Effects of methanol concentration on expression levels of recombinant protein in fed-batch cultures of Pichia methanolica

Biotechnology and Bioengineering

Volumn 81, Issue 3, 2003, Pages 291-298

  Mayson, Brian E ^a   Kilburn, Douglas G ^a   Zamost, Bruce L ^b   Raymond, Christopher K ^b   Lesnicki, Gary J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b NONE

Author keywords

Heterologous protein production; Methanol monitoring and control; Methylotrophic yeast fermentation; Pichia methanolica gene expression system

Indexed keywords

CELL CULTURE; ENZYMES; FERMENTATION; GENES; METHANOL; YEAST;

INDUCTION PHASES;

GENETIC ENGINEERING;

ALCOHOL OXIDASE; GLUCOSE; METHANOL; RECOMBINANT PROTEIN; TRANSFERRIN;

ARTICLE; BIOLOGICAL MONITORING; CONCENTRATION RESPONSE; CONTROL SYSTEM; CONTROLLED STUDY; CULTURE MEDIUM; FED BATCH CULTURE; FED BATCH FERMENTATION; FUNGUS CULTURE; FUNGUS GROWTH; GENE EXPRESSION; GENE INDUCTION; KNOCKOUT GENE; NONHUMAN; PHENOTYPE; PICHIA; PICHIA METHANOLICA; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RECOMBINANT GENE; REGULATORY MECHANISM; STRAIN DIFFERENCE; YEAST CELL;

BIOREACTORS; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; FEEDBACK; GENE EXPRESSION REGULATION, FUNGAL; METHANOL; PICHIA; PROTEIN ENGINEERING; QUALITY CONTROL; RECOMBINANT PROTEINS; SPECIES SPECIFICITY; TRANSFERRIN;

PICHIA METHANOLICA;

EID: 0037419716     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10464     Document Type: Article

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