Encapsulation-induced aggregation and loss in activity of γ-chymotrypsin and their prevention

Journal of Controlled Release

Volumn 81, Issue 3, 2002, Pages 307-319

  Castellanos, Ingrid J ^a   Cruz, Gloydian ^a   Crespo, Rubén ^a   Griebenow, Kai ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

Excipients; Microencapsulation; Protein aggregation; Protein stabilization; Solid in oil in water (s o w) encapsulation procedure

Indexed keywords

CHYMOTRYPSIN; MACROGOL; MICROSPHERE; POLYGLACTIN; TREHALOSE;

ARTICLE; DRUG ACTIVITY; ENCAPSULATION; ENZYME ACTIVITY; FREEZE DRYING; INFRARED SPECTROMETRY; PRIORITY JOURNAL; SENSOR;

CHYMOTRYPSIN; DRUG STABILITY; EXCIPIENTS; LACTIC ACID; MICROSPHERES; POLYETHYLENE GLYCOLS; POLYGLYCOLIC ACID; POLYMERS; PROTEIN STRUCTURE, SECONDARY; TREHALOSE;

EID: 0037124472     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-3659(02)00073-1     Document Type: Article

References (46)

1. Biomaterials in drug delivery and tissue engineering: One laboratory's experience
2. Protein instability in poly(lactic-co-glycolic acid) microparticles
3. Recent trends in the stabilization of proteins upon encapsulation in and release from bioerodible polymers
4. Protein delivery from poly(lactic-co-glycolic acid) biodegradable microspheres: Release kinetics and stability issues
5. The effect of a water/organic solvent interface on the structural stability of lysozyme
6. Microencapsulation of rh-erythropoietin, using biodegradable poly(D,L-lactide-co-glycolide): Protein stability and the effects of stabilizing excipients
7. Erythropoietin loaded microspheres prepared from biodegradable LPLG-PEO-LPLG triblock copolymers: Protein stabilization and in-vitro release properties
8. Protein behavior at the water/methylene chloride interface
9. Protein instability toward organic solvent/water emulsification: Implications for protein microencapsulation into microspheres
10. Stabilization of proteins against methylene chloride water interface-induced denaturation and aggregation
11. Microencapsulation of human growth hormone within biodegradable polyester microspheres: Protein aggregation stability and incomplete release mechanism
12. 2 interface: Enzyme unfolding and aggregation and its prevention by polyols
13. Encapsulation of bovine serum albumin in poly(lactide-co-glycolide) microspheres by the solid-in-oil-in-water technique
14. Prevention of structural perturbations and aggregation upon encapsulation of bovine serum albumin into poly(lactide-co-glycolide) microspheres using the solid-in-oil-in-water technique
15. Protein encapsulation into biodegradable microspheres by a novel S/O/W emulsion method using poly(ethylene glycol) as a protein micronization adjuvant
16. The preparation and evaluation of poly(ε-caprolactone) microparticles containing both a lipophilic and a hydrophilic drug
17. Development and in vitro characterization of vascular endothelial growth factor (VEGF)-loaded poly(DL-lactic-co-glycolic acid)/poly (ethylene glycol) microspheres using a solid encapsulation/single emulsion/solvent extraction technique
18. Fabrication of microspheres using blends of poly(ethylene adipate) and poly(ethylene adipate)/poly(hydroxybutyrate-hydroxyvalerate) with poly(caprolactone): Incorporation and release of bovin serum albumin
19. Effect of primary emulsions on microsphere size and protein-loading in the double emulsion process
20. Formation and isolation of spherical fine protein microparticles through lyophilization of protein-poly(ethylene glycol) aqueous mixture
21. Stable formulations of recombinant human growth hormone and interferon-γ for microencapsulation in biodegradable microspheres
22. Relationship between conformational stability and lyophilization-induced structural changes in chymotrypsin
23. FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres
24. A sensitive new substrate for chymotrypsin
25. Structure-guided encapsulation of bovine serum albumin in poly(DL-lactic-co-glycolic)acid
26. On the structural preservation of recombinant human growth hormone in a dried film of a synthetic biodegradable polymer
27. Application of FTIR spectroscopy to probe and improve protein structure in sustained release devices, Internet
28. Reduction of structural perturbations in bovine serum albumin by non-aqueous microencapsulation
29. Non-aqueous encapsulation of excipient-stabilized spray freeze-dried BSA into poly(lactide-co-glycolide) microspheres results in release of native protein
30. Gamma-chymotrypsin is a complex of alpha-chymotrypsin with its own autolysis products
31. Counteracting effects of thiocyanate and sucrose on chymotrypsinogen secondary structure and aggregation during freezing, drying, and rehydration
32. Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers
33. Use of infrared spectroscopy to assess secondary structure of human growth hormone within biodegradable microspheres
34. Lysozyme distribution and conformation in a biodegradable polymer matrix as determined by FTIR techniques
35. Improving stability and release kinetics of microencapsulated tetanus toxoid by co-encapsulation of additives
36. Diphtheria and tetanus toxoid microencapsulation into conventional and end-group alkylated PLA/PLGAs
37. Protein release from poly(lactic-co-glycolic acid) microspheres: Protein stability problems
38. Assessment of protein release kinetics, stability and protein polymer interaction of lysozyme encapsulated in poly(DL-lactide-co-glycolide) microspheres
39. Solid-phase aggregation of proteins under pharmaceutically relevant conditions
40. Lyophilization-induced reversible structural changes in proteins
41. Infrared spectroscopic studies of lyophilization-induced and temperature-induced protein aggregation
42. Infrared spectra and resonance interaction of amide-I vibration of parallel-chain and pleated sheet
43. Why does PEG 400 co-encapsulation improve NGF stability and release from PLGA biodegradable microspheres?
44. Encapsulation and stabilization of nerve growth factor into poly(lactic-co-glycolic) acid microspheres
45. In vitro and in vivo evaluation of a somatostatin analogue released from PLGA microspheres
46. Stabilizing insulin-like growth factor-I in poly(DL-lactide-co-glycolide) microspheres