Modulation of translation-initiation in CHO-K1 cells by rapamycin-induced heterodimerization of engineered eIF4G fusion proteins

Biotechnology and Bioengineering

Volumn 83, Issue 2, 2003, Pages 210-225

  Schlatter, Stefan ^a,b   Senn, Claudia ^a,c   Fussenegger, Martin ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Cap; eIF; eIF4G; FKBP; FRB; Internal ribosome entry (IRES); Luciferase; Multicistronic expression; Protease 2A; Rapamycin; SEAP; Tetracycline; Translation control system (TCS); Urokinase; UTR

Indexed keywords

ENZYMES; GENES; PROTEINS; SYNTHESIS (CHEMICAL);

TRANSLATION CONTROL SYSTEM (TCS);

CELLS;

ALKALINE PHOSPHATASE PLACENTA ISOENZYME; CAPPED RNA; FK 506 BINDING PROTEIN; GUANOSINE PHOSPHATE; HELICASE; HYBRID PROTEIN; INITIATION FACTOR; INITIATION FACTOR 4E; INITIATION FACTOR 4F; INITIATION FACTOR 4G; LUCIFERASE; MESSENGER RNA; PROTEINASE; PROTEINASE 2A; RAPAMYCIN; RAPAMYCIN ASSOCIATED PROTEIN; RECOMBINANT INITIATION FACTOR 4G; RECOMBINANT PROTEIN; TETRACYCLINE; UNCLASSIFIED DRUG; UROKINASE;

5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CHO CELL; CHO K1 CELL; CISTRON; DIMERIZATION; FK506 BINDING PROTEIN RAPAMYCIN BINDING DOMAIN; GENE CONTROL; GENE EXPRESSION; HETERODIMERIZATION; INTERNAL RIBOSOME ENTRY SITE; LUC GENE; MAMMAL CELL; MULTICISTRONIC EXPRESSION; NONHUMAN; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN SYNTHESIS; REPORTER GENE; RNA GENE; RNA TRANSLATION; TRANSGENE; TRANSLATION CONTROL SYSTEM; TRANSLATION INITIATION;

ANIMALS; BASE SEQUENCE; CHO CELLS; CRICETINAE; DIMERIZATION; DNA PRIMERS; EUKARYOTIC INITIATION FACTOR-4G; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIROLIMUS; TRANSFECTION;

CRICETINAE; CRICETULUS GRISEUS; EUKARYOTA; MAMMALIA; PHOTINUS PYRALIS; PYRALIS;

EID: 0037701245     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10662     Document Type: Article

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