Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors

Current Biology

Volumn 8, Issue 25, 1998, Pages 1376-1386

  Shah, Sundeep ^a   Forbes, Douglass J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0032585582     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(98)00018-9     Document Type: Article

References (56)

1. 1. Dingwall C, Laskey RA: Nuclear targeting sequences - a consensus? Trends Biochem Sci 1991, 16:478-481.
2. 2. Pollard VW, Michael WM, Nakielny S, Siomi MC, Wang F, Dreyfuss G: A novel receptor-mediated nuclear protein import pathway. Cell 1996, 86:985-994.
3. 3. Michael WM, Eder PS, Dreyfuss G: The K nuclear shuttling domain: a novel signal for nuclear import and nuclear export in the hnRNP K protein. EMBO J 1997, 16:3587-3598.
4. 4. Jakel S, Gorlich D. Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. EMBO J 1998, 17:4491-4502.
5. 5. Fischer U, Huber J, Boelens WC, Mattaj IW, Lührmann R: The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 1995, 82:475-483.
6. 6. Wen W, Meinkoth JL, Tsien RY, Taylor SS: Identification of a signal for rapid export of proteins from the nucleus. Cell 1995, 82:463-473.
7. 7. Pasquinelli AE, Powers MA, Lund E, Forbes D, Dahlberg JE: Inhibition of mRNA export in vertebrate cells by nuclear export signal conjugates. Proc Natl Acad Sci USA 1997, 94:14394-14399.
8. 8. Gorlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, et al.: A novel Class of RanGTP binding proteins. J Cell Biol 1997, 138:65-80.
9. 9. Kutay U, Lipowsky G, Izaurralde E, Bischoff FR, Schwarzmaier P, Hartmann E, Gorlich D: Identification of a tRNA-specific nuclear export receptor. Mol Cell 1998, 1:359-369.
10. 10. Arts G-J, Fornerod M, Mattaj IW: Identification of a nuclear export receptor for tRNA. Curr Biol 1998, 8:305-314.
11. 11. Kutay U, Bischoff FR, Kostka S, Kraft R, Gorlich D: Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell 1997, 90:1061-1071.
12. 12 Stade K, Ford CS, Guthrie C, Weis K: Exportin 1 (Crm1p) is an essential nuclear export factor. Cell 1997, 90:1041-1050.
13. 13. Fornerod M, Ohno M, Yoshida M, Mattaj I: CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 1997, 90:1051-1060.
14. 14. Gorlich D: Nuclear protein import. Curr Opin Cell Biol 1997, 9:412-419.
15. 15. Nakielny S, Dreyfuss G: Nuclear export of proteins and RNAs. Curr Opin Cell Biol 1997, 9:420-429.
16. 16. Adam EJ, Adam SA: Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J Cell Biol 1994, 125:547-555.
17. 17. Gorlich D, Prehn S, Laskey RA, Hartmann E: Isolation of a protein that is essential for the first step of nuclear protein import. Cell 1994, 79:767-778.
18. 18. Imamoto N, Shimamoto T, Takao T, Tachibana T, Kose S, Matsubae M, et al.: In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import. EMBO J 1995, 14:3617-3626.
19. 19. Radu A, Blobel G, Moore MS: Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc Natl Acad Sci USA 1995, 92:1769-1773.
20. 20. Rexach M, Blobel G: Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 1995, 83:683-692.
21. 21. Melchior F, Paschal B, Evans J, Gerace L: Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J Cell Biol 1993, 123:1649-1659.
22. 22. Moore MS, Blobel G: The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 1993, 365:661-663.
23. 23. Moore MS, Blobel G: Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc Natl Acad Sci USA 1994, 91:10212-10216.
24. 24. Paschal BM, Gerace L: Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J Cell Biol 1995, 129:925-37.
25. 25. Izaurralde E, Kutay U, von Kobbe C, Mattaj IW, Gorlich D: The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J 1997, 16:6535-6547.
26. 26. Yaseen NR, Blobel G: Cloning and characterization of human karyopherin beta3. Proc Natl Acad Sci USA 1997, 94:4451-4456.
27. 27. Seedorf M, Silver PA: Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci USA 1997, 94:8590-8595.
28. 28. Ullman KS, Powers MA, Forbes DJ: Nuclear export receptors: from importin to exportin. Cell 1997, 90:967-970.
29. 29. Ohno M, Fornerod M, Mattaj IW: Nucleocytoplasmic transport: the last 200 nanometers. Cell 1998, 92:327-336.
30. 30. Dworetzky SI, Lanford RE, Feldherr CM: The effects of variations in the number and sequence of targeting signals on nuclear uptake. J Cell Biol 1988, 107:1279-1287.
31. 31. Doye V, Hurt E: From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 1997, 9:401-411.
32. 32. Ris H: High-resolution field-emission scanning electron microscopy of nuclear pore complex. Scanning 1997, 19:368-375.
33. 33. Cordes VC, Reidenbach S, Rackwitz HR, Franke WW: Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments. J Cell Biol 1997, 136:515-529.
34. 34. Zimowska G, Aris JP, Paddy MR: A Drosophila Tpr protein homolog is localized both in the extrachromosomal channel network and to nuclear pore complexes. J Cell Sci 1997, 110:927-944.
35. 35. Pante N, Aebi U: The nuclear pore complex. J Cell Biol 1993, 122:977-984.
36. 36. Cordes VC, Reidenbach S, Kohler A, Stuurman N, van Driel R, Franke WW: Intranuclear filaments containing a nuclear pore complex protein. J Cell Biol 1993, 123:1333-1344.
37. 37. Shah S. Tugendreich S. Forbes D: Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr. J Cell Biol 1998, 141:31-49.
38. 38. Bastos R, Lin A, Enarson M, Burke B: Targeting and function in mRNA export of nuclear pore complex protein Nup153. J Cell Biol 1996, 134:1141-1156.
39. 39. Fornerod M, van Deursen J, van Baal S, Reynolds A, Davis D, Murti KG, et al.: The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J 1997, 16:807-816.
40. 40. Delphin C, Guan T, Melchior F, Gerace L: RanGTP targets p97 to RanBP2, a filamentous protein localized at the cytoplasmic periphery of the nuclear pore complex. Mol Biol Cell 1997, 8:2379-2390.
41. 41. Saitoh H, Cooke CA, Burgess WH, Earnshaw WC, Dasso M: Direct and indirect association of the small GTPase ran with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts. Mol Biol Cell 1996, 7:1319-1334.
42. 42. Bonifaci N, Moroianu J, Radu A, Blobel G: Karyopherin beta2 mediates nuclear import of a mRNA binding protein. Proc Natl Acad Sci USA 1997, 94:5055-5060.
43. 43. Rexach M, Blobel G: Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 1995, 83:683-692.
44. 44. Iovine MK, Wente SR: A nuclear export signal in Kap95p is required for both recycling the Import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol 1997, 137:797-811.
45. 45. Percipalle P, Clarkson WD, Kent HM, Rhodes D, Stewart M: Molecular interactions between the importin alpha/beta heterodimer and proteins involved in vertebrate nuclear protein import. J Mol Biol 1997, 266:722-732.
46. 46. McMorrow I, Bastos R, Horton H, Burke B: Sequence analysis of a cDNA encoding a human nuclear pore complex protein, hnup153. Biochim Biophys Acta 1994, 1217:219-223.
47. 47. Sukegawa J, Blobel G: A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm. Cell 1993, 72:29-38.
48. 48. Adam SA, Marr RS, Gerace L: Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J Cell Biol 1990, 111:807-816.
49. 49. Finlay DR, Newmeyer DD, Price TM, Forbes DJ: Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores. J Cell Biol 1987, 104:189-200.
50. 50. Moore MS: Ran and nuclear transport J Biol Chem 1998, 273:22857-22860.
51. 51. Richards SA, Carey KL, Macara IG: Requirement of guanosine triphosphate-bound Ran for signal-mediated nuclear protein export. Science 1997, 276:1842-1844.
52. 52. Finlay DR, Meier E, Bradley P, Horecka J, Forbes DJ: A complex of nuclear pore proteins required for pore function. J Cell Biol 1991, 114:169-183.
53. 53. Meier E, Miller BR, Forbes DJ: Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation. J Cell Biol 1995, 129:1-14.
54. 54. Macaulay C, Forbes DJ: Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTPγS, and BAPTA. J Cell Biol 1996, 132:5-20.
55. 55. Ullman KS, Shah S, Powers MA, Forbes DJ: The nucleoporin Nup153 plays a critical role in multiple types of nuclear export. Mol Biol Cell, in press.
56. 56. Newmeyer DD, Forbes DJ: Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation. Cell 1988, 52:641-653.