MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways

Nature

Volumn 421, Issue 6926, 2003, Pages 957-961

  Lou, Zhenkun ^a   Minter Dykhouse, Katherine ^a   Wu, Xianglin ^a   Chen, Junjie ^a  

^a MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIODIVERSITY; GENETIC ENGINEERING; PHOSPHORUS; PROTEINS;

DAMAGE RESPONSE PATHWAYS;

DNA;

ATM PROTEIN; CHECKPOINT KINASE 2; PROTEIN; PROTEIN MDC1; PROTEIN P53; SERINE; THREONINE; UNCLASSIFIED DRUG;

DAMAGE; DNA; PROTEIN; REPAIR;

APOPTOSIS; ARTICLE; CELL CYCLE S PHASE; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; EMBRYO; GENE DELETION; HUMAN; HUMAN CELL; MAMMAL; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; TUMOR SUPPRESSOR GENE;

ANIMALS; CELL LINE; CELL NUCLEUS; DNA DAMAGE; DNA-BINDING PROTEINS; ENZYME ACTIVATION; GAMMA RAYS; HUMANS; MICE; MUTATION; NUCLEAR PROTEINS; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASES; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTEIN-SERINE-THREONINE KINASES; SIGNAL TRANSDUCTION; TRANS-ACTIVATORS; TUMOR CELLS, CULTURED;

MAMMALIA;

EID: 0037468232     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature01447     Document Type: Article

References (30)

1. Hofmann, K. & Bucher, P. The FHA domain: a putative nuclear signaling domain found in protein kinases and transcription factors. Trends Biochem. Sci. 20, 347-349 (1995).
2. Durocher, D., Henckel, J., Fersht, A. R. & Jackson, S. P. The FHA domain is a modular phosphopeptide recognition motif. Mol. Cell 4, 387-394 (1999).
3. Wang, P. et al. Structure and specificity of the interaction between the FHA2 domain of Rad53 and phosphotyrosyl peptides. J. Mol. Biol. 302, 927-940 (1999).
4. Durocher, D. et al. The molecular basis of FHA domain: phosphopeptide binding specificity and implications for phospho-dependent signaling mechanisms. Mol. Cell 6, 1169-1182 (2000).
5. Li, J., Smith, G. P. & Walker, J. C. Kinase interaction domain of kinase-associated protein phosphatase, a phosphoprotein-binding domain. Proc. Natl. Acad. Sci. USA 96, 7821-7826 (1999).
6. Zhou, B. B. & Elledge, S. J. The DNA damage response: putting checkpoints in perspective. Nature 408, 433-439 (2001).
7. Rouse, J. & Jackson, S. P. Interfaces between the detection, signaling, and repair of DNA damage. Science 297, 547-551 (2002).
8. Callebaut, I. & Mormon, J. P. From BRCA1 to RAP1: A wildspread BRCT module closely associated with DNA repair. FEBS Lett. 400, 25-30 (1997).
9. Sun, Z., Hsiao, J., Fay, D. S. & Stern, D. F. Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint. Science 281, 272-274 (1998).
10. Vialard, J. E., Gilbert, C. S., Green, C. M. & Lowndes, N. F. The budding yeast checkpoint protein rad9 is subject to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage. EMBO J. 17, 5679-5688 (1998).
11. Emiili, A. MEC1-dependent phosphorylation of Rad9p in response to DNA damage. Mol. Cell 2, 183-189 (1998).
12. Ahn, J. Y., Li, X., Davis, H. L. & Canman, C. E. Phosphorylation of threonine 68 promotes oligomerization and autophosphorylation of the CHK2 protein kinase via the forkhead-associated domain. J. Biol. Chem. 277, 19389-19395 (2002).
13. Xu, X., Tsvetkov, L. M. & Stern, D. F. Chk2 activation and phosphorylation-dependent oligomerization. Mol. Cell Biol. 22, 4419-4432 (2002).
14. Scully, R. et al. Dynamic changes of BRCA1 subnuclear localization and phosphorylation state are initiated by DNA damage. Cell 90, 425-435 (1997).
15. Ward, I. M., Wu, X. & Chen, J. Threonine 68 of Chk2 is phosphorylated at the site of DNA strand breaks. J. Biol. Chem. 276, 47755-47758 (2001).
16. Rogakou, E. P., Boon, C., Redon, C. & Bonner, W. M. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146, 905-916 (1999).
17. Paull, T. T. et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr. Biol. 10, 886-895 (2000).
18. Matsuoka, S., Huang, M. & Elledge, S. J. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282, 1893-1897 (1998).
19. Melchionna, R., Chen, X. B., Blasina, A. & McGowan, C. H. Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1. Nature Cell Biol. 2, 762-765 (2000).
20. Matsuoka, S. et al. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro. Proc. Natl. Acad. Sci. USA 97, 10389-10394 (2000).
21. Ahn, J.-H., Schwarz, J. K., Piwnica-Worms, H. & Canman, C. E. Threonine 68 phosphorylation by ataxia telangiectasia-mutated is required for efficient activation of Chk2 in response to ionizing radiation. Cancer Res. 60, 5934-5936 (2000).
22. Chaturvedi, P. et al. Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway. Oncogene 18, 4047-4054 (1999).
23. Elbashir, S. M. et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498 (2001).
24. Painter, R. B. & Young, B. R. Radiosensitivity in ataxia-telangiectasia: A new explanation. Proc. Natl. Acad. Sci. USA 77, 7315-7317 (1980).
25. Falck, J., Mailand, N., Syljuasen, R. G., Bartek, J. & Lukas, J. The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis. Nature 410, 842-847 (2001).
26. Hirao, A. et al. DNA damage-induced activation of p53 by the checkpoint kinase Chk2. Science 287, 1824-1827 (2000).
27. Hirao, A. et al. Chk2 is a tumour suppressor that regulates apoptosis in both an ataxia telangiectasia mutated (ATM)-dependent and an ATM-independent manner. Mol. Cell. Biol. 22, 6521-6532 (2002).
28. Jack, M. T. et al. Chk2 is dispensable for p53-mediated G1 arrest but is required for a latent p53-meidated apoptotic response. Proc. Natl. Acad. Sci. USA 99, 9825-9829 (2002).
29. Chehab, N. H., Malikzay, A., Apple, M. & Halazonetis, T. D. Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53. Genes Dev. 14, 278-288 (2000).
30. Shieh, S. Y., Ahn, J., Tamai, K., Taya, Y. & Pricves, C. The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites. Genes Dev. 14, 289-300 (2000).