Characterization of heme-deficient neuronal nitric-oxide synthase reveals a role for heme in subunit dimerization and binding of the amino acid substrate and tetrahydrobiopterin

Journal of Biological Chemistry

Volumn 271, Issue 13, 1996, Pages 7336-7342

  Klatt, Peter ^a   Pfeiffer, Silvia ^a   List, Barbara M ^a   Lehner, Dieter ^a   Glatter, Otto ^a   Bächinger, Hans Peter ^b   Werner, Ernst R ^c   Schmidt, Kurt ^a   Mayer, Bernd ^a  

^a UNIVERSITY OF GRAZ   (Austria)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGININE; CITRULLINE; CYTOCHROME; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; HEME; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; OXIDOREDUCTASE; OXYGEN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; SUPEROXIDE; TETRAHYDROBIOPTERIN;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CIRCULAR DICHROISM; DIMERIZATION; ELECTRON TRANSPORT; ENZYME ACTIVITY; FLUORESCENCE; INSECT; NERVE CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; RAT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ANIMALS; ARGININE; BINDING SITES; BIOPTERIN; BRAIN; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; CITRULLINE; FLAVIN MONONUCLEOTIDE; FLAVIN-ADENINE DINUCLEOTIDE; HEME; ISOENZYMES; LIGHT; MACROMOLECULAR SUBSTANCES; NEURONS; NITRIC OXIDE SYNTHASE; NITROARGININE; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RATS; SCATTERING, RADIATION; THERMODYNAMICS;

EID: 0029929385     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.13.7336     Document Type: Article

References (53)

1. Bredt, D. S., and Snyder, S. H. (1994) Annu. Rev. Biochem. 63, 175-195
2. Nathan, C. (1992) FASEB J. 6, 3051-3064
3. Garthwaite, J., and Boulton, C. L. (1995) Annu. Rev. Physiol. 57, 683-706
4. Gross, S. S., and Wolin, M. S. (1995) Annu. Rev. Physiol. 57, 737-769
5. Bredt, D. S., and Snyder, S. H. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 682-685
6. Mayer, B., John, M., and Böhme, E. (1990) FEBS Lett. 277, 215-219
7. Schmidt, H. H. H. W., Pollock, J. S., Nakane, M., Gorsky, L. D., Förstermann, U., and Murad, F. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 365-369
8. Mayer, B., John, M., Heinzel, B., Werner, R., Wachter, H., Schultz, G., and Böhme, E. (1991) FEBS Lett. 288, 187-191
9. Stuehr, D. J., and Ikeda-Saito, M. (1992) J. Biol. Chem. 267, 20547-20550
10. Bredt, D. S., Ferris, C. D., and Snyder, S. H. (1992) J. Biol. Chem. 267, 10976-10981
11. Schmidt, H. H. H. W., Smith, R. M., Nakane, M., and Murad, F. (1992) Biochemistry 31, 3243-3249
12. McMillan, K., Bredt, D. S., Hirsch, D. J., Snyder, S. H., Clark, J. E., and Masters, B. S. S. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 11141-11145
13. Klatt, P., Schmidt, K., and Mayer, B. (1992) Biochem. J. 288, 15-17
14. Klatt, P., Schmidt, K., Lehner, D., Glatter, O., Bächinger, H. P., and Mayer, B. (1995) EMBO J. 14, 3687-3695
15. Bredt, D. S., Hwang, P. M., Glatt, C. E., Lowenstein, C., Reed, R. R., and Snyder, S. H. (1991) Nature 351, 714-718
16. Renaud, J. P., Boucher, J. L., Vadon, S., Delaforge, M., and Mansuy, D. (1993) Biochem. Biophys. Res. Commun. 192, 53-60
17. Degtyarenko, K. N., and Archakov, A. I. (1993) FEBS Lett. 332, 1-8
18. Klatt, P., Heinzel, B., John, M., Kastner, M., Böhme, E., and Mayer, B. (1992) J. Biol. Chem. 267, 11374-11378
19. Richards, M. K., and Marletta, M. A. (1994) Biochemistry 33, 14723-14732
20. McMillan, K., and Masters, B. S. S. (1995) Biochemistry 34, 3686-3693
21. Wang, J. L., Stuehr, D. J., Ikeda-Saito, M., and Rousseau, D. L. (1993) J. Biol. Chem. 268, 22255-22258
22. Sheta, E. A., Mc Millan, K., and Masters, B. S. S. (1994) J. Biol. Chem. 269, 15147-15153
23. Ghosh, D. K., and Stuehr, D. J. (1995) Biochemistry 34, 801-807
24. Abu-Soud, H. M., and Stuehr, D. J. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 10769-10772
25. Abu-Soud, H. M., Feldman, P. L., Clark, P., and Stuehr, D. J. (1994) J. BioL Chem. 269, 32318-32326
26. Nishimura, J. S., Martasek, P., McMillan, K., Salerno, J. C., Lui, Q., Gross, S. S., and Masters, B. S. S. (1995) Biochem. Biophys. Res. Commun. 210, 288-294
27. Stuehr, D. J., Kwon, N. S., Nathan, C. F., Griffith, O. W., Feldman, P. L., and Wiseman, J. (1991) J. Biol. Chem. 266, 6259-6263
28. Klatt, P., Schmidt, K., Uray, G., and Mayer, B. (1993) J. Biol. Chem. 268, 14781-14787
29. Baek, K. J., Thiel, B. A., Lucas, S., and Stuehr, D. J. (1993) J. Biol. Chem. 268, 21120-21129
30. Werner, E. R., Schmid, M., Werner-Felmayer, G., Mayer, B., and Wachter, H. (1994) Biochem. J. 304, 189-193
31. Klatt, P., Heinzel, B., John, M., Kastner, M., Böhme, E., and Mayer, B. (1992) J. Biol. Chem. 267, 11374-11378
32. Harteneck, C., Klatt, P., Schmidt, K., and Mayer, B. (1994) Biochem. J. 304, 683-686
33. Mayer, B., Klatt, P., List, B. M., Harteneck, C., and Schmidt, K. (1995) Methods Ensymol., in press
34. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
35. Klatt, P., Schmidt, K., Werner, E. R., and Mayer, B. (1996) Methods Enzymol., in press
36. Mayer, B., Klatt, P., Werner, E. R., and Schmidt, K. (1994) Neuropharmacology 33, 1253-1259
37. DeLevie, R. (1986) J. Chem. Educ. 63, 10-15
38. Klatt, P., Schmid, M., Leopold, E., Schmidt, K., Werner, E. R., and Mayer, B. (1994) J. Biol. Chem. 269, 13861-13866
39. Klatt, P., Schmidt, K., Brunner, F., and Mayer, B. (1994) J. Biol. Chem. 269, 1674-1680
40. Brunner, F., and Kukovetz, W. R. (1991) Br. J. Pharmacol. 102, 373-380
41. Laemmli, U. K. (1970) Nature 227, 680-685
42. Thomas, P. E., Ryan, D., and Levin, W. (1976) Anal. Biochem. 75, 168-176
43. Chu, B. (1991) Laser Light Scattering, 2nd Ed, Academic Press, New York
44. Huglin, M. B. (1972) Light Scattering from Polymer Solutions, Academic Press, New York
45. Compton, L. A., Davis, J. M., MacDonald, J. R., and Bächinger, H. P. (1992) Eur. J. Biochem. 206, 927-934
46. Compton, L. A., Mathews, C. K., and Johnson, W. C., Jr. (1987) J. Biol. Chem. 262, 13039-13043
47. Pinnix, I. B., Guzman, G., Bonkovsky, H. L., Zaki, S. R., and Kinkade, J. M. (1994) Arch. Biochem. Biophys. 312, 447-458
48. McMillan, K., and Masters, B. S. S. (1993) Biochemistry 32, 9875-9880
49. Matsuoka, A., Stuehr, D. J., Olson, J. S., Clark, P., and Ikeda-Saito, M. (1994) J. Biol. Chem. 269, 20335-20339
50. Abu-Soud, H. M., Yoho, L. L., and Stuehr, D. J. (1994) J. Biol. Chem. 269, 32047-32050
51. Wang, J. L., Stuehr, D. J., and Rousseau, D. L. (1995) Biochemistry 34, 7080-7087
52. Heinzel, B., John, M., Klatt, P., Böhme, E., and Mayer, B. (1992) Biochem. J. 281, 627-630
53. Pou, S., Pou, W. S., Bredt, D. S., Snyder, S. H., and Rosen, G. M. (1992) J. Biol. Chem. 267, 24173-24176