Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation

Journal of Cell Biology

Volumn 155, Issue 7, 2001, Pages 1159-1172

  Howell, B J ^a   McEwen, B F ^b   Canman, J C ^a   Hoffman, D B ^a   Farrar, E M ^a   Rieder, C L ^b   Salmon, E D ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b State of New York Department of Health   (United States)

Author keywords

CENP E; Cytoplasmic dynein; Mad2; Mitosis; Mitotic spindle checkpoint

Indexed keywords

ADENOSINE TRIPHOSPHATASE INHIBITOR; ADENOSINE TRIPHOSPHATE; CENTROMERE PROTEIN E; CYTOPLASM PROTEIN; DYNACTIN; MICROTUBULE PROTEIN; PROTEIN BUB1R; PROTEIN MAD2; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CENTROMERE; CONTROLLED STUDY; DEPOLYMERIZATION; METAPHASE; MICROTUBULE ASSEMBLY; MITOSIS RATE; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN TRANSPORT; RAT; TRANSPORT KINETICS;

ANIMALS; CELL LINE; CELL POLARITY; CHROMOSOMES; DYNEIN ATPASE; KINETOCHORES; METAPHASE; MITOTIC SPINDLE APPARATUS;

ANIMALIA;

EID: 0035945356     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200105093     Document Type: Article

References (62)

1. Chromosome movement: Dynein-out at the kinetochore
2. Rough Deal and Zw10 are required for the metaphase checkpoint in Drosophila
3. Role of ATP in the regulation and stability of cytoskeletal structures
4. Overexpression of Dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution
5. The role of pre-and postanaphase microtubules in the cytokinesis phase of the cell cycle
6. Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores
7. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores
8. Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells
9. Microtubule assembly in living cells after release from nocodazole block: The effects of metabolic inhibitors, taxol and pH
10. Microtubule polymerization dynamics
11. Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2
12. Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis
13. A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function
14. Mitotic spindle poles are organized by structural and motor proteins in addition to centrosomes
15. ATP assay: Ability to distinguish cytostatic from cytocidal anticancer drug effects
16. Differential expression of a phosphoepitope at the kinetochores of moving chromosomes
17. Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase
18. Chromosome movement in mitosis requires microtubule anchorage at spindle poles
19. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
20. Microtubule dependent changes in the assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores
21. Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells
22. A new view of the spindle checkpoint
23. Rb+ influx in response to changes in energy generation effect of the regulation of the ATP content of HeLa cells
24. Force generation by microtubule assembly/disassembly in mitosis and related movements
25. Mammalian p55CDC mediates the association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase promoting complex and is involved in the regulation of anaphase onset and late mitotic events
26. Bioluminescence of cellular ATP: A new method for evaluating cytotoxic agents in vitro
27. Dynein is a transient kinetochore component whose binding is regulated by microtubule attachment, not tension
28. Identification of a human mitotic checkpoint gene: hsMAD2
29. The kinetochore of higher eukaryotes: A molecular view
30. Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset
31. A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution
32. CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells
33. Formation of spindle poles by dynein/dynactin-dependent transport of NuMA
34. How cells get the right chromosomes
35. Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint
36. Mammalian spindle orientation and position respond to changes in cell shape in a dynein-dependent fashion
37. Retrograde transport by the microtubule associated protein MAP IC
38. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber
39. The vertebrate cell kinetochore and its roles during mitosis
40. Correlative light and electron microscopy of mitotic cells in monolayer cultures
41. The checkpoint delaying anaphase in response to chromosome monorientation is mediated by an inhibitory signal produced by unattached kinetochores
42. The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants
43. The rough deal protein is a new kinetochore component for accurate chromosome segregation in Drosophila
44. CENP-E function at kinetochores is essential of chromosome alignment
45. Waiting for anaphase Mad2 and the spindle assembly checkpoint
46. Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos
47. Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoints
48. I. Effects of dinitrophenol/deoxyglucose on the live spindle
49. Conservation of the centromere/kinetochore protein ZW10
50. ZW10 helps recruit dynactin and dynein to the kinetochore
51. Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BubR1, Bub3, Cdc20, and Mad2
52. Cdc20 by the mitotic checkpoint protein BubR1
53. Cytoplasmic dynein plays a role in mammalian mitotic spindle formation
54. Spindle microtubule dynamics: Modulation by metabolic inhibitors
55. Mitotic checkpoints: From yeast to cancer
56. Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport
57. Localization of Mad2 to kinetochores depends on microtubule attachment, not tension
58. Bipolar spindle attachments affect redistributions of ZW10, a Drosophila centromere/kinetochore component required for accurate chromosome segregation
59. Recombinant p50/Dynamitin as a tool to examine the role of dynactin in intracellular processes
60. TPX2, a novel Xenopus MAP involved in spindle pole organization
61. Kinetochore dynein: Its dynamics and role in the metaphase checkpoint
62. CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint