Immunization with Treponema pallidum outer membrane vesicles induces high-titer complement-dependent treponemicidal activity and aggregation of T. pallidum rare outer membrane proteins (TROMPs)

Journal of Immunology

Volumn 163, Issue 5, 1999, Pages 2741-2746

  Blanco, David R ^a   Champion, Cheryl I ^a   Lewinski, Michael A ^b   Shang, Ellen S ^a   Simkins, Stephen G ^c   Miller, James N ^a   Lovett, Michael A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b QUEST DIAGNOSTICS NICHOLS INSTITUTE   (United States)

^c NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BACTERIAL VACCINE; OUTER MEMBRANE PROTEIN; TREPONEMA PALLIDUM VACCINE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANTIBACTERIAL ACTIVITY; ANTIBODY RESPONSE; ANTIBODY TITER; ARTICLE; COMPLEMENT DEPENDENT CYTOTOXICITY; FLUORESCENT TREPONEMA ANTIBODY TEST; FREEZE FRACTURE; IMMUNIZATION; IMMUNOREACTIVITY; MEMBRANE VESICLE; MOUSE; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN AGGREGATION; SUBCUTANEOUS DRUG ADMINISTRATION; SYPHILIS; SYPHILIS SEROLOGY; TREPONEMA PALLIDUM;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BLOOD BACTERICIDAL ACTIVITY; CELL MEMBRANE; COMPLEMENT SYSTEM PROTEINS; FEMALE; IMMUNE SERA; LIPOPROTEINS; MICE; MICE, INBRED BALB C; PORINS; RABBITS; SYPHILIS; TREPONEMA IMMOBILIZATION TEST; TREPONEMA PALLIDUM;

EID: 0033198208     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Cox, D. L, B. Riley, P. Chang, S. Sayahtaheri, S. Tassell, and J. Hevelone. 1990. Effects of molecular oxygen, oxidation-reduction potential, and antioxidants upon in vitro replication of Treponema pallidum subsp. pallidum. Appl. Environ. Microbiol. 56:3063.
2. Norris, S. J., and D. G. Edmondson. 1986. Factors affecting the multiplication and subculture of Treponema pallidum subsp. pallidum in a tissue culture system. Infect. Immun. 53:534.
3. Radolf, J. D., M. V. Norgard, and W. W. Shulz. 1989. Outer membrane ultrastructure explains the limited antigenicity of virulent Treponema pallidum. Proc. Natl. Acad. Sci. USA 86:2051.
4. Walker, E. M., G. A. Zamphigi, D. R. Blanco, J. N. Miller, and M. A. Lovett. 1989. Demonstration of rare protein in the outer membrane of Treponema pallidum subsp. pallidum by freeze-fracture analysis. J. Bacteriol. 171:5005.
5. Blanco, D. R., E. M. Walker, D. A. Haake, C. I. Champion, J. N. Miller, and M. A. Lovett. 1990. Complement activation limits the rate of in vitro treponemicidal activity and correlates with antibody-mediated aggregation of Treponema pallidum rare outer membrane protein (TROMP). J. Immunol. 144:1914.
6. Blanco, D. R., K. Reimann, J. Skare, C. I. Champion, D. Foley, M. M. Exner, R. E. W. Hancock, J. N. Miller, and M. A. Lovett. 1994. Isolation of the outer membranes from Treponema pallidum and Treponema vincentii. J. Bacteriol. 176:6088.
7. Akins, D. R., B. K. Purcell, M. M. Mitra, M. V. Norgard, and J. D. Radolf. 1993. Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphilicity. Infect. Immun. 61:1202.
8. Schouls, L. M., R. Mout, J. Dekker, and J. D. A. van Embden. 1989. Characterization of lipid-modified immunogenic proteins of Treponema pallidum expressed in Escherichia coli. Microb. Pathog. 7:175.
9. Radolf, J. D. 1995. Treponema pallidum and the quest for outer membrane proteins. Mol. Microbiol. 6:1067.
10. Blanco, D. R., C. I. Champion, M. M. Exner, H. Erdjument-Bromage, R. E. W. Hancock, P. Tempst, J. N. Miller, and M. A. Lovett. 1995. Porin activity and sequence analysis of a 31-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Trompl). J. Bacteriol. 177:3556.
11. Champion, C. I., D. R. Blanco, H. Erdjument-Bromage, M. M. Exner, R. E. W. Hancock, P. Tempst, J. N. Miller, and M. A. Lovett. 1997. Sequence analysis and recombinant expression of a 28-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Tromp2). J. Bacteriol. 179:1230.
12. Blanco, D. R., C. I. Champion, M. M. Exner, E. S. Shang, J. T. Skare, R. E. W. Hancock, J. N. Miller, and M. A. Lovett. 1996. Recombinant Treponema pallidum rare outer membrane protein 1 (Tromp1) expressed in Escherichia coli has porin activity and surface antigenic exposure. J. Bacteriol. 178:6685.
13. Bishop, N. H., and J. N. Miller. 1983. Humoral immune mechanisms in acquired syphilis. In Pathogenesis and Immunology of Treponemal Infection. R. F. Schell and D. M. Musher, eds. Marcel Dekker, New York, p. 241.
14. Bishop, N. H., and J. N. Miller. 1976. Humoral immunity in experimental syphilis: the relationship of neutralizing factors in immune serum to acquired resistance. J. Immunol. 117:197.
15. Azadegan, A. A., R. F. Schell, and J. L. LeFrock. 1983. Immune serum confers protection against syphilitic infection on hamsters. Infect. Immun. 42:42.
16. Bishop, N. H., and J. N. Miller. 1976. Humoral immunity in experimental syphilis: the demonstration of resistance conferred by passive immunization. J. Immunol. 117:191.
17. Pavia, C. S., C. J. Niederbuhl, and J. Saunders. 1985. Antibody-mediated protection of guinea-pigs against infection with Treponema pallidum. Immunology 56:195.
18. Perine, P. L., R. S. Weiser, and S. J. Klebanoff. 1973. Immunity to syphilis: passive transfer in rabbits with hyperimmune serum. Infect. Immun. 8:787.
19. Schell, R. F., J. K. Chan, and J. L. LeFrock. 1979. Endemic syphilis: passive transfer of resistance with serum and cells in hamsters. J. Infect. Dis. 140:378.
20. Sepetijian, M., D. Salussola, and J. Thivolet. 1973. Attempt to protect rabbits against experimental syphilis by passive immunization. Br. J. Vener. Dis. 49:335.
21. Weiser, R. S., D. Erickson, P. S. Perine, and N. N. Pearsall. 1976. Immunity to syphilis: passive transfer in rabbits using serial doses of immune serum. Infect. Immun. 13:1402.
22. Wicher, K., J. Zabek, and V. Wicher. 1992. Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs. Infect. Immun. 60:3217.
23. Towbin, H., T. Staechelin, and J. Gordon. 1979. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350.
24. 1964 and 1969. Manual of Serologic Tests for Syphilis. U.S. Department of Health, Education, and Welfare, Public Health Service, National Centers for Disease Control, Atlanta, GA.
25. Nelson, R. A., Jr., and M. M. Mayer. 1949. Immobilization of Treponema pallidum in vitro by antibody produced in syphilitic infection. J. Exp. Med. 89:369.
26. Norris, S. J. 1993. Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. The Treponema pallidum polypeptide research group. Microbiol. Rev. 57:750.
27. Purcell, B. K., N. R. Chamberlain, M. S. Goldberg, L. P. Andrews, E. J. Robinson, M. V. Norgard, and J. D. Radolf. 1989. Molecular cloning and characterization of the 15-kilodalton major immunogen of Treponema pallidum. Infect. Immun. 57:3708.
28. Purcell, B. K., M. A. Swancutt, and J. D. Radolf. 1990. Lipid modification of the 15-kilodalton major membrane immunogen of Treponema pallidum. Mol. Microbiol. 4:1371.
29. Swancutt, M. A., B. S. Riley, J. D. Radolf, and M. V. Norgard. 1989. Molecular characterization of the pathogen-specific, 34-kilodalton membrane immunogen of Treponema pallidum. Infect. Immun. 57:3314.
30. Radolf, J. D., E. J. Robinson, K. W. Bourell, D. R. Akins, S. F. Porcella, L. M. Weigel, J. D. Jones, and M. V. Norgard. 1995. Characterization of outer membranes isolated from Treponema pallidum, the syphilis spirochete. Infect. Immun. 63:4244.
31. Rosahn, P. D., B. Gueft, and C. L. Rowe. 1948. Experimental mouse syphilis: organ distribution of the infectious agent. Am. J. Syph. 32:327.
32. Rosahn, P. D., and C. L. Rowe. 1950. Experimental mouse syphilis: minimal infectious number of Treponema pallidum. Am. J. Syph. 34:40.
33. Marchitto, K. S., C. K. Selland-Grossling, and M. V. Norgard. 1986. Molecular specificities of monoclonal antibodies directed against virulent Treponema pallidum. Infect. Immun. 51:168.
34. Norgard, M. V., N. R. Chamberlain, M. A. Swancutt, and M. S. Goldberg. 1986. Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli. Infect. Immun. 54:500.
35. Chamberlain, N. R., M. E. Brandt, A. L. Erwin, J. D. Radolf, and M. V. Norgard. 1989. Major integral membrane protein immunogens of Treponema pallidum are proteolipids. Infect. Immun. 57:2872.
36. Coon, J., and R. Hunter. 1975. Properties of conjugated protein immunogens which selectively stimulate delayed-type hypersensitivity. J. Immunol. 114:1518.