced accumulation of free SA.
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    • McDonald B.A., Linde C. Pathogen population genetics, evolutionary potential, and durable resistance. Annu. Rev. Phytopathol. 40:2002;349-379 This article proposes a framework for predicting the evolutionary potential of pathogen populations based on an analysis of their genetic structure. A matrix containing nine distinct scenarios is presented. Pathogens predicted as the most likely to overcome R-gene-mediated resistance are those capable of sexual and asexual reproduction, those with a high potential for genotype flow, with large effective population sizes and a high mutation rate. Many examples of high, intermediate and low-risk pathogens are given. The authors propose general guidelines for a rational approach to breed durable resistance based on the pathogen's evolutionary potential.
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    • Arabidopsis NPR1 was overexpressed in rice under the control of either a ubiquitin or 35S promoter. The transgenic NPR1 rice plants displayed enhanced resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae. This is the first result to suggest that monocotyledonous and dicotyledonous plants share a conserved signalling pathway involving NPR1.
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