Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats

EMBO Journal

Volumn 21, Issue 12, 2002, Pages 2843-2853

  Bayliss, Richard ^a   Leung, Sara W ^a   Baker, Rosanna P ^a   Quimby, B Booth ^a   Corbett, Anita H ^a   Stewart, Murray ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

FxFG repeats; NTF2; Nuclear trafficking

Indexed keywords

CARRIER PROTEIN; FUNGAL PROTEIN; KARYOPHERIN; MUTANT PROTEIN; NUCLEAR TRANSPORT FACTOR 2; NUCLEOPORIN; PROTEIN P15; RAN PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CRYSTAL STRUCTURE; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT;

ACTIVE TRANSPORT, CELL NUCLEUS; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CARRIER PROTEINS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; FUNGAL PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR PORE COMPLEX PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; PEPTIDES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RATS; SEQUENCE ALIGNMENT;

EID: 0037124352     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf305     Document Type: Article

References (42)

1. Proteomic analysis of nucleoporin interacting proteins
2. Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP
3. The molecular mechanism of transport of macromolecules through nuclear pore complexes
4. Structural basis for the interaction between FxFG nucleoporin repeats and importin-β in nuclear trafficking
5. Crystallization and initial X-ray diffraction characterization of complexes of FxFG nucleoporin repeats with nuclear transport factors
6. Gradient of increasing affinity of importin-β for nucleoporin along the pathway of nuclear import
7. C-H…π interactions in proteins
8. Free R value: A novel statistical quality for assessing the accuracy of crystal structures
9. Clystallography and NMR system: A new software suite for macromolecular structure determination
10. The 1.6 Å resolution crystal structure of nuclear transport factor 2 (NTF2)
11. Dissecting the interactions between NTF2, RanGDP and the nucleoporin xFxFG repeats
12. Separate binding sites on nuclear transport factor 2 (NTF2 for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsplp
13. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP Ran
14. The CCP4 suite: Programs for protein crystallography
15. The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo
16. An extensively modified version of MolScript that includes greatly enhanced colouring capabilities
17. Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/pl5 mRNA nuclear export factor
18. Transport between the cell nucleus and the cytoplasm
19. Improved methods for building protein models in electron density maps and for locating errors in these models
20. Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2
21. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
22. Nucleocytoplasmic transport: Ran, beta and beyond
23. Selective disruption of nuclear import by a functional mutant nuclear carrier protein
24. Raster3D: Photorealistic molecular graphics
25. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore protein p62
26. Functional analysis of the hydrophobic patch on nuclear transport factor 2 involved in interactions with the nuclear pore in vivo
27. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
28. Kinetic analysis of translocation through nuclear pore complexes
29. NTF2 mediates nuclear import of Ran
30. Pores for thought: Nuclear pore complex proteins
31. The yeast nuclear pore complex: Composition, architecture, and transport mechanism
32. The nuclear pore complex: A protein machine bridging the nucleus and cytoplasm
33. Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors
34. Nuclear import of Ran is mediated by the transport factor NTF2
35. Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)
36. Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
37. Molecular mechanism of translocation through nuclear pore complexes during nuclear protein import
38. The GLFG regions of Nup116p and Nup110p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex
39. Nuclear pores and nuclear assembly
40. LIGPLOT: A program to generate schematic diagrams of protein-ligand interactions