A generic capture ELISA for recombinant proteins fused to glutathione S-transferase: Validation for HPV serology

Journal of Immunological Methods

Volumn 253, Issue 1-2, 2001, Pages 153-162

  Sehr, Peter ^a   Zumbach, Klaus ^a   Pawlita, Michael ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Antigen capture; ELISA; GST fusion protein; HPV; Immobilized glutathione

Indexed keywords

ANTIGEN; CASEIN; EPITOPE; GLUTATHIONE TRANSFERASE; MONOCLONAL ANTIBODY; PROTEIN E6; PROTEIN E7; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ANTIGEN EXPRESSION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LYSATE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PURIFICATION; SEROLOGY; UTERINE CERVIX CARCINOMA; VALIDATION PROCESS; WART VIRUS;

ANTIBODIES, VIRAL; ANTIGENS, VIRAL, TUMOR; CARCINOMA; CASEINS; DNA-BINDING PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HUMANS; ONCOGENE PROTEINS, VIRAL; PAPILLOMAVIRIDAE; PAPILLOMAVIRUS INFECTIONS; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SENSITIVITY AND SPECIFICITY; TUMOR VIRUS INFECTIONS; UTERINE CERVICAL NEOPLASMS;

HUMAN PAPILLOMAVIRUS; HUMAN PAPILLOMAVIRUS TYPES;

EID: 0035400487     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00376-3     Document Type: Article

References (18)

1. A general purification protocol for E7 proteins from "high- and low-risk" human papillomavirus types expressed in the yeast Schizosaccharomyces pombe
2. Coefficient of agreement for nominal scales
3. Anomalous behavior of bovine alpha s1- and beta-caseins on gel electrophoresis in sodium dodecyl sulfate buffers
4. Adsorption of the protein antigen myoglobin affects the binding of conformation-specific monoclonal antibodies
5. Antibodies against linear and conformational epitopes of human papillomavirus type 16 that independently associate with incident cervical cancer
6. Papillomaviruses and human oncogenesis
7. Monoclonal antibodies specific for the carboxy terminus of simian virus 40 large T antigen
8. Antibodies against early proteins of human papillomaviruses as diagnostic markers for invasive cervical cancer
9. Antibodies to HPV-16 E6 and E7 proteins as markers for HPV-16-associated invasive cervical cancer
10. Production of glutathione-coated microtitre plates for capturing recombinant glutathione S-transferase fusion proteins as antigens in immunoassays
11. Antibodies against linear and conformational epitopes of the human papillomavirus (HPV) type 16 E6 and E7 oncoproteins in sera of cervical cancer patients
12. An ELISA sandwich capture assay for recombinant fusion proteins containing glutathione-S-transferase
13. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
14. Human papillomavirus (HPV) infection, HIV infection and cervical cancer in Tanzania, east Africa
15. Serologic response in human papillomavirus-associated invasive cervical cancer
16. Structure and function of glutathione S-transferases
17. Viruses in human cancers