Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures

Applied and Environmental Microbiology

Volumn 65, Issue 6, 1999, Pages 2409-2417

  Sieracki, Michael E ^a   Cucci, Terry L ^a   Nicinski, Justyna ^a,b  

^a BIGELOW LABORATORY FOR OCEAN SCIENCES   (United States)

^b UNIVERSITY OF NEW ENGLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYE; TETRAZOLIUM;

BACTERIOPLANKTON; FLOW CYTOMETRY; RESPIRATION;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; ECOLOGY; FLOW CYTOMETRY; MARINE ENVIRONMENT; NONHUMAN; OXIDATION REDUCTION REACTION; PLANKTON;

BACTERIA (MICROORGANISMS);

EID: 0032976005     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.6.2409-2417.1999     Document Type: Article

References (36)

1. Ammerman, J. W., J. A. Fuhrman. Å. Hagström, and F. Azam. 1984. Bacterioplankton growth in seawater. I. Growth kinetics and cellular characteristics in seawater cultures. Mar. Ecol. Prog. Ser. 18:31-39.
2. Azam, F. 1998. Microbial control of oceanic carbon flux: the plot thickens. Science 280:694-696.
3. Carlson, C. A., and H. W. Ducklow. 1996. Growth of bacterioplankton and consumption of dissolved organic carbon in the Sargasso Sea. Aquat. Microb. Ecol. 10:69-85.
4. Chin, W.-C., M. V. Orellana, and P. Verdugo. 1998. Spontaneous assembly of marine dissolved organic matter into polymer gels. Nature 391:568-572.
5. Cho, B. C., and F. Azam. 1988. Major role of bacteria in biogeochemical fluxes in the ocean's interior. Nature 332:441-443.
6. Choi, J. W., B. F. Sherr, and E. B. Sherr. Dead or alive? A large fraction of ETS-inactive marine bacterioplankton cells, as assessed by reduction of CTC, can become ETS-active with incubation and substrate addition. Aquat. Microb. Ecol., in press.
7. Choi, J. W., E. B. Sherr, and B. F. Sherr. 1996. Relation between presence-absence of a visible nucleoid and metabolic activity in bacterioplankton cells. Limnol. Oceanogr. 41:1161-1168.
8. Cole, J. J., and M. L. Pace. 1995. Why measure bacterial production? A reply to the comment by Jahnke and Craven. Limnol. Oceanogr. 40:441-444.
9. 2 production in mixed microbial communities. Microb. Ecol. 34:237-247.
10. del Giorgio, P. A., Y. T. Prairie, and D. F. Bird. 1997. Coupling between rates of bacterial production and the abundance of metabolically active bacteria in lakes, enumerated using CTC reduction and flow cytometry. Microb. Ecol. 34:144-145.
11. del Giorgio, P. A., and G. Scarborough. 1995. Increase in the proportion of metabolically active bacteria along gradients of enrichment in freshwater and marine plankton: implications for estimates of bacterial growth and production rates. J. Plankton Res. 17:1905-1924.
12. Epstein, S. S., and J. Rössel. 1995. Methodology of in situ grazing experiments: evaluation of a new vital dye for preparation of fluorescently labeled bacteria. Mar. Ecol. Prog. Ser. 128:143-150.
13. Fuhrman, J., and F. Azam. 1982. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: evaluation and field results. Mar. Biol. 66:109-120.
14. Gasol, J. M., P. A. del Giorgio, R. Massana, and C. M. Duarte. 1995. Active versus inactive bacteria: size-dependence in a coastal marine plankton community. Mar. Ecol. Prog. Ser. 128:91-97.
15. Jahnke, R. A., and D. B. Craven. 1995. Quantifying the role of heterotrophic bacteria in the carbon cycle: a need for respiration rate measurements. Limnol. Oceanogr. 40:436-441.
16. Kaprelyants, A. S., and D. B. Kell. 1993. The use of 5-cyano-2,3-ditolyl tetrazolium chloride and flow cytometry for the visualization of respiratory activity in individual cells of Micrococcus luteus. J. Microbiol. Methods 17: 115-122.
17. Karner, M., and J. A. Fuhrman. 1997. Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl. Environ. Microbiol. 63:1208-1213.
18. Kirchman, D. L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria, p. 509-512. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Fla.
19. Krembs, C., A. R. Juhl, R. A. Long, and F. Azam. 1998. Nanoscale patchiness of bacteria in lake water studied with the spatial information preservation method. Limnol. Oceanogr. 43:307-314.
20. Labaron, P., N. Parthuisot, and P. Catala. 1998. Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol. 64:1725-1730.
21. Marie, D., D. Vaulot, and F. Partensky. 1996. Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes. Appl. Environ. Microbiol. 62:1649-1655.
22. Monger, B. C., and M. Landry. 1993. Flow cytometric analysis of marine bacteria with Hoechst 33342. Appl. Environ. Microbiol. 59:905-911.
23. Packard, T. Personal communication.
24. Pomeroy, L. R., J. E. Sheldon, and W. M. Sheldon. 1994. Changes in bacterial numbers and leucine assimilation during estimations of microbial respiratory rates in seawater by the precision Winkler method. Appl. Environ. Microbiol. 60:328-332.
25. Rodriguez, G. G., D. Phipps, K. Ishiguro, and H. F. Ridgway. 1992. Use of fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1801-1808.
26. Sherr, B. F., P. del Giorgio, and E. B. Sherr. Estimating abundance and single-cell characteristics of actively respiring bacteria via the redox dye, CTC. Aquat. Microb. Ecol., in press.
27. Sherr, E. B., B. F. Sherr, and C. T. Sigmon. Comparison of metabolic activity of marine bacteria during incubation with activity of in situ bacterioplankton. Submitted for publication.
28. Sieracki, M. E., P. W. Johnson, and J. M. Sieburth. 1985. The detection, enumeration and sizing of aquatic bacteria by image-analyzed epifluorescence microscopy. Appl. Environ. Microbiol. 49:799-810.
29. Sieracki, M. E., and J. M. Sieburth. 1986. Sunlight-induced growth delay of planktonic marine bacteria in filtered seawater. Mar. Ecol. Prog. Ser. 33:19-27.
30. Sieracki, M. E., and C. L. Viles. 1992. Distributions and fluorochromestaining properties of sub-micrometer particles and bacteria in the North Atlantic. Deep Sea Res. 39:1919-1929.
31. Smith, E. M. 1998. Coherence of microbial respiration rate and cell-specific bacterial activity in a coastal planktonic community. Aquat. Microb. Ecol. 16:27-35.
32. Tabor, P. S., and R. A. Neihof. 1982. Improved method for determination of respiring individual microorganisms in natural waters. Appl. Environ. Mi-crobiol. 43:1249-1255.
33. Ullrich, S., B. Karrasch, H.-G. Hoppe, K. Jeskulke, and M. Mehrens. 1996. Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride. Appl. Environ. Microbiol. 62:4587-4593.
34. Veldhuis, M. J. W., T. L. Cucci, and M. E. Sieracki. 1997. Cellular DNA content of marine phytoplankton using two new fluorochromes: taxonomic and ecological implications. J. Phycol. 33:527-541.
35. Viles, C. L., and M. E. Sieracki. 1992. Measurement of marine picoplankton cell size by using a cooled, charge-coupled device camera with image-analyzed fluorescence microscopy. Appl. Environ. Microbiol. 58:584-592.
36. Zimmermann, R., R. Iturriaga, and J. Becker-Birck. 1978. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl. Environ. Microbiol. 36:926-935.