A scintillation proximity assay for studying inhibitors of human tau protein kinase II/cdk5 using a 96-well format

Journal of Biochemical and Biophysical Methods

Volumn 50, Issue 2-3, 2002, Pages 151-161

  Evans, David B ^a   Rank, Kenneth B ^a   Sharma, Satish K ^a  

^a PFIZER INC   (United States)

Author keywords

Alzheimer's disease; Cyclin dependent kinase 5; Scintillation proximity assay; Tau hyperphosphorylation; Tau protein kinase II; TPK II cdk5 inhibition

Indexed keywords

ADENOSINE TRIPHOSPHATE; BIOTIN; DIMETHYL SULFOXIDE; ENZYME INHIBITOR; HISTONE; PEPTIDE; PROTEIN KINASE (CALCIUM,CALMODULIN) II; RADIOISOTOPE; TAU PROTEIN;

ANALYTIC METHOD; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME INHIBITION; INCUBATION TIME; PHOSPHORYLATION; PRIORITY JOURNAL; QUANTITATIVE ASSAY;

ALZHEIMER DISEASE; AMINO ACID SEQUENCE; ANIMALS; CATTLE; CYCLIN-DEPENDENT KINASE 5; CYCLIN-DEPENDENT KINASES; HISTONES; HUMANS; OLIGOPEPTIDES; PHOSPHORUS RADIOISOTOPES; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SCINTILLATION COUNTING; SUBSTRATE SPECIFICITY;

EID: 0037016352     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(01)00184-1     Document Type: Article

References (31)

1. Alzheimer's plaques and tangles: Advances on both fronts
2. The disordered neuronal cytoskeleton in Alzheimer's disease
3. Accumulation of abnormally phosphorylated tau precedes the formation of neurofibrillary tangles in Alzheimer's disease
4. Molecular analysis of neurofibrillary degeneration in Alzheimer's disease
5. Tau protein and the neurofibrillary pathology of Alzheimer's disease
6. A preparation of Alzheimer's paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis
7. Structural stability of paired helical filaments requires microtubule-binding domains of tau: A model for self-association
8. A68: A major subunit of paired helical filaments and derivatized form of normal tau
9. Tau protein function in living cells
10. Multiple forms of human microtubule-associated protein tau: Sequences and localization in neurofibrillary tangles of Alzheimer's disease
11. Tau protein and Alzheimer's disease
12. Phosphorylation that detaches tau protein from microtubules (Ser262, Ser214) also protects it against aggregation into Alzheimer's paired helical filaments
13. The Cdk5/p35 kinase is essential for neurite growth during neuronal differentiation
14. Evidence for the participation of the neuron-specific Cdk5 activator P35 during laminin-enhanced axonal growth
15. Tau phosphorylation at serine 396 and serine 404 by human recombinant tau protein kinase II inhibits tau's ability to promote microtubule assembly
16. Hyperphosphorylated tau and neurofilament and cytoskeketal disruptions in mice overexpressing human p25, an activator of cdk5
17. Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons
18. Regulation of exocytosis by cyclin-dependent kinase 5 via phosphorylation of Munc18
19. Phosphorylation of human high molecular weight neurofilament protein (NF-H) by neuronal cyclin-dependent kinase 5 (Cdk5)
20. Phosphorylation of the high molecular weight neuronfilament protein (NF-H) by Cdk5 and p35
21. Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration
22. Proline-directed phosphorylation of human tau protein
23. p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5
24. A brain specific activator of cyclin-dependent kinase 5
25. Identification of the 23 kDa subunit of tau protein kinase II as a putative activator of cdk5 in bovine brain
26. Interaction of cyclin-dependent kinase 5 (Cdk5) and neuronal Cdk5 activator in bovine brain
27. New phosphorylation sites identified in hyperphosphorylated tau (paired helical filament-tau) from Alzheimer's disease brain using nanoelectrospray mass spectrometry
28. Characterization of the in vitro phosphorylation of human tau by tau protein kinase II (Cdk5/p20) using mass spectrometry
29. Substrate specificity characterization of a cdc2-like protein kinase purified from bovine brain
30. Calibration and assay development using the four-parameter logistic model
31. Neuronal Cdc2-like kinase: From cell cycle to neuronal function