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Miller M.J., Wei S.H., Parker I., Cahalan M.D. Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science. 296:2002;1869-1873. The locomotion of fluorescently labeled T and B lymphocytes was tracked in intact lymph nodes down to 350 μm depth. Lymphocytes were highly motile at physiological temperatures. Antigen stimulation switched the behavior of T cells from random walking to the formation of stable clusters and dynamic swarms.
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Science
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