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Volumn 45, Issue 2, 1999, Pages 297-300

Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of β-actin transcripts as quantitative reference

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; COMPLEMENTARY DNA; DNA POLYMERASE; PRIMER DNA;

EID: 0032990176     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/45.2.297     Document Type: Article
Times cited : (172)

References (7)
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    • Naora, H.1    Naora, H.2
  • 2
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    • Evolution of the functional human beta-actin gene and its multi-pseudogene family: Conservation of noncoding regions and chromosomal dispersion of pseudogenes
    • Ng SY, Gunning P, Eddy R, Ponte P, Leavitt J, Shows T, Kede L. Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes. Mol Cell Biol 1985;5:2720-32.
    • (1985) Mol Celt Biol , vol.5 , pp. 2720-2732
    • Ng, S.Y.1    Gunning, P.2    Eddy, R.3    Ponte, P.4    Leavitt, J.5    Shows, T.6    Kede, L.7
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    • Control genes for reverse-transcription-polymerase chain reaction: A comparison of beta actin and glyceraldehyde phosphate dehydrogenase [letter]
    • Soutar RL, Dillon J, Ralston SH. Control genes for reverse-transcription-polymerase chain reaction: a comparison of beta actin and glyceraldehyde phosphate dehydrogenase [Letter]. Br J Haematol 1997;97:247-8.
    • (1997) Br J Haematol , vol.97 , pp. 247-248
    • Soutar, R.L.1    Dillon, J.2    Ralston, S.H.3
  • 4
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    • Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
    • Livak KJ, Flood JA, Marmaro J, Giusti W, Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 1995;4:357-62.
    • (1995) PCR Methods Appl , vol.4 , pp. 357-362
    • Livak, K.J.1    Flood, J.A.2    Marmaro, J.3    Giusti, W.4    Deetz, K.5
  • 5
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    • Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
    • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-9.
    • (1987) Anal Biochem , vol.162 , pp. 156-159
    • Chomczynski, P.1    Sacchi, N.2
  • 6
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    • Design and testing of β-actin primers rot RT-PCR that do not co-amplify processed pseudogenes
    • Raff T, Van der Giet M, Endemann D, Wiederholt T, Paul M. Design and testing of β-actin primers rot RT-PCR that do not co-amplify processed pseudogenes. Biotechniques 1997;23:456-60.
    • (1997) Biotechniques , vol.23 , pp. 456-460
    • Raff, T.1    Van der Giet, M.2    Endemann, D.3    Wiederholt, T.4    Paul, M.5
  • 7
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    • Limitations and modifications of quantitative polymerase chain reaction. Application to measurement of multiple mRNAs present in small amounts of sample RNA
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.