Gelatin zymography and substrate cleavage assays of matrix metalloproteinase-2 in breast carcinoma cells overexpressing membrane type-1 matrix metalloproteinase

Laboratory Investigation

Volumn 82, Issue 11, 2002, Pages 1583-1590

  Ratnikov, Boris I ^a   Deryugina, Elena I ^a   Strongin, Alex Y ^a  

^a BURNHAM INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME PRECURSOR; GELATIN; GELATINASE A; INTERSTITIAL COLLAGENASE; RECEPTOR; TISSUE INHIBITOR OF METALLOPROTEINASE 2;

ARTICLE; ASSAY; AUTOLYSIS; BREAST CARCINOMA; CONTROLLED STUDY; ENZYME ACTIVATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; ZYMOGRAPHY;

EID: 0036850364     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.LAB.0000038555.67772.DB     Document Type: Article

References (37)

1. Bassi DE, Lopez De Cicco R, Mahloogi H, Zucker S, Thomas G, and Klein-Szanto AJ (2001). Furin inhibition results in absent or decreased invasiveness and tumorigenicity of human cancer cells. Proc Natl Acad Sci USA 98:10326-10331.
2. Bigg HF, Morrison CJ, Butler GS, Bogoyevitch MA, Wang Z, Soloway PD, and Overall CM (2001). Tissue inhibitor of metalloproteinases-4 inhibits but does not support the activation of gelatinase A via efficient inhibition of membrane type 1-matrix metalloproteinase. Cancer Res 61:3610-3618.
3. Brew K, Dinakarpandian D, and Nagase H (2000). Tissue inhibitors of metalloproteinases: Evolution, structure and function. Biochim Biophys Acta 1477:267-283.
4. Brooks PC, Silletti S, von Schalscha TL, Friedlander M, and Cheresh DA (1998). Disruption of angiogenesis by PEX, a noncatalytic metalloproteinase fragment with integrin binding activity. Cell 92:391-400.
5. Deryugina EI, Bourdon MA, Luo GX, Reisfeld RA, and Strongin A (1997b). Matrix metalloproteinase-2 activation modulates glioma cell migration. J Cell Sci 110:2473-2482.
6. Deryugina EI, Bourdon MA, Reisfeld RA, and Strongin A (1998). Remodeling of collagen matrix by human tumor cells requires activation and cell surface association of matrix metalloproteinase-2. Cancer Res 58:3743-3750.
7. Deryugina EI, Luo GX, Reisfeld RA, Bourdon MA, and Strongin A (1997a). Tumor cell invasion through matrigel is regulated by activated matrix metalloproteinase-2. Anticancer Res 17:3201-3210.
8. Deryugina EI, Ratnikov B, Monosov E, Postnova TI, DiScipio R, Smith JW, and Strongin AY (2001). MT1-MMP initiates activation of pro-MMP-2 and integrin alphavbeta3 promotes maturation of MMP-2 in breast carcinoma cells. Exp Cell Res 263:209-223.
9. Goldberg GI, Marmer BL, Grant GA, Eisen AZ, Wilhelm S, and He CS (1989). Human 72-kilodalton type IV collagenase forms a complex with a tissue inhibitor of metalloproteases designated TIMP-2. Proc Natl Acad Sci USA 86:8207-8211.
10. Higashi S and Miyazaki K (1999). Reactive site-modified tissue inhibitor of metalloproteinases-2 inhibits the cell-mediated activation of progelatinase A. J Biol Chem 274: 10497-10504.
11. Hotary K, Allen E, Punturieri A, Yana I, and Weiss SJ (2000). Regulation of cell invasion and morphogenesis in a three-dimensional type I collagen matrix by membrane-type matrix metalloproteinases 1, 2, and 3. J Cell Biol 149:1309-1323.
12. Itoh Y, Ito A, Iwata K, Tanzawa K, Mori Y, and Nagase H (1998b). Plasma membrane-bound tissue inhibitor of metalloproteinases (TIMP)-2 specifically inhibits matrix metalloproteinase 2 (gelatinase A) activated on the cell surface. J Biol Chem 273:24360-24367.
13. Itoh T, Tanioka M, Yoshida H, Yoshioka T, Nishimoto H, and Itohara S (1998a). Reduced angiogenesis and tumor progression in gelatinase A-deficient mice. Cancer Res 58:1048-1051.
14. Leber TM and Balkwill FR (1997). Zymography: A single-step staining method for quantitation of proteolytic activity on substrate gels. Anal Biochem 249:24-28.
15. Lim YT, Sugiura Y, Laug WE, Sun B, Garcia A, and DeClerck YA (1996). Independent regulation of matrix metalloproteinases and plasminogen activators in human fibrosarcoma cells. J Cell Physiol 167:333-340.
16. Mackay AR, Ballin M, Pelina MD, Farina AR, Nason AM, Hartzler JL, and Thorgeirsson UP (1992). Effect of phorbol ester and cytokines on matrix metalloproteinase and tissue inhibitor of metalloproteinase expression in tumor and normal cell lines. Invasion Metastasis 12:168-184.
17. McQuibban GA, Gong JH, Tam EM, McCulloch CA, Clark-Lewis I, and Overall CM (2000). Inflammation dampened by gelatinase A cleavage of monocyte chemoattractant protein-3. Science 289:1202-1206.
18. Miyazaki K, Funahashi K, Numata Y, Koshikawa N, Akaogi K, Kikkawa Y, Yasumitsu H, and Umeda M (1993). Purification and characterization of a two-chain form of tissue inhibitor of metalloproteinases (TIMP) type 2 and a low molecular weight TIMP-like protein. J Biol Chem 268:14387-14393.
19. Morgunova E, Tuuttila A, Bergmann U, and Tryggvason K (2002). Structural insight into the complex formation of latent matrix metalloproteinase 2 with tissue inhibitor of metalloproteinase 2. Proc Natl Acad Sci USA 99:7414-7419.
20. Morrison CJ, Butler GS, Bigg HF, Roberts CR, Soloway PD, and Overall CM (2001). Cellular activation of MMP-2 (gelatinase A) by MT2-MMP occurs via a TIMP-2-independent pathway. J Biol Chem 276:47402-47410.
21. Murphy G, Stanton H, Cowell S, Butler G, Knauper V, Atkinson S, and Gavrilovic J (1999). Mechanisms for pro matrix metalloproteinase activation. APMIS 107:38-44.
22. Nagase H and Woessner JF (1999). Matrix metalloproteinases. J Biol Chem 274:21491-21494.
23. Oliver GW, Leferson JD, Stetler-Stevenson WG, and Kleiner DE (1997). Quantitative reverse zymography: Analysis of picogram amounts of metalloproteinase inhibitors using gelatinase A and B reverse zymograms. Anal Biochem 244:161-166.
24. Overall CM, King AE, Sam DK, Ong AD, Lau TT, Wallon UM, DeClerck YA, and Atherstone J (1999). Identification of the tissue inhibitor of metalloproteinases-2 (TIMP-2) binding site on the hemopexin carboxyl domain of human gelatinase A by site-directed mutagenesis: The hierarchical role in binding TIMP-2 of the unique cationic clusters of hemopexin modules III and IV. J Biol Chem 274:4421-4429.
25. Pei D and Weiss SJ (1995). Furin-dependent intracellular activation of the human stromelysin-3 zymogen. Nature 375:244-247.
26. Ratnikov B, Deryugina E, Leng J, Marchenko G, Dembrow D, and Strongin A (2000). Determination of matrix metalloproteinase activity using biotinylated gelatin. Anal Biochem 286:149-155.
27. Rozanov DV, Deryugina EI, Ratnikov BI, Monosov EZ, Marchenko GN, Quigley JP, and Strongin AY (2001). Mutation analysis of membrane type-1 matrix metalloproteinase (MT1-MMP): The role of the cytoplasmic tail Cys-574, the active site Glu-240 and furin cleavage motifs in oligomerization, processing and self-proteolysis of MT1-MMP expressed in breast carcinoma cells. J Biol Chem 276:25705-25714.
28. Sato H, Takino T, Okada Y, Cao J, Shinagawa A, Yamamoto E, and Seiki M (1994). A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature 370:61-65.
29. Seiki M (1999). Membrane-type matrix metalloproteinases. APMIS 107:137-143.
30. Shankavaram UT, Lai WC, Netzel-Arnett S, Mangan PR, Ardans JA, Caterina N, Stetler-Stevenson WG, Birkedal-Hansen H, and Wahl LM (2001). Monocyte membrane type 1-matrix metalloproteinase: Prostaglandin-dependent regulation and role in metalloproteinase-2 activation. J Biol Chem 276:19027-19032.
31. Shofuda K, Moriyama K, Nishihashi A, Higashi S, Mizushima H, Yasumitsu H, Miki K, Sato H, Seiki M, and Miyazaki K (1998). Role of tissue inhibitor of metalloproteinases-2 (TIMP-2) in regulation of pro-gelatinase A activation catalyzed by membrane-type matrix metalloproteinase-1 (MT1-MMP) in human cancer cells. J Biochem (Tokyo) 124:462-470.
32. Sternlicht MD and Werb Z (2001). How matrix metalloproteinases regulate cell behavior. Annu Rev Cell Dev Biol 17:463-516.
33. Strongin AY, Collier I, Bannikov G, Marmer BL, Grant GA, and Goldberg GI (1995). Mechanism of cell surface activation of 72-kDa type IV collagenase: Isolation of the activated form of the membrane metalloprotease. J Biol Chem 270:5331-5338.
34. Strongin AY, Marmer BL, Grant GA, and Goldberg GI (1993). Plasma membrane-dependent activation of the 72-kDa type IV collagenase is prevented by complex formation with TIMP-2. J Biol Chem 268:14033-14039.
35. Van Wart HE and Birkedal-Hansen H (1990). The cysteine switch: A principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family. Proc Natl Acad Sci USA 87:5578-5582.
36. Will H, Atkinson SJ, Butler GS, Smith B, and Murphy G (1996). The soluble catalytic domain of membrane type 1 matrix metalloproteinase cleaves the propeptide of progelatinase A and initiates autoproteolytic activation: Regulation by TIMP-2 and TIMP-3. J Biol Chem 271:17119-17123.
37. Zucker S, Drews M, Conner C, Foda HD, DeClerck YA, Langley KE, Bahou WF, Docherty AJ, and Cao J (1998). Tissue inhibitor of metalloproteinase-2 (TIMP-2) binds to the catalytic domain of the cell surface receptor, membrane type 1-matrix metalloproteinase 1 (MT1-MMP). J Biol Chem 273: 1216-1222.