Metabolic pathway engineering in lactic acid bacteria

Current Opinion in Biotechnology

Volumn 14, Issue 2, 2003, Pages 232-237

  Kleerebezem, Michiel ^a   Hugenholtz, Jeroen ^a  

^a NIZO FOOD RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

POLYSACCHARIDE; PYRUVIC ACID DERIVATIVE; VITAMIN B GROUP;

BIOSYNTHESIS; BIOTECHNOLOGICAL PROCEDURES; CARBON METABOLISM; CONSUMER; ENERGY METABOLISM; FOOD MICROBIOTECHNOLOGY; GENE SEQUENCE; GENOME ANALYSIS; HUMAN; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS; METABOLIC ENGINEERING; METABOLITE; MICROBIAL METABOLISM; NONHUMAN; PRIORITY JOURNAL; REVIEW;

BACTERIA (MICROORGANISMS); LACTOCOCCUS LACTIS; NEGIBACTERIA; STREPTOCOCCUS;

EID: 0038066313     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(03)00033-8     Document Type: Review

References (35)

1. Kuipers O.P., de Ruyter P.G.G.A., Kleerebezem M., de Vos W.M. Quorum sensing-controlled gene expression systems in lactic acid bacteria. J. Biotechnol. 64:1998;15-21.
2. de Vos W.M. Gene expression systems for lactic acid bacteria. Curr. Opin. Microbiol. 2:1999;289-295.
3. Jensen P.R., Hammer K. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol. 64:1998;82-87.
4. Jensen P.R., Hammer K. Artificial promoters for metabolic optimization. Biotechnol. Bioeng. 58:1998;191-195.
5. Solem C., Jensen P.R. Modulation of gene expression made easy. Appl. Environ. Microbiol. 68:2002;2397-2403 This paper describes a very elegant and efficient system to introduce synthetic promoters upstream of a chosen gene in the chromosome of Lactococcus lactis. The system allows modulation of gene expression at a chosen level.
6. Bron P.A., Benchimol M.G., Lambert J., Palumbo E., Deghorain M., Delcour J., De Vos W.M., Kleerebezem M., Hols P. Use of the alr gene as a food-grade selection marker in lactic acid bacteria. Appl. Environ. Microbiol. 68:2002;5663-5670.
7. +-ATPase complex is essential for growth of Lactococcus lactis. J. Bacteriol. 182:2000;4738-4743.
8. Kleerebezem M., Beerthuyzen M.M., Vaughan E.E., de Vos W.M., Kuipers O.P. Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp. Appl. Environ. Microbiol. 63:1997;4581-4584.
9. Pavan S., Hols P., Delcour J., Geoffroy M.C., Grangette C., Kleerebezem M., Mercenier A. Adaptation of the nisin-controlled expression system in Lactobacillus plantarum: a tool to study in vivo biological effects. Appl. Environ. Microbiol. 66:2000;4427-4432.
10. Eichenbaum Z., Federle M.J., Marra D., de Vos W.M., Kuipers O.P., Kleerebezem M., Scott J.R. Use of the lactococcal nisA promoter to regulate gene expression in Gram-positive bacteria: comparison of induction level and promoter strength. Appl. Environ. Microbiol. 64:1998;2763-2769.
11. Bryan E.M., Bae T., Kleerebezem M., Dunny G.M. Improved vectors for nisin-controlled expression in Gram-positive bacteria. Plasmid. 44:2000;183-190.
12. Hugenholtz J., Kleerebezem M., Starrenburg M., Delcour J., de Vos W., Hols P. Lactococcus lactis as a cell factory for high-level diacetyl production. Appl. Environ. Microbiol. 66:2000;4112-4114.
13. Hols P., Kleerebezem M., Schanck A.N., Ferain T., Hugenholtz J., Delcour J., de Vos W.M. Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering. Nat. Biotechnol. 17:1999;588-592.
14. De Vuyst L., Degeest B. Heteropolysaccharides from lactic acid bacteria. FEMS Microbiol. Rev. 23:1999;153-177.
15. van Kranenburg R., Kleerebezem M., de Vos W.M. Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000. Plasmid. 43:2000;130-136.
16. van Kranenburg R., Marugg J.D., van Swam I., Willem N.J., de Vos W.M. Molecular characterization of the plasmid-encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactis. Mol. Microbiol. 24:1997;387-397.
17. van Kranenburg R., Boels I.C., Kleerebezem M., de Vos W.M. Genetics and engineering of microbial exopolysaccharides for food: approaches for the production of existing and novel polysaccharides. Curr. Opin. Biotechnol. 10:1999;498-504.
18. Kleerebezem M., Boels I.C., Groot M.N., Mierau I., Sybesma W., Hugenholtz J. Metabolic engineering of Lactococcus lactis: the impact of genomics and metabolic modelling. J. Biotechnol. 98:2002;199-213.
19. van Kranenburg R., Vos H.R., van Swam I., Kleerebezem M., de Vos W.M. Functional analysis of glycosyltransferase genes from Lactococcus lactis and other Gram-positive cocci: complementation, expression, and diversity. J. Bacteriol. 181:1999;6347-6353.
20. van Kranenburg R., van Swam I., Marugg J.D., Kleerebezem M., de Vos W.M. Exopolysaccharide biosynthesis in Lactococcus lactis NIZO B40: functional analysis of the glycosyltransferase genes involved in synthesis of the polysaccharide backbone. J. Bacteriol. 181:1999;338-340.
21. Stingele F., Vincent S.J., Faber E.J., Newell J.W., Kamerling J.P., Neeser J.R. Introduction of the exopolysaccharide gene cluster from Streptococcus thermophilus Sfi6 into Lactococcus lactis MG1363: production and characterization of an altered polysaccharide. Mol. Microbiol. 32:1999;1287-1295.
22. Gilbert C., Robinson K., Le Page R.W., Wells J.M. Heterologous expression of an immunogenic pneumococcal type 3 capsular polysaccharide in Lactococcus lactis. Infect. Immun. 68:2000;3251-3260.
23. 31P NMR. J. Biotechnol. 77:2000;17-23.
24. Looijesteijn P.J., Boels I.C., Kleerebezem M., Hugenholtz J. Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source. Appl. Environ. Microbiol. 65:1999;5003-5008.
25. + and NADH pools determined in vivo by 13C NMR. J. Biol. Chem. 277:2002;28088-28098.
26. 1-ATPase results in uncoupling of glycolysis from biomass production in Lactococcus lactis. Appl. Environ. Microbiol. 68:2002;4274-4282.
27. Koebmann B.J., Andersen H.W., Solem C., Jensen P.R. Experimental determination of control of glycolysis in Lactococcus lactis. Antonie Van Leeuwenhoek. 82:2002;237-248.
28. Boels I.C., Kleerebezem M., de Vos W.M. Engineering of carbon distribution between glycolysis and sugar nucleotide biosynthesis in Lactococcus lactis. Appl. Environ. Microbiol. 69:2003;1129-1135.
29. Grossiord B.P., Luesink E.J., Vaughan E.E., Arnaud A., De Vos W.M. Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway. J. Bacteriol. 185:2003;870-878.
30. Boels I.C., Ramos A., Kleerebezem M., de Vos W.M. Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis. Appl. Environ. Microbiol. 67:2001;3033-3040.
31. Hugenholtz J., Sybesma W., Groot M.N., Wisselink W., Ladero V., Burgess K., van Sinderen D., Piard J.C., Eggink G., Smid E.J.et al. Metabolic engineering of lactic acid bacteria for the production of nutraceuticals. Antonie Van Leeuwenhoek. 82:2002;217-235.
32. Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome. Res. 11:2001;731-753.
33. Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.K., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.et al. Complete genome sequence of Lactobacillus plantarum WCFS1. Proc. Natl. Acad. Sci. U.S.A. 100:2003;1990-1995.
34. Klaenhammer T., Altermann E., Arigoni F., Bolotin A., Breidt F., Broadbent J., Cano R., Chaillou S., Deutscher J., Gasson M.et al. Discovering lactic acid bacteria by genomics. Antonie Van Leeuwenhoek. 82:2002;29-58 The authors provide an overview of LAB genome sequencing projects that are ongoing world-wide. Several aspects of importance for LAB functionality are described in a comparative manner and highlight diversity within this group of bacteria.
35. Hoefnagel M.H., Starrenburg M.J., Martens D.E., Hugenholtz J., Kleerebezem M., Van S. II, Bongers R., Westerhoff H.V., Snoep J.L. Metabolic engineering of lactic acid bacteria, the combined approach: kinetic modelling, metabolic control and experimental analysis. Microbiology. 148:2002;1003-1013 This paper describes the construction of a kinetic model of lactococcal pyruvate metabolism. The model is based on the characteristics of the enzymes involved and can be used to evaluate the metabolic effects of modulations of individual gene expression levels. This model is made available on the web ( http://jjj.biochem.sun.ac.za ).