Rat retinal ganglion cell loss caused by kainate, NMDA and ischemia correlates with a reduction in mRNA and protein of Thy-1 and neurofilament light

Brain Research

Volumn 963, Issue 1-2, 2003, Pages 298-306

  Chidlow, Glyn ^a   Osborne, Neville N ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Ischemia; Kainate; Neurofilament light; NMDA; Retinal ganglion cell; Thy 1

Indexed keywords

BIOLOGICAL MARKER; CELL MARKER; KAINIC ACID; MESSENGER RNA; N METHYL DEXTRO ASPARTIC ACID; NEUROFILAMENT PROTEIN; THY 1 ANTIGEN;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL DAMAGE; CELL DEATH; CELL LOSS; CELL SURVIVAL; COMPARATIVE STUDY; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE MARKER; IMMUNOREACTIVITY; ISCHEMIA; NEUROFILAMENT; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DETERMINATION; QUANTITATIVE ANALYSIS; RAT; REPERFUSION; RETINA; RETINA GANGLION CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; ANTIGENS, THY-1; BLOTTING, WESTERN; EXCITATORY AMINO ACID AGONISTS; EYE; IMMUNOHISTOCHEMISTRY; ISCHEMIA; KAINIC ACID; KINETICS; MALE; MICROINJECTIONS; N-METHYLASPARTATE; NEUROFILAMENT PROTEINS; RATS; RATS, WISTAR; REPERFUSION INJURY; RETINAL GANGLION CELLS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; VITREOUS BODY;

EID: 0037435931     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(02)04052-0     Document Type: Article

References (38)

1. Barnstable C.J., Drager U.C. Thy-1 antigen: a ganglion cell specific marker in rodent retina. Neuroscience. 11:1984;847-855.
2. Beale R., Osborne N.N. Localisation of the Thy-1 antigen to the surfaces of rat retinal ganglion cells. Neurochem. Int. 4:1982;587-595.
3. Brandstatter J.H., Hartveit E., Sassoe Pognetto M., Wassle H. Expression of NMDA and high-affinity kainate receptor subunit mRNAs in the adult rat retina. Eur. J. Neurosci. 6:1994;1100-1112.
4. Goto M., Inomata N., Ono H., Saito K.I., Fukuda H. Changes of electroretinogram and neurochemical aspects of GABAergic neurons of retina after intraocular injection of kainic acid in rats. Brain Res. 211:1981;305-314.
5. Hager G., Eckert E., Schwaiger F.W. Semiquantitative analysis of low levels of mRNA expression from small amounts of brain tissue by non-radioactive reverse transcriptase-polymerase chain reaction. J. Neurosci. Methods. 89:1999;141-149.
6. Harrison D.C., Medhurst A.D., Bond B.C., Campbell C.A., Davis R.P., Philpott K.L. The use of quantitative RT-PCR to measure mRNA expression in a rat model of focal ischemia - caspase-3 as a case study. Mol. Brain Res. 75:2000;143-149.
7. Hoffman P.N., Pollock S.C., Striph G.G. Altered gene expression after optic nerve transection: reduced neurofilament expression as a general response to axonal injury. Exp. Neurol. 119:1993;32-36.
8. Hoffman P.N., Cleveland D.W., Griffin J.W., Landes P.W., Cowan N.J., Price D.L. Neurofilament gene expression: a major determinant of axonal caliber. Proc. Natl. Acad. Sci. USA. 84:1987;3472-3476.
9. Kermer P., Klocker N., Weishaupt J.H., Bahr M. Transection of the optic nerve in rats: studying neuronal death and survival in vivo. Brain Res. Brain Res. Protoc. 7:2001;255-260.
10. Kong W.C., Cho E.Y. Antibodies against neurofilament subunits label retinal ganglion cells but not displaced amacrine cells of hamsters. Life Sci. 64:1999;1773-1778.
11. Lam T.T., Siew E., Chu R., Tso M.O. Ameliorative effect of MK-801 on retinal ischemia. J. Ocul. Pharmacol. Ther. 13:1997;129-137.
12. Louzada P. Jr., Dias J.J., Santos W.F., Lachat J.J., Bradford H.F., Coutinho Netto J. Glutamate release in experimental ischaemia of the retina: an approach using microdialysis. J. Neurochem. 59:1992;358-363.
13. Minzenberg M., Berkelaar M., Bray G., McKerracher L. Changes in retinal ganglion cell axons after optic nerve crush: neurofilament expression is not the sole determinant of calibre. Biochem. Cell Biol. 73:1995;599-604.
14. Nash M.S., Osborne N.N. Assessment of Thy-1 mRNA levels as an index of retinal ganglion cell damage. Invest. Ophthalmol. Vis. Sci. 40:1999;1293-1298.
15. Nash M.S., Wood J.P., Melena J., Osborne N.N. Flupirtine ameliorates ischaemic-like death of rat retinal ganglion cells by preventing calcium influx. Brain Res. 856:2000;236-239.
16. Nickells R.W. Retinal ganglion cell death in glaucoma: the how, the why, and the maybe. J. Glaucoma. 5:1996;345-356.
17. Osborne N.N., Chidlow G., Nash M.S., Wood J.P. The potential of neuroprotection in glaucoma treatment. Curr. Opin. Ophthalmol. 10:1999;82-92.
18. Osborne N.N., De Santis L.M., Bae J.H., Ugarte M., Wood J.P.M., Nash M.S., Chidlow G. Topically applied betaxolol attenuates NMDA-induced toxicity to ganglion cells and the effects of ischaemia to the retina. Exp. Eye Res. 69:1999;331-342.
19. Osborne N.N., Ugarte M., Chao M., Chidlow G., Bae J.H., Wood J.P., Nash M.S. Neuroprotection in relation to retinal ischemia and relevance to glaucoma. Surv. Ophthalmol. 43:(Suppl. 1):1999;S102-S128.
20. Otori Y., Wei J.Y., Barnstable C.J. Neurotoxic effects of low doses of glutamate on purified rat retinal ganglion cells. Invest. Ophthalmol. Vis. Sci. 39:1998;972-981.
21. Quigley H.A. Neuronal death in glaucoma. Prog. Retin. Eye Res. 18:1999;39-57.
22. Sahel J.A., Albert D.M., Lessell S., Adler H., McGee T.L., Konrad Rastegar J. Mitogenic effects of excitatory amino acids in the adult rat retina. Exp. Eye Res. 53:1991;657-664.
23. Schlamp C.L., Johnson E.C., Li Y., Morrison J.C., Nickells R.W. Changes in Thy1 gene expression associated with damaged retinal ganglion cells. Mol. Vis. 7:2001;192-201.
24. Schuettauf F., Naskar R., Vorwerk C.K., Zurakowski D., Dreyer E.B. Ganglion cell loss after optic nerve crush mediated through AMPA-kainate and NMDA receptors. Invest. Ophthalmol. Vis. Sci. 41:2000;4313-4316.
25. Schwarcz R., Coyle J.T. Kainic acid: neurotoxic effects after intraocular injection. Invest. Ophthalmol. Vis. Sci. 16:1977;141-148.
26. Selles Navarro I., Villegas Perez M.P., Salvador Silva M., Ruiz Gomez J.M., Vidal Sanz M. Retinal ganglion cell death after different transient periods of pressure-induced ischemia and survival intervals. A quantitative in vivo study. Invest. Ophthalmol. Vis. Sci. 37:1996;2002-2014.
27. Siliprandi R., Canella R., Carmignoto G., Schiavo N., Zanellato A., Zanoni R., Vantini G. N-Methyl-D-aspartate-induced neurotoxicity in the adult rat retina. Vis. Neurosci. 8:1992;567-573.
28. Sucher N.J., Aizenman E., Lipton S.A. N-Methyl-D-aspartate antagonists prevent kainate neurotoxicity in rat retinal ganglion cells in vitro. J. Neurosci. 11:1991;966-971.
29. Sucher N.J., Lipton S.A., Dreyer E.B. Molecular basis of glutamate toxicity in retinal ganglion cells. Vis. Res. 37:1997;3483-3493.
30. Suzuki T., Higgins P.J., Crawford D.R. Control selection for RNA quantitation. Biotechniques. 29:2000;332-337.
31. Vidal Sanz M., Lafuente M.P., Mayor Torroglosa S., Aguilera M.E., Miralles de Imperial J., Villegas Perez M.P. Brimonidine's neuroprotective effects against transient ischaemia-induced retinal ganglion cell death. Eur. J. Ophthalmol. 11:(Suppl. 2):2001;S36-S40.
32. Vorwerk C.K., Kreutz M.R., Dreyer E.B., Sabel B.A. Systemic L-kynurenine administration partially protects against NMDA, but not kainate-induced degeneration of retinal ganglion cells, and reduces visual discrimination deficits in adults rats. Invest. Ophthalmol. Vis. Sci. 37:1996;2382-2392.
33. Vorwerk C.K., Kreutz M.R., Bockers T.M., Brosz M., Dreyer E.B., Sabel B.A. Susceptibility of retinal ganglion cells to excitotoxicity depends on soma size and retinal eccentricity. Curr. Eye Res. 19:1999;59-65.
34. Vorwerk C.K., Lipton S.A., Zurakowski D., Hyman B.T., Sabel B.A., Dreyer E.B. Chronic low-dose glutamate is toxic to retinal ganglion cells. Toxicity blocked by memantine. Invest. Ophthalmol. Vis. Sci. 37:1996;1618-1624.
35. Vorwerk C.K., Gorla M.S.R., Dreyer E.B. An experimental basis for implicating excitotoxicity in glaucomatous optic neuropathy. Surv. Ophthalmol. 43:1999;S142-S150.
36. Wood J.P., DeSantis L., Chao H.M., Osborne N.N. Topically applied betaxolol attenuates ischaemia-induced effects to the rat retina and stimulates BDNF mRNA. Exp. Eye Res. 72:2001;79-86.
37. Yoles E., Schwartz M. Elevation of intraocular glutamate levels in rats with partial lesion of the optic nerve. Arch. Ophthalmol. 116:1998;906-910.
38. Yoles E., Muller S., Schwartz M. NMDA-receptor antagonist protects neurons from secondary degeneration after partial optic nerve crush. J. Neurotrauma. 14:1997;665-675.