The mutation T315A in Candida albicans sterol 14α-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity

Journal of Biological Chemistry

Volumn 272, Issue 9, 1997, Pages 5682-5688

  Lamb, David C ^a   Kelly, Diane E ^a   Schunck, Wolf Hagen ^b   Shyadehi, Akbar Z ^c   Akhtar, Muhammed ^c   Lowe, David J ^d   Baldwin, Brian C ^e   Kelly, Steven L ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^d JOHN INNES CENTRE   (United Kingdom)

^e JEALOTT S HILL RESEARCH STATION   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DEMETHYLASE; FLUCONAZOLE; KETOCONAZOLE; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; BINDING AFFINITY; CANDIDA ALBICANS; CANDIDIASIS; ENZYME STRUCTURE; GENE MUTATION; HYDROGEN BOND; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIFUNGAL AGENTS; CANDIDA ALBICANS; CYTOCHROME P-450 ENZYME SYSTEM; DRUG RESISTANCE, MICROBIAL; ELECTRON SPIN RESONANCE SPECTROSCOPY; FLUCONAZOLE; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OXIDOREDUCTASES; RATS; RESTRICTION MAPPING; SEQUENCE ALIGNMENT;

CANDIDA ALBICANS; SACCHAROMYCES CEREVISIAE;

EID: 0031054924     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.9.5682     Document Type: Article

References (23)

1. Kalb, V., Woods, C. W., Turi, T. G., Dey, C. R., Sutter, T. R., and Loper J. C. (1987) DNA 6, 528-537
2. Stromstedt, M., Rozman, D., and Waterman, M. R. (1996) Arch. Biochem. Biophys. 329, 73-81
3. Rex, J. H., Rinaldi, M. G., and Pfaller, M. A. (1995) Antimicrob. Agents Chemother. 39, 1-8
4. Kelly, S. L., Arnoldi, A., and Kelly, D. E. (1993) Biochem. Soc. Trans. 21, 1035-1039
5. Venkateswarlu, K., Denning, D. W., Manning, N. J., and Kelly, S. L. (1995) FEMS Lett. 131, 337-341
6. Sanglard, D., Kuchler, K., Ischer, F., Pagani, J.-L., Monod, M., and Bille, J. (1995) Antimicrob. Agents Chemother. 39, 1-8
7. Watson, P. F., Rose, M. E., Ellis, S. W., England, H., and Kelly, S. L. (1989) Biochem. Biophys. Res. Commun. 164, 1170-1175
8. Joseph-Horne, T., Hollomon, D. W., Manning, N. J., and Kelly, S. L. (1995) FEMS Lett. 127, 29-34
9. Joseph-Horne, T., Hollomon, D. W., Loeffler, R. S. T., and Kelly, S. L. (1995) FEBS Lett. 374, 174-178
10. Kelly, S. L., Lamb, D. C., Corran, A. J., Baldwin, B. C., Parks, L. W., and Kelly, D. E. (1995) FEBS Lett. 377, 217-220
11. Boscott, P. E., and Grant, G. H. (1994) J. Mol. Graph. 12, 185-193
12. Shyadehi, A. Z., Lamb, D. C., Kelly, S. L., Kelly, D. E., Schunck, W.-H., Wright, J. N., Corina, D., and Akhtar, M. (1996) J. Biol. Chem. 271, 12445-12450
13. Omura, T., and Sato, R. (1964) J. Biol. Chem. 239, 2370-2378
14. Lowry, O. H., Rosebrough, N. J., Farr, A. O., and Randall, R. J. (1951) J. Biol. Chem. 193, 265-275
15. Santos, M. A. S., Keith, G., and Tuite, M. F. (1993) EMBO J. 12, 607-616
16. Yoshida, Y., and Aoyama, Y. (1984) J. Biol. Chem. 259, 1655-1660
17. Jefcoate, C. R., Gaylor, J. L., and Calabrese, R. L. (1969) Biochemistry 8, 3455-3463
18. Hitchcock, C. A., Dickinson, K., Brown, S. B., Evans, G. V., and Adams, D. J. (1990) Biochem. J. 266, 475-480
19. Kirsch, D. R., Lai, M. H., and O'Sullivan, J. (1988) Gene (Amst.) 68, 229-237
20. Aoyama, Y., Funae, Y., Noshiro, M., Horiuchi, T., and Yoshida, Y. (1994) Biochem. Biophys. Res. Commun. 201, 1320-1326
21. Hasemann, C. A., Kurumbail, R. G., Boddupalli, S. S., Peterson, J. A., and Deisenhofer, J. (1995) Structure 2, 41-62
22. Baily, G. G., Perry, F. M., Denning, D. W., and Mandal, B. K. (1994) AIDS 8, 787-792
23. Lamb, D. C., Corran, A., Baldwin, B. C., Kwon-Chung, J., and Kelly, S. L. (1995) FEBS Lett. 368, 326-330