The Liverbeads® as a tool for the comet assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 519, Issue 1-2, 2002, Pages 163-170

  Vian, Laurence ^a   Yusuf, Ayda Trismawaty ^a   Guyomard, Claire ^b   Cano, Jean Paul ^a  

^a UNIV MONTPELLIER   (France)

^b Biopredic International   (France)

Author keywords

Comet assay; Hepatocytes; Human; Promutagen; Rat

Indexed keywords

ALGINIC ACID; BENZO[A]PYRENE; CYCLOPHOSPHAMIDE; DIMETHYLBENZ[A]ANTHRACENE; MUTAGENIC AGENT; N (2 FLUORENYL)ACETAMIDE; PROMUTAGEN; XENOBIOTIC AGENT; 7,12 DIMETHYLBENZ[A]ANTHRACENE; CARCINOGEN; DNA; MICROSPHERE; PRODRUG;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; DNA STRAND BREAKAGE; HELA CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; LIVER CELL; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; STATISTICAL SIGNIFICANCE; AGED; ANIMAL; BIOTRANSFORMATION; COMPARATIVE STUDY; DNA DAMAGE; DRUG EFFECT; FEMALE; LIVER; METABOLISM; MIDDLE AGED; SENSITIVITY AND SPECIFICITY;

2-ACETYLAMINOFLUORENE; 9,10-DIMETHYL-1,2-BENZANTHRACENE; AGED; ANIMALS; BENZO(A)PYRENE; BIOTRANSFORMATION; CARCINOGENS; COMET ASSAY; CYCLOPHOSPHAMIDE; DNA DAMAGE; DNA, NEOPLASM; FEMALE; HELA CELLS; HEPATOCYTES; HUMANS; LIVER; MALE; MICROSPHERES; MIDDLE AGED; MUTAGENS; PRODRUGS; RATS; SENSITIVITY AND SPECIFICITY;

EID: 0037179154     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(02)00137-7     Document Type: Article

References (25)

1. Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of hepatocytes
2. Viability and drug metabolism capacity of alginate-entrapped hepatocytes after cryopreservation
3. A preliminary comparison of Liverbeads™ with a conventional rat hepatocyte culture preparation: Some aspects of xenobiotic metabolism and related toxicity
4. The comet assay: What can it really tell us?
5. Single cell gel electrophoresis assay: A new technique for human biomonitoring studies
6. Comet assay in genetic ecotoxicology: A review
7. Comet assay responses as indicators of carcinogen exposure
8. Optimization and standardization of the "comet assay" for analyzing the repair of DNA damage in cells
9. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing
10. Single cell gel electrophoresis assays with human-derived hepatoma (Hep G2) cells
11. Evaluation of the single cell gel electrophoresis assay with human hepatoma (Hep G2) cells
12. In vitro detection of indirect-acting genotoxins in the comet assay using Hep G2 cells
13. Xenobiotic metabolism in epithelial cell cultures
14. An assessment of the in vitro hepatocyte micronucleus assay
15. Structure-activity relationships in the rat hepatocyte DNA-repair test for 300 chemicals
16. UDS induction by an array of standard carcinogens in human and rodent hepatocytes: Effect of cryopreservation
17. Escherichia coli MTC, a human NADPH 450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450 isoforms 1A1, 1A2, 3A4, or 3A5: Catalytic activities and mutagenicity studies
18. Biotransformation of xenobiotics
19. Specificity of cDNA-expressed human and rodent cytochrome P450s in the oxidative metabolism of the potent carcinogen 7,12-dimethylbenz[a]anthracene
20. Development of a substrate-activity based approach to identify the major human liver P450 catalysts of cyclophosphamide and ifosfamide activation based on cDNA-expressed activities and liver microsomal P450 profiles
21. Application of the in vitro rat hepatocyte micronucleus assay in genetic toxicology testing
22. Use of primary cultures of human hepatocytes in toxicology studies
23. Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens
24. Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens