One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses

Journal of Virological Methods

Volumn 77, Issue 1, 1999, Pages 37-46

  Gut, Marco ^a   Leutenegger, Christian M ^a   Huder, Jon B ^b   Pedersen, Niels C ^c   Lutz, Hans ^a  

^a VETSUISSE FACULTY UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

FCoV; Fluorogenic 5' nuclease assay; One tube RT PCR; Quantitation; TaqMan(TM); Viral RNA

Indexed keywords

EXONUCLEASE; RNA DIRECTED DNA POLYMERASE; VIRUS RNA;

ARTICLE; CORONAVIRUS; FLUORESCENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; VIRUS DETECTION;

ANIMALS; BASE SEQUENCE; CAT DISEASES; CATS; CORONAVIRUS; CORONAVIRUS INFECTIONS; DNA PRIMERS; DNA PROBES; DNA, COMPLEMENTARY; DOGS; EXODEOXYRIBONUCLEASES; FLUORESCENT DYES; HUMANS; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE;

CANINE CORONAVIRUS; CORONAVIRUS; FELIDAE; FELINE CORONAVIRUS; PORCINE RESPIRATORY CORONAVIRUS; SUIDAE; THERMUS THERMOPHILUS; TRANSMISSIBLE GASTROENTERITIS VIRUS;

EID: 0032906092     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(98)00129-3     Document Type: Article

References (19)

1. Addie D.D., Jarrett O. A study of naturally occurring feline coronavirus infections in kittens. Vet. Rec. 130(7):1992;133-137.
2. Birnboim H.C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7(6):1979;1513-1523.
3. Bridgen A., Duarte M., Tobler K., Laude H., Ackermann M. Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus. J. Gen. Virol. 74(Pt 9):1993;1795-1804.
4. Dretzer G., Bellard M., Sassone-Corsi P., Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal. Biochem. 112(2):1981;295-298.
5. Fehr D., Holznagel E., Bolla S., Hauser B., Herrewegh A.A.P.M., Horziner M.C., Lutz H. Placebo-controlled evaluation of a modified life virus vaccine against feline infectious peritonitis: safety and efficacy under field conditions. Vaccine. 15(10):1997;1101-1109.
6. Foley J.E., Poland A., Carlson J., Pedersen N.C. Patterns of feline coronavirus infection and faecal shedding from cats in multiple-cat environments. J. Am. Vet. Med. Assoc. 210(9):1997;1307-1312.
7. Förster V.T. Zwischenmolekulare Energiewanderung und Fluoreszenz. Ann. Phy. 2:1948;55-57.
8. Gamble D.A., Lobbiani A., Gramegna M., Moore L.E., Colucci G. Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens. J. Clin. Microbiol. 35(3):1997;673-675.
9. Herrewegh A.A.P.M., de Groot R.J., Cepica A., Egberink H.F., Horzinek M.C., Rottier P.J. Detection of feline coronavirus RNA in faeces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR. J. Clin. Microbiol. 33(3):1995;684-689.
10. Herrewegh, A.A.P.M., Vennema, H., Horzinek, M.C., Rottier, P.J.M., de Groot, R.J. (unpublished data).
11. Kinoshita T., Imamura J., Nagai H., Shimotohno K. Quantification of gene expression over a wide range by the polymerase chain reaction. Anal. Biochem. 206(2):1992;231-235.
12. Li X., Scott F.W. Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction. Vet. Microbiol. 42(1):1994;65-77.
13. Livak, K., Marmaro, J., Flood, S., Elmer, P., December 1995, Guidelines for designing TaqMan™ fluorogenic probes for 5′ nuclease assays, Perkin Elmer applied biosystems research news.
14. Nagano M., Kelly P.A. Tissue distribution and regulation of rat prolactin receptor gene expression; quantitative analysis by polymerase chain reaction. J. Biol. Chem. 269(18):1994;13337-13345.
15. Pedersen N.C. An overview of feline enteric coronavirus and infectious peritonitis virus infections. Feline Pract. 23:1995;7-22.
16. Pedersen N.C., Boyle J.F., Floyd K. Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture. Am. J. Vet. Res. 42(3):1981;363-367.
17. Singh, M., Tobler, K., Ackermann, M. A novel internal ORF product expressed from a polycistronic mRNA of porcine epidemic diarrhoea virus does not contribute to viral virulence (submitted for publication).
18. Vennema H., Poland A., Hawkins K.F., Pedersen N.C. A comparison of the genomes of FECVs and FIPVs and what they tell us about the relationships between feline coronaviruses and their evolution. Feline Prac. 23(3):1995;40-44.
19. Wang A.M., Doyle M.V., Mark D.F. Quantitation of mRNA by the polymerase chain reaction (published erratum appears in Proc. Natl. Acad. Sci. USA, 1990 April 87 (7), 2865). Proc. Natl. Acad. Sci. USA. 86(24):1989;9717-9721.