Gene expression profiling using laser capture microdissection

Expert Review of Molecular Diagnostics

Volumn 2, Issue 5, 2002, Pages 497-507

  Todd, Randy ^a   Lingen, Mark W ^a   Kuo, Winston P ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Diagnosis; Disease classification; Gene expression profiling; Laser capture microdessection

Indexed keywords

DNA; RNA;

ARTICLE; DNA FINGERPRINTING; DNA HYBRIDIZATION; DNA LIBRARY; GENE EXPRESSION; HUMAN; LASER CAPTURE MICRODISSECTION; NONHUMAN; POLYMERASE CHAIN REACTION; RNA ANALYSIS;

EID: 0036712281     PISSN: 14737140     EISSN: None     Source Type: Journal    
DOI: 10.1586/14737159.2.5.497     Document Type: Article

References (66)

1. Emerging molecular markers of cancer
2. Gene expression profiling predicts clinical outcome of breast cancer
3. Prediction of central nervous system embryonal tumour outcome based on gene expression
4. Multiclass cancer diagnosis using tumor gene expression signatures
5. Towards a novel classification of human malignancies based on gene expression patterns
6. Single-cell molecular biology: Implications for the diagnosis and treatment of neurological disease
7. In vivo gene expression profile analysis of human breast cancer progression
8. Proteornic analysis of laser capture microdissected human prostate cancer and in vitro prostate cell lines
9. Tumor classification
10. Molecular profiling of tissue samples using laser capture microdissection
11. The cutting edge in laser microdissection
12. Laser capture microdissection-generated target sample for high-density oligonucleotide array hybridization
13. Total RNA-based target design for microarray analysis of defined tumor areas
14. Applications of laser capture microdissection in genomics and proteomics
15. RT-PCR from archival single cells is a suitable method to analyse specific gene expression
16. Preservation of RNA for functional genomic studies: A multidisciplinary tumor bank protocol
17. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue
18. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology application for such samples
19. Effect of tissue processing on the ability to recover nucleic acid from specific renal tissue compartments by laser capture microdissection
20. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue
21. Methacarn fixation: A novel tool for analysis of gene expressions in paraffin-embedded tissue specimens
22. Enrichment of epithelial cells for molecular studies
23. Analysis of anterior pituitary hormone mRNA expression in immunophenotypically characterized single cells after laser capture microdissection
24. Molecular analysis of complex tissues is facilitated by laser capture microdissection
25. Laser capture microdissection-guided fluroescence in situ hybridization and flow cytometric cell cycle analysis of purified nuclei from paraffin sections
26. IFLCM laser capture microdissection of immunofluorescently defined cells for mRNA analysis
27. Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection
28. Microdissection is essential for gene expression profiling of clinically resected cancer tissues
29. Gene expression profiles of laser-captured adjacent neuronal subtypes
30. High-fidelity mRNA amplification for gene profiling
31. RNA ampification results in reproducible microarray data with slight ratio bias
32. Challenges of single-cell diagnostics: Analysis of gene expression
33. Real-time quantitative RT-PCR after laser-assisted cell picking
34. Cell type-specific mRNA quantitation in non-neoplastic tissues after laser-assisted cell picking
35. Real-time RT-PCR for the detection of βadrenoceptor messenger RNAs in small human endomyocardial biopsies
36. Laser capture microdissection analysis of gene expression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice
37. Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis
38. Gene expression profiles in human BPH: Utilization of laser-capture microdissection and quantitative real-time PCR
39. PSGR, a novel prostate-specific gene with homology to a G-protein coupled receptor, is overexpressed in prostate cancer
40. Inflammatory mediators in uveitis: Differential induction of cytokines and chemokines in Th1-versus Th2-mediated ocular inflammation
41. Laser capture microdissection and real-time reverse transcriptase/polymerase chain reaction of bronchiolar epithelium after bleomycim
42. DNA hybridization arrays for gene expression analysis of human oral cancer
43. Distinct pattern of expression of differentiation and growth-related genes in squamous cell carcinomas of the head and neck revealed by the use of laser capture microdissection and cDNA arrays
44. Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays afer laser-capture microdissection of tumor tissues and normal epithelia
45. Laser capture microdissection and microarray expression analysis of lung adenocarcinoma reveals tobacco smoking- and prognosis-related molecular profiles
46. An improved method for construction of directionally cloned cDNA libraries from microdissected cells
47. Gene expression profiles in squamous cell carcinomas of the oral cavity: Use of laser captue microdissection for the construction and analysis of stage-specific cDNA libraries
48. A new gene (rmSTG) specific for taste buds is found by laser capture microdissection
49. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
50. Principles of differential display
51. Systematic analysis of intrinsic factors affecting differential display
52. Molecular pathology and future developments
53. Death-associated protein 3 is overexpressed in invasive glioblastoma cells in vivo and in glioma cell lines with induced motility phenotype in vitro
54. P190-B, a Rho-GTPase-activating protein, is differentially expressed in terminal end buds and breast cancer
55. cDNA sequencing and analysis of POV1 (PB39): A novel gene upregulated in prostate cancer
56. Separation of samples into their constituents using gene expression data
57. Broad patterns of gene expression revealed by dustering anlaysis of tumor and normal colon tissues probed by oligonudeotide arrays
58. cDNA array hybridization after laser-assisted microdissection from nonneoplastic tissue
59. Laser capture microdissection and proteomics: Possibilities and limitation
60. MicroSAGE: A modified procedure for serial analysis of gene expression in limited amounts of tissue
61. Comprehensive transcript analysis in small quantities of mRNA by SAGE-lite
62. MiniSAGE: Gene expression profiling using serial analysis of gene expression from 1 μg total RNA
63. Proteomic profiling of the cancer microenvironment by antibody arrays
64. Cancer proteomics: From biomarker discovery to signal pathway profiling
65. Proteomic approaches within the NCI early detection research network for the discovery and identification of cancer biomarkers
66. Clinical proteomics: Personalized molecular medicine