Distinct roles of ROCK (Rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts

Journal of Cell Biology

Volumn 150, Issue 4, 2000, Pages 797-806

  Totsukawa, Go ^a   Yamakita, Yoshihiko ^a   Yamashiro, Shigeko ^a   Hartshorne, David J ^b   Sasaki, Yasuharu ^c   Matsumura, Fumio ^a  

^a RUTGERS UNIVERSITY   (United States)

^b UNIVERSITY OF ARIZONA   (United States)

^c ASAHI CHEMICAL INDUSTRY CO   (Japan)

Author keywords

Focal adhesions; Myosin phosphatase; Myosin phosphorylation; RhoA; Stress fibers

Indexed keywords

FOCAL ADHESION KINASE; MYOSIN; MYOSIN LIGHT CHAIN; PHOSPHATASE; RHO KINASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; FIBROBLAST; MICROFILAMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM;

3T3 CELLS; ANIMALS; ANTIBODIES, MONOCLONAL; BINDING SITES; CELL ADHESION; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MYOSIN LIGHT CHAINS; MYOSIN-LIGHT-CHAIN KINASE; MYOSIN-LIGHT-CHAIN PHOSPHATASE; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RHOA GTP-BINDING PROTEIN;

ANIMALIA;

EID: 0034698841     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.4.797     Document Type: Article

References (44)

1. The control of protein phosphatase-1 by targetting subunits. The major myosin phosphatase in avian smooth muscle is a novel form of protein phosphatase-1
2. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase)
3. Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase
4. Differential subcellular localization of protein phosphatase-1 alpha, gammal, and delta isoforms during both interphase and mitosis in mammalian cells
5. Quantitative electrophoresis in polyacrylamide gels of 2-40%
6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
7. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions
8. Characterization of antibodies to smooth muscle myosin kinase and their use in localizing myosin kinase in nonmuscle cells
9. Immunological properties of myosin light-chain kinases
10. Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase
11. Rho-associated kinase of chicken gizzard smooth muscle
12. Protein phosphatase type 1 in mammalian cell mitosis: Chromosomal localization and involvement in mitotic exit
13. Association of the myosin-binding subunit of myosin phosphatase and moesin: Dual regulation of moesin phosphorylation by Rho-associated kinase and myosin phosphatase
14. Production and characterization of an antibody to myosin light chain kinase and intracellular localization of the enzyme
15. Myosin light chain phosphatase: Subunit composition, interactions and regulation
16. Interactions of the subunits of smooth muscle myosin phosphatase
17. Interactions and properties of smooth muscle myosin phosphatase
18. Phosphorylation of the large subunit of myosin phosphatase and inhibition of phosphatase activity
19. 2+ sensitization through inhibition of myosin phosphatase in rabbit trachea
20. p160ROCK, a Rho-associated coiled-coil forming protein kinase, works downstream of Rho and induces focal adhesions
21. Interaction of smooth muscle myosin phosphatase with phospholipids
22. Identification of the regions on the M110 subunit of protein phosphatase 1M that interact with the M21 subunit and with myosin
23. Clostridium difficile toxin B acts on the GTP-binding protein Rho
24. The function of myosin and myosin light chain kinase phosphorylation in smooth muscle
25. Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo
26. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
27. Regulation of the association of adducin with actin filaments by Rho-associated kinase (Rho-kinase) and myosin phosphatase
28. Regulatory light chain phosphorylation and the assembly of myosin II into the cytoskeleton of microcapillary endothelial cells
29. Phosphorylation of glial fibrillary acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase
30. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
31. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
32. Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells
33. Phosphorylation of vertebrate nonmuscle and smooth muscle myosin heavy chains and light chains
34. Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase
35. Dynamics of myosin light chain phosphorylation at Ser19 and Thr18/Ser19 in smooth muscle cells in culture
36. Regulation of cytoplasmic and smooth muscle myosin
37. Characterization of the myosin-binding subunit of smooth muscle myosin phosphatase
38. Purification and characterization of the mammalian myosin light chain phosphatase holoenzyme. The differential effects of the holoenzyme and its subunits on smooth muscle
39. Signal transduction and regulation in smooth muscle
40. Activation of myosin phosphatase targeting subunit by mitosis-specific phosphorylation
41. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension
42. Cooperation between mDial and ROCK in Rho-induced actin reorganization
43. Microinjection of non muscle and smooth muscle caldesmon into fibroblasts and muscle cells
44. In vivo phosphorylation of regulatory light chain of myosin II during mitosis of cultured cells