An expression system matures: A highly efficient and cost-effective process for phytase production by recombinant strains of Hansenula polymorpha

Biotechnology and Bioengineering

Volumn 63, Issue 3, 1999, Pages 373-381

  Mayer, A F ^a   Hellmuth, K ^a   Schlieker, H ^a   Lopez Ulibarri, R ^a   Oertel, S ^a   Dahlems, U ^b   Strasser, A W M ^b   Van Loon, A P G M ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

^b Rhein Biotech GmbH   (Germany)

Author keywords

Expression system; Fermentation development; Hansenula polymorpha; High cell density cultivation; Low cost protein production; Optimizing protein secretion

Indexed keywords

CELL CULTURE; COST EFFECTIVENESS; FERMENTATION; GLUCOSE; PRODUCTION; PROTEINS; YEAST;

HANSENULA POLYMORPHA; HIGH CELL DENSITY CULTIVATION; PHYTASE PRODUCTION; PROTEIN SECRETION;

ENZYME KINETICS;

GLUCOSE; GLYCEROL; PHYTASE;

ARTICLE; COST EFFECTIVENESS ANALYSIS; ENZYME PURIFICATION; ENZYME SYNTHESIS; FERMENTATION; GENE EXPRESSION SYSTEM; HANSENULA POLYMORPHA; NONHUMAN; PROTEIN EXPRESSION;

6-PHYTASE; ASPERGILLUS; ASPERGILLUS FUMIGATUS; BIOMASS; BIOTECHNOLOGY; CLONING, MOLECULAR; COST-BENEFIT ANALYSIS; CULTURE MEDIA; FERMENTATION; GLUCOSE; GLYCEROL; KINETICS; PICHIA; PLASMIDS; RECOMBINANT PROTEINS;

PICHIA ANGUSTA;

EID: 0033526419     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990505)63:3<373::AID-BIT14>3.0.CO;2-T     Document Type: Article

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