Functional characterization of Glu298Asp mutant human endothelial nitric oxide synthase purified from a yeast expression system

Nitric Oxide - Biology and Chemistry

Volumn 8, Issue 1, 2003, Pages 7-14

  Golser, Regina ^a   Gorren, Antonius C F ^a   Mayer, Bernd ^a   Schmidt, Kurt ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

Biochemical characterization; Endothelial nitric oxide synthase; Expression; Glu298Asp polymorphism; Purification

Indexed keywords

ARGININE; ASPARTIC ACID; CALMODULIN; CYTOCHROME C; GLUTAMIC ACID; HEME; HYDROGEN PEROXIDE; IODINE 125; MUTANT PROTEIN; NITRIC OXIDE SYNTHASE; OXYGEN; QUERCETIN; RADIOLIGAND; TETRAHYDROBIOPTERIN;

ARTICLE; CONTROLLED STUDY; ELECTRON TRANSPORT; ENDOTHELIUM; ENZYME ACTIVATION; NONHUMAN; OXIDATION; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; WILD TYPE; YEAST;

ASPARTIC ACID; CALMODULIN; COENZYMES; DIMERIZATION; ENDOTHELIUM; GENE EXPRESSION; GLUTAMIC ACID; HUMANS; HYDROGEN PEROXIDE; KINETICS; MUTATION, MISSENSE; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE III; PICHIA; POLYMORPHISM, GENETIC; RECOMBINANT PROTEINS;

PICHIA; PICHIA PASTORIS;

EID: 0037331863     PISSN: 10898603     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1089-8603(02)00131-3     Document Type: Article

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