Development of real time PCR assays for the quantification of Fas and FasL mRNA levels in lymphocytes: Studies on centenarians

Mechanisms of Ageing and Development

Volumn 124, Issue 4, 2003, Pages 511-516

  Pinti, Marcello ^a   Troiano, Leonarda ^a   Nasi, Milena ^a   Monterastelli, Elena ^a   Moretti, Laura ^a   Bellodi, Cristian ^a   Mazzacani, Annalisa ^a   Mussi, Chiara ^a   Salvioli, Gianfranco ^a   Cossarizza, Andrea ^a  

^a UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

Author keywords

Centenarians; Fas; FasL; Longevity; Real time PCR

Indexed keywords

FAS ANTIGEN; FAS LIGAND; MESSENGER RNA;

AGED; AGING; APOPTOSIS; ASSAY; CELL DEATH; CONFERENCE PAPER; DONOR; GENE CONTROL; GROUPS BY AGE; HOMEOSTASIS; HUMAN; IMMUNE SYSTEM; IMMUNOCOMPETENT CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REGULATORY MECHANISM; SENESCENCE; T LYMPHOCYTE;

EID: 0037396244     PISSN: 00476374     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0047-6374(03)00029-0     Document Type: Conference Paper

References (39)

1. Aggarwal S., Gupta S. Increased apoptosis of T cell subsets in aging humans: altered expression of Fas (CD95), Fas ligand, Bcl-2, and Bax. J. Immunol. 160:1998;1627-1637.
2. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:2000;169-193.
3. Campisi J. Replicative senescence: an old lives' tale? Cell. 84:1996;497-500.
4. Cory S. Fascinating death factor. Nature. 367:1994;317-318.
5. Cohen J.J., Duke R.C. Apoptosis and programmed cell death in immunity. Annu. Rev. Immunol. 10:1992;267-293.
6. Cossarizza A., Ortolani C., Monti D., Franceschi C. Cytometric analysis of immunosenescence. Cytometry. 27:1997;297-313.
7. Cossarizza A., Stent G., Mussini C., Paganelli R., Borghi V., Nuzzo C., Pinti M., Pedrazzi J., Benatti F., Esposito R., Røsok B., Nagata S., Vella S., Franceschi C., De Rienzo B. Deregulation of the CD95/CD95L system in lymphocytes from patients with primary, acute HIV infection. AIDS. 14:2000;345-355.
8. Depraetere V., Golstein P. Fas and other cell death signaling pathways. Sem. Immunol. 9:1997;93-107.
9. Fagnoni F.F., Vescovini R., Passeri G., Bologna G., Pedrazzoni M., Lavagetto G., Casti A., Franceschi C., Passeri M., Sansoni P. Shortage of circulating naive CD8+ T cells provides new insights on immunodeficiency in aging. Blood. 95:2000;2860-2868.
10. Franceschi C., Monti D., Sansoni P., Cossarizza A. The immunology of exceptional individuals: the lesson of centenarians. Immunol. Today. 16:1995;12-16.
11. Fu X., Menke J.G., Chen Y., Zhou G., MacNaul K.L., Wright S.D., Sparrow C.P., Lund E.G. 27-Hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells. J. Biol. Chem. 276:2001;38378-38387.
12. Gupta S. Molecular and biochemical pathways of apoptosis in lymphocytes from aged humans. Vaccine. 18:2000;1596-1601.
13. Golstein P., Ojcius D.M., Young J.D.-E. Cell death mechanisms and the immune system. Immunol. Rev. 121:1991;29-65.
14. Gut M., Leutenegger C.M., Huder J.B., Pedersen N.C., Lutz H. One tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses. J. Virol. Methods. 77:1999;37-46.
15. Itoh N., Yonehara S., Ishii A., Yonehara M., Mizushima S.I., Sameshima M., Hase A., Seto Y., Nagata S. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 66:1991;233-243.
16. Ju S.T., Panka D.J., Cul H., Ettinger R., El-Khatib M., Sherr D.H., Stanger B.Z., Marshak-Rothstein A. Fas(CD95)/FasL interaction required for programmed cell death after T-cell activation. Nature. 373:1995;444-448.
17. Kaplan D., Smith D. Enzymatic amplification staining for flow cytometric analysis of cell surface molecules. Cytometry. 40:2000;81-85.
18. Kappler J.W., Wade T., White J., Kushnir E., Blackman M., Bill J., Roehm N., Marrack P. A T cell receptor Vbeta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 49:1987;263-271.
19. Kreuzer K.A., Lass U., Landt O., Nitsche A., Laser J., Ellerbrok H., Pauli G., Huhn D., Schmidt C.A. Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference. Clin. Chem. 45:1999;297-300.
20. Lenardo M., Chan K.M., Hornung F., McFarland H., Siegel R., Wang J., Zheng L. Mature T lymphocyte apoptosis-immune regulation in a dynamic and unpredictable antigenic environment. Annu. Rev. Immunol. 17:1999;221-253.
21. Liu C., Cheng J., Mountz J.D. Differential expression of human Fas mRNA species upon peripheral blood mononuclear cell activation. Biochem. J. 310:1995;957-963.
22. Lynch D.H., Ramsdell F., Alderson M.R. Fas and FasL in the homeostatic regulation of immune responses. Immunol. Today. 16:1995;569-574.
23. MacDonald H.R., Schneider R., Lees R.K., Howe R.C., Acha-Orbea H., Festenstein H., Zinkernagel R.M., Hengartner H. T cell receptor Vbeta use predicts reactivity and tolerance to Mlsa-encoded antigens. Nature. 332:1988;40-45.
24. Monti D., Salvioli S., Capri M., Malorni W., Straface E., Cossarizza A., Botti B., Piacentini M., Baggio G., Barbi C., Valensin S., Bonafè M., Franceschi C. Decreased susceptibility to oxidative stress-induced apoptosis of peripheral blood mononuclear cells from healthy elderly and centenarians. Mech. Ageing Dev. 121:2000;239-250.
25. Nagata S. Fas ligand-induced apoptosis. Annu. Rev. Genet. 33:1999;29-55.
26. Nagata S., Golstein P. The Fas death factor. Science. 267:1995;1449-1456.
27. Peter M.E., Krammer P.H. Mechanisms of CD95 (APO-1/Fas)-mediated apoptosis. Curr. Opin. Immunol. 10:1998;545-551.
28. Pawelec G., Remarque E., Barnett Y., Solana R. T cells and aging. Front. Biosci. 3:1998;59-99.
29. Phelouzat M.A., Arbogast A., Laforge T., Quadri R.A., Proust J.J. Excessive apoptosis of mature T lymphocytes is a characteristic feature of human immune senescence. Mech. Ageing Dev. 88:1996;25-38.
30. Pinti M., Troiano L., Nasi M., Moretti L., Monterastelli E., Mazzacani A., Mussi C., Ventura P., Olivieri F., Franceschi C., Salvioli G., Cossarizza A. Genetic polymorphisms of Fas (CD95) and FasL (CD178) in human longevity: studies on centenarians. Cell Death Differ. 9:2002;431-438.
31. Potestio M., Pawelec G., Di Lorenzo G., Candore G., D'Anna C., Gervasi F., Lio D., Tranchida G., Caruso C., Romano G.C. Age-related changes in the expression of CD95 (APO1/FAS) on blood lymphocytes. Exp. Gerontol. 34:1999;659-673.
32. Raff M.C. Social controls on cell survival and cell death. Nature. 356:1992;397-400.
33. Sansoni P., Cossarizza A., Brianti V., Fagnoni F., Snelli G., Monti D., Marcato A., Passeri G., Ortolani C., Forti E., Fagiolo U., Passeri M., Franceschi C. Lymphocyte subsets and natural killer cell activity in healthy old people and centenarians. Blood. 80:1993;2767-2773.
34. Schindowski K., Frohlich L., Maurer K., Muller W.E., Eckert A. Age-related impairment of human T lymphocytes' activation: specific differences between CD4(+) and CD8(+) subsets. Mech. Ageing Dev. 123:2002;375-390.
35. Smith D., Sieg S., Kaplan D. Technical note: aberrant detection of cell surface Fas ligand with anti-peptide antibodies. J. Immunol. 160:1998;4159-4160.
36. Thomson C.B. Apoptosis in the pathogenesis and treatment of disease. Science. 267:1995;1456-1462.
37. Wang E. Senescent human fibroblasts resist programmed cell death, and failure to suppress bcl2 is involved. Cancer Res. 55:1995;2284-2292.
38. Winer J., Jung C.K., Shackel I., Williams P.M. Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal. Biochem. 270:1999;41-49.
39. Zhang Y., Herman B. Ageing and apoptosis. Mech. Ageing Dev. 123:2002;245-260.