A new role for BiP: Closing the aqueous translocon pore during protein integration into the ER membrane

Journal of Cell Biology

Volumn 156, Issue 2, 2002, Pages 261-270

  Haigh, Nora G ^a   Johnson, Arthur E ^a,b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b TEXAS A M COLLEGE OF MEDICINE   (United States)

Author keywords

BiP; Endoplasmic reticulum; Fluorescence spectroscopy; Protein integration; Translocon

Indexed keywords

ADENOSINE TRIPHOSPHATE; FLUORESCENT DYE; GLUCOSE REGULATED PROTEIN 78; MEMBRANE PROTEIN; TRANSLOCON; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MEMBRANE TRANSPORT; CELL ORGANELLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CYTOSOL; ENDOPLASMIC RETICULUM; GENE FUNCTION; MAMMAL CELL; MICROSOME; NONHUMAN; POROSITY; PRIORITY JOURNAL; PROTEIN TRANSPORT; RIBOSOME;

ADENOSINE TRIPHOSPHATE; ANIMALS; CARRIER PROTEINS; CRICETINAE; ENDOPLASMIC RETICULUM; HEAT-SHOCK PROTEINS; INTRACELLULAR MEMBRANES; IODINE; MICROSOMES; MODELS, BIOLOGICAL; MOLECULAR CHAPERONES; NAD; PERMEABILITY; PROTEIN BIOSYNTHESIS; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; RIBOSOMES; SOLUBILITY; SPECTROMETRY, FLUORESCENCE;

ANIMALIA; MAMMALIA; PHOCIDAE;

EID: 0037148535     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200110074     Document Type: Article

References (47)

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12. The lumenal domain of Sec63p stimulates the ATPase activity of BiP and mediates BiP recruitment to the translocon in Saccharomyces cerevisiae
13. Fluorescence lifetime and quenching studies on some interesting diphenylhexatriene membrane probes
14. The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation
15. Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore
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21. The aqueous pore through the translocon has a diameter of 40-60 Å during cotranslational protein translocation at the ER membrane
22. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation
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44. Molecular characterization of a novel mammalian DnaJ-like Sec63p homolog
45. Homologs of the yeast Sec complex subunits Sec62p and Sec63p are abundant proteins in dog pancreas microsomes
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47. Sec63p and Kar2p are required for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo