Relation between presence-absence of a visible nucleoid and metabolic activity in bacterioplankton cells

Limnology and Oceanography

Volumn 41, Issue 6, 1996, Pages 1161-1168

  Choi, Joon W ^a   Sherr, Evelyn B ^a   Sherr, Barry F ^a  

^a OREGON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); PROKARYOTA;

EID: 0030462076     PISSN: 00243590     EISSN: None     Source Type: Journal    
DOI: 10.4319/lo.1996.41.6.1161     Document Type: Article

References (18)

1. DAWES, I. W., AND I. W. SUTHERLAND. 1992. Microbial physiology, 2nd ed. Blackwell.
2. DEL GIORGIO, P. A., AND G. SCARBOROUGH. 1995. Increase in the proportion of metabolically active bacteria along gradients of enrichment in freshwater and marine plankton: Implications for estimates of bacterial growth and production. J. Plankton Res. 17: 1905-1924.
3. DOUGLAS, D. J., J. A. NOVITSKY, AND R. O. FOURNIER. 1987. Microautoradiography-based enumeration of bacteria with estimates of thymidine-specific growth and production rates. Mar. Ecol. Prog. Ser. 36: 91-99.
4. GASOL, J. M., P. A. DEL GIORGIO, R. MASSANA, AND C. M. DUARTE. 1995. Active vs. inactive bacteria: Size dependence in a coastal marine plankton community. Mar. Ecol. Prog. Ser. 128: 91-97.
5. KJELLEBERG, S., K. B. G. FLARDH, T. NYSTROM, AND D. W. MORIARTY. 1993. Growth and starvation of bacteria, p. 289-320. In T. E. Ford [ed.], Aquatic microbiology: An ecological approach. Blackwell.
6. LI, W. K. W., J. F. JELLETT, AND P. M. DICKIE. 1995. DNA distributions in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr. 40: 1485-1495.
7. MORITA, R. Y. 1985. Starvation and miniaturization of heterotrophs, with special emphasis on maintenance of the starved viable state, p. 111-130. In M. M. Fletcher and G, D. Floodgate [eds.], Bacteria in their natural environments. Academic.
8. NEWELL, S. Y., R. D. FALLON, B. F. SHERR, AND E. B. SHERR. 1988. Mesoscale temporal variation in bacterial standing crop, percent active cells, productivity and output in a salt-marsh tidal river. Int. Ver. Theor. Angew. Limnol. Verb. 23: 1839-1845.
9. PEDROS-ALIO, C., AND S. Y. NEWELL. 1989. Microautoradiographic study of thymidine uptake in brackish waters around Sapelo Island, Georgia, USA. Mar. Ecol. Prog. Ser. 55: 83-94.
10. PORTER, J., J. DIAPER, C. EDWARDS, AND R. PICKUP. 1995. Direct measurements of natural planktonic bacterial community viability by flow cytometry. Appl. Environ. Microbiol. 61: 2783-2786.
11. PORTER, K. G., AND Y. FEIG. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943-948.
12. REIMANN, B., P. NEELSEN, M. JEPPESEN, B. MARCUSSEN, AND J. A. FUHRMAN. 1984. Diel changes in bacterial biomass and growth rates in coastal marine environments, determined by means of thymidine incorporation into DNA, frequency of dividing cells (FDC), and microradiography. Mar. Ecol. Prog. Ser. 17: 227-235.
13. RODRIGUEZ, G. G., D. PHIPPS, K. ISHIGURO, AND H. F. RIDGWAY. 1992. Use of a fluorescent redox probe for direct visualization of actively respiring bacterial. Appl. Environ. Microbiol. 58: 1801-1808.
14. SHERR, E. B., D. A. CARON, AND B. F. SHERR. 1993. Staining of heterotrophic protists for visualization via epifluorescence microscopy, p. 213-227. In P. F. Kemp et al. [eds.], Handbook of methods in aquatic microbial ecology. Lewis.
15. SIMON, M., AND F. AZAM. 1989. Protein context and protein synthesis rates of planktonic marine bacteria. Mar. Ecol. Prog. Ser. 51:201-213.
16. SUZUKI, M. T., E. B. SHERR, AND B. F. SHERR. 1993. DAPI direct counting underestimates bacterial abundances and average cell size compared to AO direct counting. Limnol. Oceanogr. 38: 1566-1570.
17. TABOR, P. S., AND R. A. NEIHOF. 1982. Improved method for determination of respiring individual microorganisms in natural waters. Appl. Environ. Microbiol. 43: 1249-1255.
18. ZWEIFEL, U. L., AND A. HAGSTRÖM. 1995. Total counts of marine bacteria include a large fraction of non-nucleoid containing "ghosts." Appl. Environ. Microbiol. 61: 2180-2185.