The tumor vascular targeting agent combretastatin A-4-phosphate induces reorganization of the actin cytoskeleton and early membrane blebbing in human endothelial cells

Blood

Volumn 99, Issue 6, 2002, Pages 2060-2069

  Kanthou, Chryso ^a,b   Tozer, Gillian M ^a  

^a Gray Cancer Institute   (United Kingdom)

^b MOUNT VERNON HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; COMBRETASTATIN A4; CYTOSKELETON PROTEIN; F ACTIN; MITOGEN ACTIVATED PROTEIN KINASE 1; MYOSIN ADENOSINE TRIPHOSPHATASE; MYOSIN LIGHT CHAIN; RHO KINASE;

ARTICLE; CELL FUNCTION; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; DEPOLYMERIZATION; ENDOTHELIUM CELL; ENZYME ACTIVATION; ENZYME INHIBITION; FOCAL ADHESION; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; STRESS FIBER;

EID: 0037085753     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood.V99.6.2060     Document Type: Article

References (67)

1. The tumour vasculature as a target for cancer therapy
2. The tumour microcirculation as a target in cancer therapy: A clearer perspective
3. Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A4
4. Antineoplastic agents 322: Synthesis of combretastatin A-4 prodrugs
5. Combretastatin A-4, an agent that displays potent and selective toxicity toward tumor vasculature
6. Combretastatin A-4 phosphate as a tumor vascular targeting agent: Early effects in tumors and normal tissues
7. Anti-vascular approaches to solid tumour therapy: Evaluation of combretastatin A-4 phosphate
8. Endothelial permeability for macromolecules: Mechanistic aspects of pathophysiological modulation
9. Myosin light chain kinase: An actin-binding protein that regulates an ATP-dependent interaction with myosin
10. Regulation of endothelial cell gap formation and barrier dysfunction: Role of myosin light chain phosphorylation
11. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
12. The small GTP-binding protein Rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
13. Rho GTPases and their effector proteins
14. Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells
15. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase)
16. Fibroblast contractility and actin reorganization are stimulated by microtubule inhibitors
17. Microtubule depolymerization induces stress fibers, focal adhesions and DNA synthesis via the GTP-binding protein Rho
18. Regulation of the small GTP binding protein Rho by cell adhesion and the cytoskeleton
19. Focal adhesions, contractility, and signaling
20. Isolation and contraction of the stress fiber
21. Involvement of microtubules in the control of adhesion-dependent signal transduction
22. Active ERK/MAP kinase is targeted to newly forming cell-matrix adhesions by integrin engagement and v-Src
23. p38 MAP kinase activation by vascular endothelial growth factor mediates actin reorganization and cell migration in human endothelial cells
24. Regulation of actin filament dynamics by p38 map kinase-mediated phosphorylation of heat shock protein 27
25. SAPK2/p-38-dependent F-actin reorganization regulates early membrane blebbing during stress-induced apoptosis
26. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis
27. The pathways of cell death: Oncosis, apoptosis, and necrosis
28. Apoptotic membrane blebbing is regulated by myosin light chain phosphorylation
29. Peroxide-induced membrane blebbing in endothelial cells associated with glutathione oxidation but not apoptosis
30. Calcium sensitization of smooth muscle mediated by a Rhoassociated protein kinase in hypertension
31. Dephosphorylation of the catenins p120 and p100 in endothelial cells in response to inflammatory stimuli
32. Guanosine 5′-3-O-(thio)triphosphate stimulates tyrosine phosphorylation of p125FAK and paxillin in permeabilized Swiss 3T3 cells: Role of p21rho
33. Purification and assay of recombinant C3 transferase
34. Microtubules in mouse embryo fibroblasts extracted with Triton X-100
35. Intrinsic microtubule stability in interphase cells
36. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
37. Confluence of vascular endothelial cells induces cell cycle exit by inhibition of p42/p44 mitogen activated protein kinase activity
38. Regulation of the actin cytoskeleton by thrombin in human endothelial cells: Role of Rho proteins in endothelial barrier function
39. Structural and biochemical comparison of the anti-mitotic agents colchicine, combretastatin A4 and amphethinile
40. Effects of combretastatin A4 phosphate on endothelial cell morphology in vitro and relationship to tumour vascular targeting activity in vivo
41. Taxol stabilization of microtubules in vitro: Dynamics of tubulin addition and loss at opposite microtubule ends
42. Inhibition of myosin light chain phosphorylation in cultured smooth muscle cells by HA1077, a new type of vasodilator
43. Galanin, neurotensin and phorbol esters rapidly stimulate activation of mitogen activated protein kinase in small cell lung cancer cells
44. Induction of apoptosis in proliferating human endothelial cells by the tumour specific antiangiogenesis agent combretastatin A-4-P
45. Expression of a novel high molecular-weight myosin light chain kinase in endothelium
46. Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase
47. Myosin light chain kinase of smooth muscle stimulates myosin ATPase activity without phosphorylating myosin light chain
48. Butanedione monoxime suppresses contraction and ATPase activity or rabbit skeletal muscle
49. Distinct roles of ROCK (Rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts
50. Microtubules and signal transduction
51. Characterization of p190RhoGEF: A RhoA-specific guanine nucleotide exchange factor that interacts with microtubules
52. Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases
53. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells
54. Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I
55. Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing
56. Stable transfectants of smooth muscle cell line lacking the expression of myosin light chain kinase and their characterization with respect to the actomyosin system
57. Rho-Rho kinase is involved in smooth muscle cell migration through myosin light chain phosphorylation-dependent and independent pathways
58. Protein kinase C and the cytoskeleton
59. Regulation of cell motility by mitogen-activated protein kinase
60. Rho A regulates sustained smooth muscle contraction through cytoskeletal reorganization of HSP27
61. Serine phosphorylation of paxillin by heregulin-beta1: Role of p38 mitogen activated protein kinase
62. Specificity and mechanism of action of some commonly used protein kinase inhibitors
63. Cytoskeletal effects of rho-like small guanine nucleotide-binding proteins in the vascular system
64. Immunotargeting of glucose oxidase to endothelium in vivo causes oxidative vascular injury in the lungs
65. Antioxidants attenuate postischemic endothelial cell swelling and luminal membrane blebbing in cardiac capillaries
66. Effects of colchicine administration on the endothelial cells of the developing semilunar heart valves of the chick embryo
67. Mechanisms associated with tumor vascular shut-down induced by combretastatin A-4 phosphate: Intravital microscopy and measurement of vascular permeability