Proliferation of intracellular structure corresponding to reduced affinity of fluconazole for cytochrome P-450 in two low-susceptibility strains of Candida albicans isolated from a Japanese AIDS patient

Microbiology and Immunology

Volumn 47, Issue 2, 2003, Pages 117-124

  Maebashi, Kazunori ^a,b   Kudoh, Michinari ^a   Nishiyama, Yayoi ^a   Makimura, Koichi ^a   Kamai, Yasuki ^a   Uchida, Katsuhisa ^a   Yamaguchi, Hideyo ^a  

^a TEIKYO UNIVERSITY   (Japan)

^b MEIJI SEIKA KAISHA LTD   (Japan)

Author keywords

Candida albicans; Cytochrome P 450; Electron microscopy; Fluconazole resistance

Indexed keywords

CYTOCHROME P450; FLUCONAZOLE; METHYLTRANSFERASE; STEROL 14ALPHA DEMETHYLASE;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ARTICLE; BINDING AFFINITY; CANDIDA ALBICANS; CELL PROLIFERATION; CONTROLLED STUDY; DRUG SENSITIVITY; DRUG TARGETING; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; FUNGAL STRAIN; FUNGUS ISOLATION; GENE; GENE ERG11; HUMAN; JAPAN; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE;

CANDIDA; CANDIDA ALBICANS; FUNGI; RNA VIRUSES;

EID: 0037284177     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2003.tb02794.x     Document Type: Article

References (36)

1. Albertson, G.D., Niimi, M., Cannon, R.D., and Jenkinson, H.F. 1996. Multiple efflux mechanisms are involved in Candida albicans fluconazole resistance. Antimicrob. Agents Chemother. 40: 2835-2841.
2. Boscott, P.E., and Grant, G.H. 1994. Modeling cytochrome P450 14α-demethylase (Candida albicans) from P450cam. J. Mol. Graph. 12: 185-192.
3. Fling, M.E., Kopf, J., Tamarkin, A., Gorman, J.A., Smith, H.A., and Koltin, Y. 1991. Analysis of a Candida albicans gene that encodes a novel mechanism for resistance to benomyl and methotrexate. Mol. Gen. Genet. 227: 318-329.
4. Franz, R., Kelly, S.L., Lamb, D.C., Kelly, D.E., Ruhnke, M., and Morschhuser, J. 1998. Multiple molecular mechanisms contribute to a stepwise development of fluconazole resistance in clinical Candida albicans strains. Antimicrob. Agents Chemother. 42: 3065-3072.
5. Ghannoum, M.A., Rex, J.H., and Galgiani, J.N. 1996. Susceptibility testing of fungi: current status of correlation of in vitro data with clinical outcome. J. Clin. Microbiol. 34: 489-495.
6. Howell, S.A., Mallet, A.I., and Noble, W.C. 1990. A comparison of the sterol content of multiple isolates of the Candida albicans Darlington strain with other clinically azolesensitive and -resistant strains. J. Appl. Bacteriol. 69: 692-696.
7. 5,6-desaturation. FEBS Lett. 400: 80-82.
8. Lai, M.H., and Kirsch, D.R. 1989. Nucleotide sequence of cytochrome P450 L1A1 (lanosterol 14α-demethylase) from Candida albicans. Nucleic Acids Res. 17: 804.
9. Lamb, D.C., Kelly, D.E., Schunck, W.H., Shyadehi, A.Z., Akhtar, M., Lowe, D.J., Baldwin, B.C., and Kelly, S.L. 1997. The mutation T315A in Candida albicans sterol 14α-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity. J. Biol. Chem. 272: 5682-5688.
10. Löffler, J., Kelly, S.L., Hebart, H., Schumacher, U., L.-Flörl, C., and Einsele, H. 1997. Molecular analysis of cyp51 from fluconazole-resistant Candida albicans strains. FEMS Microbiol. Lett. 151: 263-268.
11. Maebashi, K., Niimi, M., Kudoh, M., Fischer, F.J., Makimura, K., Nümi, K., Piper, R.J., Uchida, K., Arisawa, M., Cannon, R.D., and Yamaguchi, H. 2001. Mechanisms of fluconazole resistance in Candida albicans isolates from Japanese AIDS patients. J. Antimicrob. Chemother. 47: 527-536.
12. Maebashi, K., Kudoh, M., Nishiyama, Y., Makimura, K., Uchida, K., Mori, T., and Yamaguchi, H. 2002. A novel mechanism of fluconazole resistance associated with fluconazole sequestration in Candida albicans isolates from a myelofibrosis patient. Microbiol. Immunol. 46: 317-326.
13. Marichal, P., Koymans, L., Willemsens, S., Bellens, D., Verhasselt, P., Luyten, W., Borgers, M., Ramaekers, F.C.S., Odds, F.C., and Vanden Bossche, H. 1999. Contribution of mutations in the cytochrome P450 14α-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans. Microbiology 145: 2701-2713.
14. National Committee for Clinical Laboratory Standards. 1997. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. NCCLS document M27-A. National Committee for Clinical Laboratory Standards, Villanova, Pa.
15. Odds, F.C. 1996. Resistance of clinically important yeasts to antifungal agents. Int. J. Antimicrob. Agents 6: 145-147.
16. Ohkuma, M., Park, S.M., Zimmer, T., Menzel, R., Vogel, F., Schunk, W.H., Ohta, A., and Takagi, M. 1995. Proliferation of intracellular membrane structures upon homologous overproduction of cytochrome P-450 in Candida maltosa. Biochim. Biophys. Acta 1236: 163-169.
17. Oka, S. 1994. Fungal infections in AIDS patients. Jpn. J. Med. Mycol. 35: 241-245.
18. Omura, T., and Sato, R. 1961. The carbon monoxide-binding pigment of liver microsomes. 1. Evidence for its haemoprotein nature. J. Biol. Chem. 239: 2370-2378.
19. Perea, S., Lopez-Ribot, J.L., Kirkpatrick, W.R., McAtee, R.K., Santillan, R.A., Martinez, M., Calabrese, D., Sanglard, D., and Patterson, T.F. 2001. Prevalence of molecular mechanisms of resistance to azole antifungal agents in Candida albicans strains displaying high-level fluconazole resistance isolated from human immunodeficiency virus-infected patients. Antimicrob. Agents Chemother. 45: 2676-2684.
20. Prasad, R., Wergifosse, P.De, Goffeau, A., and Balzi, E. 1995. Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals. Curr. Genet. 27: 320-329.
21. Rex, J.H., Rinaldi, M.G., and Pfaller, M.A. 1995. Resistance of Candida species to fluconazole. Antimicrob. Agents Chemother. 39: 1-8.
22. Ryley, J.F., Wilson, R.G., and Barrett-Bee, K.J. 1984. Azole resistance in Candida albicans. Sabouraudia 22: 53-63.
23. Sanglard, D., Kuchler, K., Ischer, F., Pagani, J.L., Monod, M., and Bille, J. 1995. Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters. Antimicrob. Agents Chemother. 39: 2378-2386.
24. Sanglard, D., Ischer, F., Monod, M., and Bille, J. 1997. Cloning of Candida albicans genes conferring resistance of azole antifungal agents: characterization of CDR2, a new multidrug ABC transporter gene. Microbiology 143: 405-416.
25. Sanglard, D., Ischer, F., Koymans, L., and Bille, J. 1998. Amino acid substitutions in the cytochrome P-450 lanosterol 14α-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents. Antimicrob. Agents Chemother. 42: 241-253.
26. Schunck, W.H., Vogel, F., Gross, B., Kärgel, E., Mauersberger, S., Köpke, K., Gengnagel, C., and Müller, H.G. 1991. Comparison of two cytochromes P-450 from Candida maltosa: primary structures, substrate specificities and effects of their expression in Saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum. Eur. J. Cell Biol. 55: 336-345.
27. Supply, P., Wach, A., Sempoux-T.D., and Goffeau, A. 1993. Proliferation of intracellular structures upon overexpression of the PMA2 ATPase in Saccharomyces cerevisiae. J. Biol. Chem. 268: 19744-19752.
28. Vanden Bossche, H., Marichal, P., Gorrens, J., Bellens, D., Moereels, H., and Janssen, P.A. 1990. Mutation in cytochrome P-450-dependent 14α-demethylase results in decreased affinity for azole antifungals. Biochem. Soc. Trans. 18: 56-59.
29. Vanden Bossche, H., Marichal, P., and Odds, F.C. 1994. Molecular mechanisms of drug resistance in fungi. Trends Microbiol. 2: 393-400.
30. Venkateswarlu, K., Denning, D.W., Manning, N.J., and Kelly, S.L. 1995. Resistance to fluconazole in Candida albicans from AIDS patients correlated with reduced intracellular accumulation of drug. FEMS Microbiol. Lett. 131: 337-341.
31. Venkateswarlu, K., Denning, D.W., Manning, N.J., and Kelly, S.L. 1996. Comparison of D0870, a new triazole antifungal agent, to fluconazole for inhibition of Candida albicans cytochrome P-450 by using in vitro assays. Antimicrob. Agents Chemother. 40: 1382-1386.
32. 5 in yeast results in marked proliferation of the intracellular membrane. J. Cell Sci. 106: 249-259.
33. White, T.C. 1997. Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus. Antimicrob. Agents Chemother. 41: 1482-1487.
34. White, T.C. 1997. The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14α-demethylase in Candida albicans. Antimicrob. Agents Chemother. 41: 1488-1494.
35. White, T.C., Marr, K.A., and Bowden, R.A. 1998. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance. Clin. Microbiol. Rev. 11: 382-402.
36. Wright, R., Basson, M., D'Ari, L., and Rine, J. 1988. Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus. J. Cell Biol. 107: 101-114.