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This paper proposes distinct stages of NK cell development based on phenotypic (differential expression of adhesion molecules) and functional properties. In addition, bone marrow NK cells are shown to undergo a phase of intense proliferation.
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Kim S., Iizuka K., Kang H.-S.P., Dokun A., French A.R., Greco D., Yokoyama W.M. In vivo developmental stages in murine natural killer cell maturation. Nat. Immunol. 3:2002;523-528 This paper proposes distinct stages of NK cell development based on phenotypic (differential expression of adhesion molecules) and functional properties. In addition, bone marrow NK cells are shown to undergo a phase of intense proliferation.
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Kim, S.1
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NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules
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Corral L., Hanke T., Vance R.E., Cado D., Raulet D.H. NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules. Eur. J. Immunol. 30:2000;920-930.
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Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)
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Vance R.E., Kraft J.R., Altman J.D., Jensen P.E., Raulet D.H. Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b). J. Exp. Med. 188:1998;1841-1848.
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Idris A.H., Smith H.R.C., Mason L.H., Ortaldo J., Scalzo A.A., Yokoyama W.M. The natural killer gene complex genetic locus Chok encodes Ly-49D, a target recognition receptor that activates natural killing. Proc. Natl. Acad. Sci. U.S.A. 96:1999;6330-6335.
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A ligand for the murine NK activation receptor Ly-49D: Activation of tolerized NK cells from β2-microglobulin-deficient mice
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The authors present a demonstration that the Chinese hamster classical MHC class I molecule, Hm1-C4, is a ligand for the murine activating NKR, Ly49D. This explains the killing of the xenogeneic Chinese hamster ovary cell line CHO by murine NK cells through a Ly49D-dependent mechanism [13,14].
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Furukawa H., Iizuka K., Poursine-Laurent J., Shastri N., Yokoyama W.M. A ligand for the murine NK activation receptor Ly-49D: activation of tolerized NK cells from β2-microglobulin-deficient mice. J. Immunol. 169:2002;126-136 The authors present a demonstration that the Chinese hamster classical MHC class I molecule, Hm1-C4, is a ligand for the murine activating NKR, Ly49D. This explains the killing of the xenogeneic Chinese hamster ovary cell line CHO by murine NK cells through a Ly49D-dependent mechanism [13,14].
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Direct recognition of cytomegalovirus by activating and inhibitory NK cell receptors
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Using an elegant reporter gene assay, this paper demonstrates the interaction of a virus-encoded molecule with an activating NK cell receptor. The MHC-like protein m157 encoded by MCMV binds to the activating receptor Ly49H. Indeed, Ly49H expression (e.g. in C57BL/6 mice) correlates with low virus titers in the spleens of MCMV-infected mice. In certain mouse strains, Ly49H is not expressed (e.g. BALB/c) or m157 binds to an allelic form of the inhibitory NK cell receptor Ly49I (e.g. 129/J). These latter cases correlate with high MCMV titers in infected mice.
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Arase H., Mocarski E.S., Campbell A.E., Hill A.B., Lanier L.L. Direct recognition of cytomegalovirus by activating and inhibitory NK cell receptors. Science. 296:2002;1323-1326 Using an elegant reporter gene assay, this paper demonstrates the interaction of a virus-encoded molecule with an activating NK cell receptor. The MHC-like protein m157 encoded by MCMV binds to the activating receptor Ly49H. Indeed, Ly49H expression (e.g. in C57BL/6 mice) correlates with low virus titers in the spleens of MCMV-infected mice. In certain mouse strains, Ly49H is not expressed (e.g. BALB/c) or m157 binds to an allelic form of the inhibitory NK cell receptor Ly49I (e.g. 129/J). These latter cases correlate with high MCMV titers in infected mice.
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Recognition of a virus-encoded ligand by a natural killer cell activation receptor
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••], this paper demonstrates that m157 is a ligand for Ly49H. Furthermore, it shows that in vivo Ly49H is downmodulated upon ligand encounter.
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••], this paper demonstrates that m157 is a ligand for Ly49H. Furthermore, it shows that in vivo Ly49H is downmodulated upon ligand encounter.
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Makrigiannis A.P., Pau A.T., Schwartzenberg P.L., McVicar D.W., Beck T.W., Anderson S.K. A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice. Genomics. 79:2002;437-444.
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Human natural killer cells with polyclonal lectin and immunoglobulinlike receptors develop from single hematopoietic stem cells with preferential expression of NKG2A and KIR2DL2/L3/S2
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- progenitor cells require IL-15 (or IL-2) and contact with a murine fetal liver cell line to acquire CD94 and KIR expression. Single precursors give rise to NK cells with a diverse receptor repertoire, whereby certain KIRs (such as KIR2DL2/L3/S2) are over-represented.
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- progenitor cells require IL-15 (or IL-2) and contact with a murine fetal liver cell line to acquire CD94 and KIR expression. Single precursors give rise to NK cells with a diverse receptor repertoire, whereby certain KIRs (such as KIR2DL2/L3/S2) are over-represented.
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A demonstration that expressed KIR genes are demethylated, whereas silenced ones are methylated. Importantly, demethylation in vitro, using 5-azacytidine, results in de novo KIR gene expression.
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The selective downregulation of class I major histocompatibility complex proteins by HIV-1 protects HIV-infected cells from NK cells
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•] analyze the adaptation of the Ly49 receptor repertoire in mice transgenic for Ly49C and/or Ly49A. They show that MHC class I engagement by a transgenic Ly49 receptor limits the expression of endogenous Ly49 receptors, irrespective of their MHC class I specificity. These findings are in agreement with sequential receptor acquisition and continuous adaptation to self-MHC class I, which, as one consequence, limits further receptor acquisition.
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•] analyze the adaptation of the Ly49 receptor repertoire in mice transgenic for Ly49C and/or Ly49A. They show that MHC class I engagement by a transgenic Ly49 receptor limits the expression of endogenous Ly49 receptors, irrespective of their MHC class I specificity. These findings are in agreement with sequential receptor acquisition and continuous adaptation to self-MHC class I, which, as one consequence, limits further receptor acquisition.
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