Sec63p and Kar2p are recquired for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo

EMBO Journal

Volumn 20, Issue 1-2, 2001, Pages 262-271

  Young, B P ^a   Craven, R A ^a   Reid, P J ^a   Willer, M ^a   Stirling, C J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Endoplasmic reticulum; Kar2p; Sec63p; Signal recognition particle; Yeast

Indexed keywords

PROTEIN; PROTEIN KAR2P; PROTEIN SEC63P; SIGNAL RECOGNITION PARTICLE; UNCLASSIFIED DRUG; CARRIER PROTEIN; FUNGAL PROTEIN; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 70; KAR2 PROTEIN, YEAST; MEMBRANE PROTEIN; MESYLIC ACID ETHYL ESTER; METHIONINE; SACCHAROMYCES CEREVISIAE PROTEIN; SEC63 PROTEIN, S CEREVISIAE;

ARTICLE; CHANNEL GATING; ENDOPLASMIC RETICULUM; GENETIC SELECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; SIGNAL TRANSDUCTION; YEAST; CELL MEMBRANE; GENETICS; GENOTYPE; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MUTAGENESIS; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE;

CELL MEMBRANE; ENDOPLASMIC RETICULUM; ETHYL METHANESULFONATE; FUNGAL PROTEINS; GENOTYPE; HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; METHIONINE; MUTAGENESIS; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL RECOGNITION PARTICLE;

EID: 0035862963     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.1.262     Document Type: Article

References (46)

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3. BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast ER
4. Transcriptional regulation of the MET3 gene of Saccharomyces cerevisiae
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10. A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation
11. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation
12. Prepro-carboxypeptidase Y and a truncated form of pre-invertase, but not full-length pre-invertase, can be posttranslationally translocated across microsomal vesicle membranes from S. cerevisiae
13. Yeast/ E. coli shuttle vectors with multiple unique restriction sites
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15. A posttargeting signal sequence recognition event in the ER membrane
16. Both lumenal and cytosolic gating of the aqueous ER translocon pore are regulated from inside the ribosome during membrane protein integration
17. Additional modules for versatile and economical PCR-based gene deletion and modification in S. cerevisiae
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20. Protein transport by purified yeast sec complex and Kar2p without membranes
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22. Mammalian Sec61 is associated with Sec62 and Sec63
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25. Signal sequences specify the targeting route to the endoplasmic reticulum membrane
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28. Genetic analysis of small nuclear RNA in S. cerevisiae: Viable sextuplet mutants
29. Sec61p serves multiple roles in secretory precursor binding and translocation into the endoplasmic reticulum membrane
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31. Structure, biosynthesis, and localization of DPAP B, an integral membrane glycoprotein of the yeast vacuole
32. Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast
33. A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, and E. coli heat shock protein
34. Sec61p and BiP directly facilitate polypeptide translocation into the ER
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36. Role of Sec61α in the regulated transfer of the ribosome-nascent chain complex from the signal recognition particle to the translocation channel
37. The S.cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19
38. Protein translocation mutants defective in the insertion of integral membrane proteins into the ER
39. Elevated recombination rates in transcriptionally active DNA
40. Homologs of the yeast sec complex subunits sec62p and sec63p are abundant proteins in dog pancreas microsomes
41. LHS1 and SIL1 provide a lumenal function that is essential for protein translocation into the endoplasmic reticulum
42. Loss of Bip/GRP78 function blocks translocation of secretory proteins in yeast
43. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
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46. Distinct domains within yeast sec61p involved in post-translational translocation and protein dislocation