Transition state stabilization by a catalytic RNA

Science

Volumn 298, Issue 5597, 2002, Pages 1421-1424

  Rupert, Peter B ^a   Massey, Archna P ^a   Sigurdsson, Snorri Th ^a   Ferré D'Amaré, Adrian R ^a  

^a USA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; HYDROGEN BONDS; PHOSPHATES;

TRANSESTERIFICATION;

RNA;

FUNCTIONAL GROUP; PHOSPHATE; RIBOZYME; VANADIC ACID;

RNA;

ARTICLE; CATALYSIS; COMPLEX FORMATION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; HYDROGEN BOND; PRIORITY JOURNAL; RNA CLEAVAGE; RNA STABILITY; TRANSESTERIFICATION;

BINDING SITES; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; LIGANDS; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; OXYGEN; RNA, CATALYTIC; VANADATES;

EID: 0037111875     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1076093     Document Type: Article

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38. We thank the staff of beamlines 5.0.1 and 5.0.2 of the Advanced Light Source (ALS), Lawrence Berkeley National Laboratory, for data collection support; J. Bolduc, P. Heath, and B. Shen for crystallographic and computational support; P. Gafken for mass spectrometry; and J. Simon, B. Stoddard, and G. Varani for discussions. This work was supported by the NIH (grants GM63576 and RR15943 to A.R.F. and GM56947 to S.Th.S.). P.B.R. is a postdoctoral trainee of the Chromosome Metabolism and Cancer training grant from the National Cancer Institute to the Fred Hutchinson Cancer Research Center (FHCRC). Access to ALS beamlines 5.0.1 and 5.0.2 as part of the principal research consortium was funded by general support from the FHCRC. A.R.F. is a Rita Allen Foundation Scholar. Coordinates and structure factors have been deposited with the Protein Data Bank (accession codes 1M5K, 1M50, 1M5V, and 1M5P for the methoxy, vanadate, all-RNA, and 5′-chloro structures, respectively).