Method for enumeration of 5-cyano-2,3-ditoyl tetrazolium chloride (CTC)-active cells and cell-specific CTC activity of benthic bacteria in riverine, estuarine and coastal sediments

Journal of Microbiological Methods

Volumn 43, Issue 3, 2001, Pages 213-222

  Proctor, Lita M ^a   Souza, Afonso C ^a  

^a FLORIDA STATE UNIVERSITY   (United States)

Author keywords

Benthic bacteria; CTC respiration; Riverine, estuarine, coastal, sediments

Indexed keywords

5 CYANO 2,3 DITOYL TETRAZOLIUM CHLORIDE; CHLOROPHYLL; PYRROLE DERIVATIVE; SURFACTANT; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL COUNT; BACTERIAL FLORA; BENTHOS; CLAY; CONTROLLED STUDY; ESTUARY; GEOGRAPHIC DISTRIBUTION; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; RIVER; SEASHORE; SEDIMENT; ULTRASOUND;

BACTERIA; BACTERIOLOGICAL TECHNIQUES; COLONY COUNT, MICROBIAL; GEOLOGIC SEDIMENTS; MICROSCOPY, FLUORESCENCE; OXYGEN CONSUMPTION; TETRAZOLIUM SALTS; WATER MICROBIOLOGY;

EID: 0035212056     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(00)00218-9     Document Type: Article

References (24)

1. Phylogenetic identification and in situ detection of individual microbial cells without cultivation
2. A new direct microscopy based method for evaluating in situ bioremediation
3. Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
4. Comparison of the fluorescent redox dye 5-cyano 2,3-dytol tetrazolium chloride with p-iodonitrotetrazolium violet to detect metabolic activity in heat-stressed Listeria monocytogenes cells
5. Viability and isolation of marine bacteria by dilution culture: Theory, procedures, and initial results
6. Relation between presence-absence of a visible nucleoid and metabolic activity in bacterioplankton cells
7. Coupling between rates of bacterial production and the abundance of metabolically active bacteria in lakes, enumerated using CTC reduction and flow cytometry
8. Enumeration of sandy sediment bacteria: Search for the optimal protocol
9. Enumeration of sandy sediment bacteria: Are the counts quantitative or relative?
10. Active versus inactive bacteria: Size dependence in a coastal marine plankton community
11. Determination of active marine bacterioplankton: A comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining
12. Evaluation of sediment slurry microcosms for modeling microbial communities in estuarine sediments
13. Physiological assessment of bacteria using fluorochromes
14. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria
15. Constancy of bacterial abundance in surficial marine sediments
16. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water
17. Coherence of microbial respiration rate and cell-specific bacterial activity in a coastal planktonic community
18. Mechanisms of INT (2-4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride) and CTC (5-cyano-2,3-ditolyl tetrazolium chloride) reduction in Escherichia coli K-12
19. Enumeration of protozoa and bacteria in muddy sediment
20. Tidal coupling between carbohydrate concentrations and bacterial activities in diatom inhabited intertidal mud flats
21. Distinguishing between living and nonliving bacteria: Evaluation of the vital stain propidium iodine and its combined use with molecular probes in aquatic samples
22. Extracellular redox activity in activated sludge
23. Optimal staining and sample storage time for direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes
24. Total counts of marine bacteria include a large fraction of non-nucleoid-containing ghosts