Systematic cloning of Treponema pallidum open reading frames for protein expression and antigen discovery

Genome Research

Volumn 13, Issue 7, 2003, Pages 1665-1674

  McKevitt, Matthew ^a   Patel, Krupa ^a   Smajs, David ^b   Marsh, Michael ^a   McLoughlin, Melanie ^b   Norris, Steven J ^b   Weinstock, George M ^a   Palzkill, Timothy ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BACTERIAL ANTIGEN; DNA TOPOISOMERASE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; SIGNAL PEPTIDE;

ANTIGENICITY; ARTICLE; BACTERIAL GENOME; CONTROLLED STUDY; DNA VECTOR; ESCHERICHIA COLI; GENE SEQUENCE; GENETIC CODE; MALE; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; RABBIT; SEQUENCE TAGGED SITE; SYPHILIS; TREPONEMA PALLIDUM;

NON-PROGRAMMATIC;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; CLONING, MOLECULAR; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; OPEN READING FRAMES; RECOMBINANT PROTEINS; TREPONEMA PALLIDUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; ORYCTOLAGUS CUNICULUS; PROKARYOTA; SPIROCHAETALES; TREPONEMA; TREPONEMA PALLIDUM; TREPONEMA PALLIDUM SUBSP. PALLIDUM;

EID: 0037810488     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.288103     Document Type: Article

References (31)

1. Blanco, D.R., Champion, C.I., Miller, J.N., and Lovett, M.A. 1988. Antigenic and structural characterization of Treponema pallidum (Nichols strain) endoflagella. Infect. Immun. 56: 168-175.
2. Blanco, D.R., Reimann, K., Skare, J., Champion, C.I., Foley, D., Exner, M.M., Hancock, R.E., Miller, J.N., and Lovett, M.A. 1994. Isolation of the outer membranes from Treponema pallidum and Treponema vincentii. J. Bacteriol. 176: 6088-6099.
3. Blanco, D.R., Miller, J.N., and Lovett, M.A. 1997. Surface antigens of the syphilis spirochete and their potential as virulence determinants. Emerg. Infect. Dis. 3: 11-20.
4. Blanco, D.R., Champion, C.I., Lewinski, M.A., Shang, E.S., Simkins, S.G., Miller, J.N., and Lovett, M.A. 1999. Immunization with Treponema pallidum outer membrane vesicles induces high-titer complement-dependent treponemicidal activity and aggregation of T. pallidum rare outer membrane proteins (TROMPs). J. Immunol. 163: 2741-2746.
5. Borenstein, L.A., Radolf, J.D., Fehniger, T.E., Blanco, D.R., Miller, J.N., and Lovett, M.A. 1988. Immunization of rabbits with recombinant Treponema pallidum surface antigen 4D alters the course of experimental syphilis. J. Immunol. 140: 2415-2421.
6. Cameron, C.E., Castro, C., Lukehart, S.A., and Van Voorhis, W.C. 1998. Function and protective capacity of Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase. Infect. Immun. 66: 5763-5770.
7. Cameron, C.E., Lukehart, S.A., Castro, C., Molini, B., Godornes, C., and Van Voorhis, W.C. 2000. Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp92. J. Infect. Dis. 181: 1401-1413.
8. Centurion-Lara, A., Arroll, T., Castillo, R., Shaffer, J.M., Castro, C., Van Voorhis, W.C., and Lukehart, S.A. 1997. Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: Genetic and antigenic analyses. Infect. Immun. 65: 1440-1444.
9. Centurion-Lara, A., Castro, C., Barrett, L., Cameron, C., Mostowfi, M., Van Voorhis, W.C., and Lukehart, S.A. 1999. Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response. J. Exp. Med. 189: 647-656.
10. Champion, C.I., Miller, J.N., Borenstein, L.A., Lovett, M.A., and Blanco, D.R. 1990. Immunization with Treponema pallidum endoflagella alters the course of experimental rabbit syphilis. Infect. Immun. 58: 3158-3161.
11. Fraser, C.M., Norris, S.J., Weinstock, G.M., White, O., Sutton, G.G., Dodson, R., Gwinn, M., Hickey, E.K., Clayton, R., Ketchum, K.A., et al. 1998. Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 281: 375-388.
12. Hardham, J.M., Stamm, L.V., Porcella, S.F., Frye, J.G., Barnes, N.Y., Howell, J.K., Mueller, S.L., Radolf, J.D., Weinstock, G.M., and Norris, S.J. 1997. Identification and transcriptional analysis of a Treponema pallidum operon encoding a putative ABC transport system, an iron-activated repressor protein homolog, and a glycolytic pathway enzyme homolog. Gene 197: 47-64.
13. Liu, Q., Li, M.Z., Leibham, D., Cortez, D., and Elledge, S.J. 1998. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 8: 1300-1309.
14. Lukehart, S.A., Baker-Zander, S.A., and Sell, S. 1986. Characterization of the humoral immune response of the rabbit to antigens of Treponema pallidum after experimental infection and therapy. Sex. Transm. Dis. 13: 9-15.
15. Miller, J.N. 1973. Immunity in experimental syphilis, VI: Successful vaccination of rabbits with Treponema pallidum, Nichols strain, attenuated by irradiation. J. Immunol. 110: 1206-1215.
16. Norris, S.J. 1993. Polypeptides of Treponema pallidum: Progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group. Microbiol. Rev. 57: 750-779.
17. Norris, S.J., Cox, D.L., and Weinstock, G.M. 2001. Biology of Treponema pallidum: Correlation of functional activities with genome sequence data. J. Mol. Microbiol. Biotechnol. 3: 37-62.
18. Radolf, J.D. 1995. Treponema pallidum and the quest for outer membrane proteins. Mol. Microbiol. 16: 1067-1073.
19. Radolf, J.D., Chamberlain, N.R., Clausell, A., and Norgard, M.V. 1988. Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114. Infect. Immun. 56: 490-498.
20. Radolf, J.D., Robinson, E.J., Bourell, K.W., Akins, D.R., Porcella, S.F., Weigel, L.M., Jones, J.D., and Norgard, M.V. 1995. Characterization of outer membranes isolated from Treponema pallidum, the syphilis spirochete. Infect. Immun. 63: 4244-4252.
21. Sambrook, J., Maniatis, T., and Fritsch, E.F. 1989. Molecular cloning : A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Sehr, P., Zumbach, K., and Pawlita, M. 2001. A generic capture ELISA for recombinant proteins fused to glutathione S-transferase: Validation for HPV serology. J. Immunol. Meth. 253: 153-162.
23. Shevchenko, D.V., Akins, D.R., Robinson, E.J., Li, M., Shevchenko, O.V., and Radolf, J.D. 1997. Identification of homologs for thioredoxin, peptidyl prolyl cis-trans isomerase, and glycerophosphodiester phosphodiesterase in outer membrane fractions from Treponema pallidum, the syphilis spirochete. Infect. Immun. 65: 4179-4189.
24. Shevchenko, D.V., Sellati, T.J., Cox, D.L., Shevchenko, O.V., Robinson, E.J., and Radolf, J.D. 1999. Membrane topology and cellular location of the Treponema pallidum glycerophosphodiester phosphodiesterase (GlpQ) ortholog. Infect. Immun. 67: 2266-2276.
25. Shuman, S. 1994. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase. J. Biol. Chem. 269: 32678-32684.
26. Smajs, D., McKevitt, M., Wang, L., Howell, J.K., Norris, S.J., Palzkill, T., and Weinstock, G.M. 2002. BAC library of T. pallidum DNA in E. coli. Genome Res. 12: 515-522.
27. Stamm, L.V., Hardham, J.M., and Frye, J.G. 1996. Expression and sequence analysis of a Treponema pallidum gene, tpn38(b), encoding an exported protein with homology to T. pallidum and Borrelia burgdorferi proteins. FEMS Microbiol. Lett. 135: 57-63.
28. Sternberg, N., Hamilton, D., Austin, S., Yarmolinsky, M., and Hoess, R. 1981. Site-specific recombination and its role in the life cycle of bacteriophage P1. Cold Spring Harb. Symp. Quant. Biol. 45: 297-309.
29. Strugnell, R., Cockayne, A., and Penn, C.W. 1990. Molecular and antigenic analysis of treponemes. Crit. Rev. Microbiol. 17: 231-250.
30. Weinstock, G.M., Hardham, J.M., McLeod, M.P., Sodergren, E.J., and Norris, S.J. 1998. The genome of Treponema pallidum: New light on the agent of syphilis. FEMS Microbiol. Rev. 22: 323-332.
31. Wicher, K., Schouls, L.M., Wicher, V., Van Embden, J.D., and Nakeeb, S.S. 1991. Immunization of guinea pigs with recombinant TmpB antigen induces protection against challenge infection with Treponema pallidum Nichols. Infect. Immun. 59: 4343-4348.