Removal of antibiotic resistance genes from transgenic tobacco plastids

Nature Biotechnology

Volumn 18, Issue 11, 2000, Pages 1172-1176

  Iamtham, S ^a   Day, A ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Glufosinate; GM crops; Marker free; Plastid transformation; Tobacco

Indexed keywords

PHOSPHINOTHRICIN; SPECTINOMYCIN; STREPTOMYCIN;

AGRICULTURE; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; DISEASE RESISTANCE; DNA MODIFICATION; DNA RECOMBINATION; DNA TRANSFER; DRUG TOLERANCE; GENE INSERTION; MARKER GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASTID; PRIORITY JOURNAL; TOBACCO; TRANSGENIC PLANT;

AMINOBUTYRIC ACIDS; BLOTTING, SOUTHERN; CYTOPLASM; DRUG RESISTANCE; DRUG RESISTANCE, MICROBIAL; GENES, REPORTER; GENETIC MARKERS; GENOME, PLANT; HERBICIDES; MODELS, GENETIC; NUCLEOTIDYLTRANSFERASES; PHENOTYPE; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; PLASTIDS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SPECTINOMYCIN; STREPTOMYCIN; TOBACCO; TRANSFORMATION, GENETIC;

EMBRYOPHYTA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0033768431     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/81161     Document Type: Article

References (27)

1. Transformation systems for generating marker-free transgenic plants
2. Towards plastid transformation in flowering plants
3. Low probability of chloroplast movement from oilseed rape (Brassica napus) into wild Brassica rapa
4. Containment of herbicide resistance through genetic engineering of the chloroplast genome
5. The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression
6. Chloroplast transformation in Chlamydomonas with high velocity microprojectiles
7. Stable transformation of plastids in higher plants
8. Amplification of a chimeric Bacillus gene in chloroplast leads to an extraordinary level of insecticidal protein in tobacco
9. Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects
10. High-yield production of a human therapeutic protein in tobacco chloroplasts
11. High frequency plastid transformation in tobacco by selection for a chimeric aadA gene
12. Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants
13. Plastid transformation in Arabidopsis thaliana
14. Stable chloroplast transformation in potato: Use of green fluorescent protein as a plastid marker
15. Assaying chimeric genes in plants: The GUS gene fusion system
16. Transgenic expression of aminoglycoside adenine transferase in the chloroplast: A selectable marker for site-directed transformation in Chlamydomonas
17. A cassette containing the BAR gene of Streptomyces hygroscopicus: A selectable marker for plant transformation
18. Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: Mapping of 11 ribosomal protein genes
19. Engineering herbicide resistance by expression of a detoxifying enzyme
20. Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA messenger RNA
21. Efficient targeting of foreign genes into the tobacco plastid genome
22. Selectable marker recycling in the chloroplast
23. Genetic flexibility of plant chloroplasts
24. The partitioning of cytoplasmic organelles at cell division
25. Stable albinism induced without mutagenesis: A model for ribosome-free plastid inheritance
26. Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts