Domain swapping and gene shuffling identify sequences required for induction of an Avr-dependent hypersensitive response by the tomato Cf-4 and Cf-9 proteins

Plant Cell

Volumn 13, Issue 2, 2001, Pages 255-272

  Wulff, B B H ^a   Thomas, C M ^a   Smoker, M ^a   Grant, M ^a   Jones, J D J ^a  

^a NORWICH RESEARCH PARK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CLADOSPORIUM; CLADOSPORIUM FULVUM; EMBRYOPHYTA; FUNGI; LYCOPERSICON ESCULENTUM; NICOTIANA TABACUM;

EID: 0035096288     PISSN: 10404651     EISSN: None     Source Type: Journal    
DOI: 10.1105/tpc.13.2.255     Document Type: Article

References (32)

1. DNA shuffling of a family of genes from diverse species accelerates directed evolution
2. The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number
3. Genetic complexity of pathogen perception by plants: The example of Rcr3, a tomato gene required specifically by Cf-2
4. Structure, function and evolution of plant disease resistance genes
5. Stable transfer of intact-high-molecular-weight DNA into plant chromosomes
6. Developmentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease-resistance gene Cf-9
7. Functional expression of a fungal avirulence gene from a modified Potato Virus X
8. The tomato Cf-9 disease resistance gene functions in tomato and potato to confer responsiveness to the fungal avirulence gene product Avr9
9. The role of leucine-rich repeat proteins in plant defences
10. Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging
11. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants
12. Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulence gene
13. The biotrophic fungus Cladosporium fulvum circumvents Cf-4-mediated resistance by producing unstable AVR4 elicitors
14. Structural diversity of leucine-rich repeat proteins
15. The fungal gene Avr9 and the oomycete gene inf1 confer avirulence to potato virus X on tobacco
16. Novel family shuffling methods for the in vitro evolution of enzymes
17. The leucine-rich repeat: A versatile binding motif
18. The specificity of polygalacturonase-inhibiting protein (PGIP): A single amino acid substitution in the solvent-exposed β-strand/β-turn region of the leucine-rich repeats (LRRs) confers a new recognition capability
19. Disruption of the avirulence gene Avr9 in 2 races of the tomato pathogen Cladosporium fulvum causes virulence on tomato genotypes with the complementary resistance gene Cf-9
20. Recombination between diverged clusters of the tomato Cf-9 plant disease resistance gene family
21. Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf4/9 locus
22. Homologues of the Cf-9 disease resistance gene (Hcr9s) are present at multiple loci on the short arm of tomato chromosome 1
23. Functional, c-myc-tagged Cf-9 resistance gene products are plasma-membrane localized and glycosylated
24. Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato
25. Rapid evolution of a protein in vitro by DNA shuffling
26. Initiation of plant disease resistance by physical interaction of AvrPto and Pto kinase
27. Characterization of the tomato Cf-4 gene for resistance to Cladosporium fulvum identifies sequences that determine recognitional specificity in Cf-4 and Cf-9
28. Genetic and molecular analysis of tomato Cf genes for resistance to Cladosporium fulvum
29. Comparison of the hypersensitive response induced by the tomato Cf-4 and Cf-9 genes in Nicotiana spp.
30. Molecular analysis of the avirulence gene Avr9 of the fungal pathogen Cladosporium fulvum fully supports the gene-for-gene hypothesis
31. Agroinfiltration is a versatile tool that facilitates comparative analyses of Avr9/Cf-9-induced and Avr4/Cf-4-induced necrosis
32. Optimization of DNA shuffling for high fidelity recombination