Cholesterol addition to ER membranes alters conformation of SCAP, the SREBP escort protein that regulates cholesterol metabolism

Molecular Cell

Volumn 10, Issue 2, 2002, Pages 237-245

  Brown, Andrew J ^a   Sun, Liping ^a   Feramisco, Jamison D ^a   Brown, Michael S ^a   Goldstein, Joseph L ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

25 HYDROXYCHOLESTEROL; CHOLESTEROL; MUTANT PROTEIN; OXYSTEROL; PROTEIN; PROTEIN SCAP; STEROL REGULATORY ELEMENT BINDING PROTEIN; TRYPSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CHOLESTEROL METABOLISM; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DEGRADATION; REGULATORY MECHANISM;

ANIMALIA;

EID: 0036671360     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00591-9     Document Type: Article

References (24)

1. A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood
2. Cholesteryl ester formation in cultured human fibroblasts: Stimulation by oxygenated sterols
3. Regulation of receptor function by cholesterol
4. Bile acid and sterol solubilization in 2-hydroxyproply-β-cyclodextrin
5. Transport-dependent proteolysis of SREBP: Relocation of Site-1 protease from Golgi to ER obviates the need for SREBP transport to Golgi
6. Cholesterol as modulator of receptor function
7. Receptor-mediated endocytosis of LDL in cultured cells
8. Mutant mammalian cells as tools to delineate the sterol regulatory element-binding protein pathway for feedback regulation of lipid synthesis
9. Hairpin orientation of sterol regulatory element binding protein-2 in cell membranes as determined by protease protection
10. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage activating protein (SCAP)
11. Regulated cleavage of sterol regulatory element binding proteins (SREBPs) requires sequences on both sides of the endoplasmic reticulum membrane
12. Cellular cholesterol efflux mediated by cyclodextrins
13. Alteration of the myometrial plasma membrane cholesterol content with cylodextrin modulates the binding affinity of the oxytocin receptor
14. Quantitation of the pool of cholesterol associated with acyl-CoA: Cholesterol acyltransferase in human fibroblasts
15. Sterols regulate processing of carbohydrate chains of wild-type SREBP cleavage-activating protein (SCAP), but not sterol-resistant mutants Y298C or D443N
16. Topology of SREBP cleavage-activating protein, a polytopic membrane protein with a sterol-sensing domain
17. Sterols regulate cycling of SREBP cleavage-activating protein (SCAP) between endoplasmic reticulum and Golgi
18. Regulated step in cholesterol feedback localized to budding of SCAP from ER membranes
19. Failure to cleave sterol regulatory element-binding proteins (SREBPs) causes cholesterol auxotrophy in Chinese hamster ovary cells with genetic absence of SREBP cleavage-activating protein
20. Identification of complexes between the COOH-terminal domains of sterol regulatory element binding proteins (SREBPs) and SREBP cleavage-activating protein (SCAP)
21. SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis
22. Sterol-resistant transcription in CHO cells caused by gene rearrangement that truncates SREBP-2
23. Overexpression of membrane domain of SCAP prevents sterols from inhibiting SCAP/SREBP exit from endoplasmic reticulum