Characterization of the in vitro phosphorylation of human tau by tau protein kinase II (cdk5/p20) using mass spectrometry

Journal of Neurochemistry

Volumn 76, Issue 4, 2001, Pages 1221-1232

  Lund, Eric T ^a   McKenna, Rosemary ^a   Evans, David B ^a   Sharma, Satish K ^a   Rodney Mathews, W ^a,b  

^a PFIZER INC   (United States)

^b NONE   (United States)

Author keywords

Alzheimer's disease; Mass spectrometry; Phosphorylation; Tau; Tau protein kinase II

Indexed keywords

AMINO ACID; PHOSPHATE; PROTEIN KINASE (CALCIUM,CALMODULIN) II; RECOMBINANT PROTEIN; TAU PROTEIN;

ALZHEIMER DISEASE; ARTICLE; DEPOLYMERIZATION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MICROTUBULE ASSEMBLY; MOLECULAR WEIGHT; NEUROFIBRILLARY TANGLE; PATHOGENESIS; PERIKARYON; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

AMINO ACID SEQUENCE; BINDING SITES; HUMANS; MOLECULAR WEIGHT; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TAU PROTEINS;

EID: 0035109902     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2001.00130.x     Document Type: Article

References (33)

1. Hyperphosphorylated tau and neurofilament and cytoskeletal disruptions in mice overexpressing human p25, an activator of cdk5
2. Alzheimer's disease hyperphosphorylated tau sequesters normal tau into tangles of filaments and disassembles microtublues
3. A procedure for in situ alkylation of cystine residues on glass fiber prior to protein microsequence analysis
4. Tau protein kinase II is involved in the regulation of normal phosphorylation state of tau protein
5. Accumulation of abnormally phosphorylated tau proceeds the formation of NFTs in Alzheimer disease
6. Contributions of mass spectrometry to peptide and protein structure
7. Molecular analysis of neurofibrillary degeneration in Alzheimer's disease
8. Tau protein function in living cells
9. Tau phosphorylation at Ser-396 and Ser-404 by human recombinant TPK II inhibits tau's ability to promote microtubule assembly
10. Tau protein and the neurofibrillary pathology of Alzheimer's disease
11. Multiple forms of human microtubule-associated protein tau: Sequences and localization in neurofibrillary tangles of Alzheimer's disease
12. A preparation of Alzheimer's paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis
13. Abnormal phosphorylation of the microtubule associated protein (tau) in Alzheimer cytososkeletal pathology
14. New phosphorylation sites identified in hyperphosphorylated tau (paired helical filament-tau) from Alzheimer's disease brain using nanoelectrospray mass spectrometry
15. The endogenous and dell cycle-dependent phosphorylation of tau protein in living cells: Implications for Alzheimer's disease
16. A serine/threonine protein kinase activity is included in the tau protein kinase fraction forming a paired helical filament epitope
17. Tau protein kinase I converts normal tau proteins into A68-like component of paired helical filaments
18. Identification of the 23 kDa subunit of tau protein kinase II as a putative activator of cdk5 in bovine brain
19. Tau protein and Alzheimer's disease
20. Alzheimer's plaques and tangles: Advances on both fronts
21. Alzheimer disease proteins (A68) share epitopes with tau but show distinct biochemical properties
22. A68: A major subunit of paired helical filaments and derivatized form of normal tau
23. Elevated neuronal Cdc2-like kinase activity in the Alzheimer's disease brain
24. The disordered neuronal cytoskeleton in Alzheimer's disease
25. Purification and characterization of a novel proline-directed protein kinase from bovine brain
26. cdc2
27. Functional studies of Alzheimer's disease tau protein
28. Evidence for phosphorylation of serine 753 in cftr using a novel metal-ion affinity resin and matrixassisted laser desorption
29. Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration
30. Phosphorylation by neuron cdc2-like protein kinase promotes dimerization of tau protein in vitro
31. Bovine prolinedirected protein kinase phosphorylates tau on sites that are abnormally phosphorylated in tau associated with Alzheimer's paired helical filaments
32. Biochemical dysfunction and memory loss: The case of Alzheimer's dementia
33. Proline-directed phosphorylation of human tau protein